Abstract: The new fluroscent assay has been developed for determining carbofuran residue in vegetable.The sample is extracted with acetic ether,cleaned up with activated carbon and carbofuran solid phase extracting column,hydrolyzed for 25 min in boiled water of pH8.0,derivatized with o-phthalaldehyde in carbonate buffer of pH11.25 and the mass ratio of o-phthaladehyde and carbofuran is more than 6.5∶1.Under these conditions,the linear dynamic range of carbofuran is 0.09～1.2 mg/L,detection limit is 25 μg/kg and the recoveries of spiked sample are between 82.18%～94.3%.The assay can be applied for determination of carbofuran in vegetables.

Key words: carbofuran residue, o-phthaladehyde, hydrolyzation, derivatization, fluorospectrophotometry