微塑料和镉复合胁迫对禾花鲤标准代谢的影响

doi:10.16088/j.issn.1001-6600.2024040801

Abstract

Abstract: In recent years, microplastics (MPs) and cadmium (Cd) have attracted significant attention due to their combined toxic effects on aquatic organisms. Rice flower carp (Cyprinus carpio var. Quanzhounensis) is a characteristic local species in Quanzhou County, Guangxi, China. The effects of combined exposure to MPs and Cd on C. carpio remain unclear. The present study was performed to determine the individual (0.25, 0.5, 1.0, and 2.0 mg/L Cd or 0.25, 0.5, 1.0, and 2.0 mg/L MPs) and combined effects of MPs and Cd on the oxygen consumption rate (η_OC), ammonia excretion rate (η_AE), and O/N ratio (η_O/N) of C. carpio var. Quanzhounensis. The results showed that, under single Cd stress, the η_OC initially increased and then decreased with increasing Cd concentration, while η_AE increased, and the η_O/N continuously decreased with increasing Cd concentration. More importantly, the η_O/N was dropped below 7 when the Cd concentration exceeded 1.0 mg/L. Under single MPs stress, the η_OC and η_O/N showed a decreasing trend, which leveled off when the MPs concentration exceeded 0.5 mg/L; Similarly, the η_O/N was also dropped below 7 when the MPs concentration exceeded 1.0 mg/L; In addition, η_AE initially increased and then also exhibited a leveling-off trend when the MPs concentration exceeded 0.5 mg/L. Interestingly, compared with the single exposure groups, the change patterns of oxygen consumption rate, ammonia excretion rate and η_O/N in the co-exposure groups were similar, but with a significantly greater magnitude, indicating a synergistic effect of MPs and Cd on the standard metabolism of C. carpio. Two-way ANOVA showed that the interaction between Cd and MPs significantly affected η_OC, η_AE, and η_O/N (P>0.05). Moreover, binary logistic regression analysis revealed that Cd had a greater impact on C. carpio than that of MPs. Taken together, both single and combined MPs and Cd affect the standard metabolism of C. carpio; Synergistic toxic effects of MPs and Cd were observed in this study, and the toxicity of Cd was stronger than that of MPs on C. carpio; With increasing exposure concentration, the η_O/N less than 7, indicating that C. carpio may rely exclusively on proteins as an energy source, leading to diminished protein synthesis, which in turn hampers the increase in body weight and harmful to the health of C. carpio.

Key words: Cyprinus carpio var. Quanzhounensis, microplastics, cadmium, oxygen consumption rate, ammonia excretion rate

CLC Number: X503.225;S917.4

CHENG Chunxing, PAN Qing, WU Yangyang, WEI Jinyou, WEI Yuwei, TIAN Wenfei, JIANG Jiaoyun. Combined Effects of Microplastics and Cadmium on Standard Metabolism of Cyprinus carpio var. Quanzhounensis[J].Journal of Guangxi Normal University(Natural Science Edition), 2025, 43(1): 65-74.

References

[1] LEBRETON L C M, VAN DER ZWET J, DAMSTEEG J W, et al. River plastic emissions to the world's oceans[J]. Nature Communications, 2017, 8: 15611. DOI: 10.1038/ncomms15611.
[2] PLASTICS EUROPE. Plastics-The Facts 2020. An analysis of European latest plastic production, demand and waste data[R]. Association of Plastics Manufacturers, Brussels, Belgium, 2020.
[3] ALIMI O S, FARNER BUDARZ J, HERNANDEZ L M, et al. Microplastics and nanoplastics in aquatic environments: aggregation, deposition, and enhanced contaminant transport[J]. Environmental Science & Technology, 2018, 52(4): 1704-1724. DOI: 10.1021/acs.est.7b05559.
[4] THOMPSON R C, MOORE C J, VOM SAAL F S, et al. Plastics, the environment and human health: current consensus and future trends[J]. Philosophical Transactions of the Royal Society B: Biological Sciences, 2009, 364(1526): 2153-2166. DOI: 10.1098/rstb.2009.0053.
[5] SIPPS K, ARBUCKLE-KEIL G, CHANT R, et al. Pervasive occurrence of microplastics in Hudson-Raritan estuary zooplankton[J]. Science of the Total Environment, 2022, 817: 152812. DOI: 10.1016/j.scitotenv.2021.152812.
[6] ZHANG K N, LIANG J H, LIU T, et al. Abundance and characteristics of microplastics in shellfish from Jiaozhou Bay, China, China[J]. Journal of Oceanology and Limnology, 2022, 40(1): 163-172. DOI: 10.1007/s00343-021-0465-7.
[7] SABOROWSKI R, KOREZ Š, RIESBECK S, et al. Shrimp and microplastics: a case study with the Atlantic ditch shrimp Palaemon varians[J]. Ecotoxicology and Environmental Safety, 2022, 234: 113394. DOI: 10.1016/j.ecoenv.2022.113394.
[8] ZHANG L S, XIE Y S, ZHONG S, et al. Microplastics in freshwater and wild fishes from Lijiang River in Guangxi, southwest China[J]. Science of the Total Environment, 2021, 755(Pt 1): 142428. DOI: 10.1016/j.scitotenv.2020.142428.
[9] KINIGOPOULOU V, PASHALIDIS I, KALDERIS D, et al. Microplastics as carriers of inorganic and organic contaminants in the environment: a review of recent progress[J]. Journal of Molecular Liquids, 2022, 350: 118580. DOI: 10.1016/j.molliq.2022.118580.
[10] HU F W, SUN M, LI L, et al. Effects of environmental cadmium on cadmium accumulation, oxidative response, and microelements regulation in the liver and kidney of Hexagrammos otakii[J]. Journal of Ocean University of China, 2022, 21(2): 479-485. DOI: 10.1007/s11802-022-4969-3.
[11] CHENG C X, WU Y Y, YE Q Q, et al. Individual and combined effects of microplastics and cadmium on intestinal histology and microflora of Procypris merus[J]. Aquaculture Reports, 2023, 31: 101659. DOI: 10.1016/j.aqrep.2023.101659.
[12] JIANG Z C, GUO Z H, PENG C, et al. Heavy metals in soils around non-ferrous smelteries in China: status, health risks and control measures[J]. Environmental Pollution, 2021, 282: 117038. DOI: 10.1016/j.envpol.2021.117038.
[13] WEN Y B, LI W, YANG Z F, et al. Evaluation of various approaches to predict cadmium bioavailability to rice grown in soils with high geochemical background in the karst region, southwestern China[J]. Environmental Pollution, 2020, 258: 113645. DOI: 10.1016/j.envpol.2019.113645.
[14] 李龑, 姜晓娜, 葛彦龙, 等. 温度对两种鲤耗氧率、排氨率及窒息点的影响[J]. 淡水渔业, 2023, 53(2): 61-68. DOI: 10.13721/j.cnki.dsyy.2023.02.004.
[15] FERRARI L, EISSA B L, SALIBIÁN A. Energy balance of juvenile Cyprinus carpio after a short-term exposure to sublethal water-borne cadmium[J]. Fish Physiology and Biochemistry, 2011, 37(4): 853-862. DOI: 10.1007/s10695-011-9483-2.
[16] WU Y Y, TIAN W F, CHENG C X, et al. Effects of cadmium exposure on metabolism, antioxidant defense, immune function, and the hepatopancreas transcriptome of Cipangopaludina cathayensis[J]. Ecotoxicology and Environmental Safety, 2023, 264: 115416. DOI: 10.1016/j.ecoenv.2023.115416.
[17] WEBB S, GAW S, MARSDEN I D, et al. Biomarker responses in New Zealand green-lipped mussels Perna canaliculus exposed to microplastics and triclosan[J]. Ecotoxicology and Environmental Safety, 2020, 20: 110871. DOI: 10.1016/j.ecoenv.2020.110871.
[18] ZHENG S W, WANG W X. Disturbing ion regulation and excretion in medaka (Oryzias melastigma) gills by microplastics: Insights from the gut-gill axis[J]. Science of the Total Environment, 2023, 857(Pt 2): 159353. DOI: 10.1016/j.scitotenv.2022.159353.
[19] 潘贤辉, 周康奇, 陈忠, 等. 基于线粒体D-loop区和COI基因序列研究2个禾花鲤群体和野生鲤群体的遗传多样性与系统进化关系[J]. 淡水渔业, 2019, 49(6): 33-40. DOI: 10.13721/j.cnki.dsyy.2019.06.006.
[20] 蒋经运, 钟永旺, 冯勇翔. 乡村振兴背景下促进全州禾花鱼养殖产业发展对策研究[J]. 中国水产, 2023(8): 75-78.
[21] 周康奇, 潘贤辉, 林勇, 等. 广西禾花鲤形态性状与体质量的关系分析[J]. 河南农业科学, 2020, 49(9): 159-165. DOI: 10.15933/j.cnki.1004-3268.2020.09.020.
[22] HUANG W, SONG B, LIANG J, et al. Microplastics and associated contaminants in the aquatic environment: a review on their ecotoxicological effects, trophic transfer, and potential impacts to human health[J]. Journal of Hazardous Materials, 2021, 405: 124187. DOI: 10.1016/j.jhazmat.2020.124187.
[23] LI Y, LI J Q, XUE S Y, et al. Effects of Cu, Zn and Cd on larval development and respiratory metabolism of pacific abalone (Haliotis discus hannai)[J]. Aquaculture Research, 2020, 51(10): 4255-4266. DOI: 10.1111/are.14768.
[24] LI J, XIE X J. Inconsistent responses of liver mitochondria metabolism and standard metabolism in Silurus Meridionalis when exposed to waterborne cadmium[J]. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2018, 214: 17-22. DOI: 10.1016/j.cbpc.2018.08.003.
[25] CAO J W, XU R, WANG F H, et al. Polyethylene microplastics trigger cell apoptosis and inflammation via inducing oxidative stress and activation of the NLRP3 inflammasome in carp gills[J]. Fish & Shellfish Immunology, 2023, 132: 108470. DOI: 10.1016/j.fsi.2022.108470.
[26] HAN Y, SHI W, TANG Y, et al. Microplastics and bisphenol a hamper gonadal development of whiteleg shrimp (litopenaeus Vannamei) by interfering with metabolism and disrupting hormone regulation[J]. Science of the Total Environment, 2022, 810: 152354. DOI: 10.1016/j.scitotenv.2021.152354.
[27] SUI Y M, ZHANG T, YAO X Y, et al. Synthesized effects of medium-term exposure to seawater acidification and microplastics on the physiology and energy budget of the thick shell mussel Mytilus coruscus[J]. Environmental Pollution, 2022, 308: 119598. DOI: 10.1016/j.envpol.2022.119598.
[28] CHEN X, PENG L B, WANG D, et al. Combined effects of polystyrene microplastics and cadmium on oxidative stress, apoptosis, and Gh/igf axis in zebrafish early life stages[J]. Science of the Total Environment, 2022, 813: 152514. DOI: 10.1016/j.scitotenv.2021.152514.
[29] BANAEE M, SOLTANIAN S, SUREDA A, et al. Evaluation of single and combined effects of cadmium and micro-plastic particles on biochemical and immunological parameters of common carp (Cyprinus carpio)[J]. Chemosphere, 2019, 236: 124335. DOI: 10.1016/j.chemosphere.2019.07.066.
[30] CLIFFORD III H C, BRICK R W. Nutritional physiology of the freshwater shrimp Macrobrachium rosenbergii (de Man)-I. Substrate metabolism in fasting juvenile shrimp[J]. Comparative Biochemistry and Physiology Part A: Physiology, 1983, 74(3): 561-568. DOI: 10.1016/0300-9629(83)90548-0.
[31] PRETTO A, LORO V L, MORSCH V M, et al. Alterations in carbohydrate and protein metabolism in silver catfish (Rhamdia quelen) exposed to cadmium[J]. Ecotoxicology and Environmental Safety, 2014, 100: 188-192. DOI: 10.1016/j.ecoenv.2013.11.004.
[32] DE SILVA N A L, MARSDEN I D, GAW S, et al. Physiological and biochemical responses of the estuarine pulmonate mud snail, Amphibola crenata, sub-chronically exposed to waterborne cadmium[J]. Aquatic Toxicology, 2023, 256: 106418. DOI: 10.1016/j.aquatox.2023.106418.
[33] ZHANG C S, LI F H, XIANG J H. Acute effects of cadmium and copper on survival, oxygen consumption, ammonia-N excretion, and metal accumulation in juvenile Exopalaemon carinicauda[J]. Ecotoxicology and Environmental Safety, 2014, 104: 209-214. DOI: 10.1016/j.ecoenv.2014.01.008.
[34] ZHANG C S, JIN Y, YU Y, et al. Cadmium-induced oxidative stress, metabolic dysfunction and metal bioaccumulation in adult palaemonid shrimp Palaemon macrodactylus (Rathbun, 1902)[J]. Ecotoxicology and Environmental Safety, 2021, 208: 111591. DOI: 10.1016/j.ecoenv.2020.111591.
[35] WIDDOWS J. Physiological indexes of stress in Mytilus edulis[J]. Journal of the Marine Biological Association of the United Kingdom, 1978, 58: 125-142. DOI: 10.1017/S0025315400024450.
[36] CHANDURVELAN R, MARSDEN I D, GAW S, et al. Impairment of green-lipped mussel (Perna canaliculus) physiology by waterborne cadmium: relationship to tissue bioaccumulation and effect of exposure duration[J]. Aquatic Toxicology, 2012, 124-125: 114/124. DOI: 10.1016/j.aquatox.2012.07.013.

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Combined Effects of Microplastics and Cadmium on Standard Metabolism of Cyprinus carpio var. Quanzhounensis

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