Abstract: In order to reveal the effects of soil indicators in different producing areas on the effective ingredients and activities of Bletilla striata, this study took B. striata and its cultivated soil from 8 producing areas in Guizhou as the research objects. 10 soil indicators, 5 functional components, 4 trace elements, tyrosinase inhibitory activity and anticoagulation activity of B. striata were determined. Redundancy analysis was used to investigate the correlation between soil indicators, functional components and activities, so as to reflect the effects of different soil indicators on the quality and activity of B. striata. The results showed that the contents of total ash, polysaccharide, total phenol, militarine and BTS from 8 different producing areas were 27.1~41.5 mg/g, 36.2%~56.4%, 1.8~4.1 mg/g, 35.4~82.6 mg/g and 8.2~12.7 mg/g, respectively. The contents of Cu, Zn, Fe and Mn were 6.21~16.51 mg/kg, 6.38~13.77 mg/kg, 124.49~253.4 mg/kg, 6.51~42.14 mg/kg, respectively. The inhibitory activity of tyrosinase was 40.2%~66.5%, and the clotting time was 236.1~306.4 s. Soil indicators had a great influence on the effective components, trace elements and activity of B. striata, among which the six soil indicators with significant influence were available zinc, pH, total nitrogen, available copper, available phosphorus and organic matter in order of contribution degree. The results of this study revealed the key soil factors affecting the quality difference of B. striata, and laid the research foundation for the standardization and scale cultivation of B. striata.

Key words: Bletilla striata, soil factor, functional composition, bioactivity, redundance analysis