桂花精油在延缓衰老中的作用探究

doi:10.16088/j.issn.1001-6600.2024042903

Abstract

Abstract: This study explores the anti-aging effects of osmanthus essential oil on human neuroblastoma SH-SY5Y cells and an acute aging mouse model. In vitro experiments, the anti-aging effects of osmanthus essential oil was assessed by measuring its impact on the expression of aging markers and the levels of reactive oxygen species (ROS) in SH-SY5Y cells. The results indicate that osmanthus essential oil at concentrations of 2-100 μg/mL is non-toxic to SH-SY5Y cells and significantly reduces H₂O₂-induced ROS levels and improved aging cell characteristics, including reducing the expression of senescence-associated secretory phenotype (SASP) genes and aging-related genes (P16, P53, and P21), and decreasing SA-β-gal production. The vivo experiments showed that different doses of osmanthus essential oil (0.012 5, 0.025, and 0.05 μL/g) significantly increased antioxidant enzyme (SOD, CAT) activities in the serum of acutely aged mice, while reducing MDA and ROS levels. Combining the results in vitro and vivo experiments, osmanthus essential oil significantly delays the aging process in cellular and animal models through its potent antioxidant effects, providing strong scientific evidence for its application in anti-aging treatments.

Key words: Osmanthus fragrans, essential oil, anti-aging, antioxidant activity, animal models

CLC Number: TQ654.2

LU Xiaoqian, DO Qiuling, LI Anqi, WU Qiong. Study on Role of Osmanthus Essential Oil in Delaying Aging[J].Journal of Guangxi Normal University(Natural Science Edition), 2025, 43(1): 75-84.

References

[1] WANG H S, GAN D H, ZHANG X P, et al. Antioxidant capacity of the extracts from pulp of Osmanthus fragrans and its components[J]. LWT-Food Science and Technology, 2010, 43(2): 319-325. DOI: 10.1016/J.LWT.2009.08.003.
[2] LE D D, LEE Y E, LEE M. Triterpenoids from the leaves of Osmanthus fragrans var. aurantiacus with their anti-melanogenesis and anti-tyrosinase activities[J]. Natural Product Research, 2022, 36(24): 6414-6420. DOI: 10.1080/14786419.2022.2035384.
[3] LU S H, HUANG J, ZHOU Z B, et al. Chemical constituents from the branches of Osmanthus fragrans “Ziyingui” and their antioxidant activities and inhibitory effects on α-glucosidase and α-amylase[J]. Chemistry of Natural Compounds, 2023, 59(3): 535-536. DOI: 10.1007/s10600-023-04044-1.
[4] HUANG B, CHEN H Q, SHAO L Q. The ethanol extract of Osmanthus fragrans attenuates Porphyromonas gingivalis lipopolysaccharide-stimulated inflammatory effect through the nuclear factor erythroid 2-related factor-mediated antioxidant signalling pathway[J]. Archives of Oral Biology, 2015, 60(7): 1030-1038. DOI: 10.1016/j.archoralbio.2015.02.026.
[5] QIAN Y C, SHAN L M, ZHAO R, et al. Recent advances in flower color and fragrance of Osmanthus fragrans[J]. Forests, 2023, 14(7): 1403. DOI: 10.3390/f14071403.
[6] TANG P, WANG J Q, WANG Y F, et al. Comparison analysis of full-spectrum metabolomics revealed on the variation of potential metabolites of unscented, Chloranthus spicatus scented, and Osmanthus fragrans (Thunb.) Lour. scented Congou black teas[J]. Frontiers in Nutrition, 2023, 10: 1234807. DOI: 10.3389/fnut.2023.1234807.
[7] GANDHI S G, MAHAJAN V, BEDI Y S. Changing trends in biotechnology of secondary metabolism in medicinal and aromatic plants[J]. Planta, 2015, 241(2): 303-317. DOI: 10.1007/s00425-014-2232-x.
[8] YIN W, SONG Z R, LIU J Q, et al.[Chemical constituents of Osmanthus fragrans] [J]. China Journal of Chinese Materia Medica, 2015, 40(4): 679-685. DOI: 10.4268/cjcmm20150420.
[9] XIN H P, WU B H, ZHANG H H, et al. Characterization of volatile compounds in flowers from four groups of sweet osmanthus (Osmanthus fragrans) cultivars[J]. Canadian Journal of Plant Science, 2013, 93(5): 923-931. DOI: 10.4141/cjps2012-333.
[10] TAN D L. Negotiating cultural and linguistic differences in translation through transformation[J]. The Emily Dickinson Journal, 1997, 6(2): 50-55. DOI: 10.1353/edj.0.0054.
[11] 马晓莉,林文彬,张国林.野桂花化学成分研究[J].天然产物研究与开发,2009,21(4):593-599. DOI: 10.3969/j.issn.1001-6880.2009.04.013.
[12] 吴丹,罗世琼,杨占南,等.银桂花不同组织器官的挥发性化学成分[J].贵州农业科学,2015,43(1):120-122,125. DOI: 10.3969/j.issn.1001-3601.2015.01.030.
[13] KORNIENKO A, EVIDENTE A. Chemistry, biology, and medicinal potential of narciclasine and its congeners[J]. Chemical Reviews, 2008, 108(6): 1982-2014. DOI: 10.1021/cr078198u.
[14] FU C C, XU F Y, QIAN Y C, et al. Secondary metabolites of Osmanthus fragrans: metabolism and medicinal value[J]. Frontiers in Pharmacology, 2022, 13: 922204. DOI: 10.3389/fphar.2022.922204.
[15] CAI X, MAI R Z, ZOU J J, et al. Analysis of aroma-active compounds in three sweet osmanthus (Osmanthus fragrans) cultivars by GC-olfactometry and GC-MS[J]. Journal of Zhejiang University-Science B, 2014, 15(7): 638-648. DOI: 10.1631/jzus.B1400058.
[16] 邹晶晶,蔡璇,曾祥玲,等.桂花不同品种开花过程中香气活性物质的变化[J].园艺学报,2017,44(8):1517-1534. DOI: 10.16420/j.issn.0513-353x.2017-0050.
[17] 夏科,蒋柏生,赵志国,等.桂林地区不同桂花品种花香成分比较分析[J].广西植物,2018,38(11):1493-1504. DOI: 10.11931/guihaia.gxzw201803047.
[18] 施婷婷,杨秀莲,王良桂.‘波叶金桂’花香成分的释放规律[J].南京林业大学学报(自然科学版),2018,42(2):97-104. DOI: 10.3969/j.issn.1000-2006.201612033.
[19] 朱琳琳,周婉飞,宁璇,等.不同桂花品种游离态香气物质分析[J].园艺学报,2022,49(11):2395-2406. DOI: 10.16420/j.issn.0513-353x.2021-0773.
[20] 侯丹,付建新,张超,等.桂花品种‘堰虹桂’‘玉玲珑’和‘杭州黄’的香气成分及释放节律[J].浙江农林大学学报,2015,32(2):208-220. DOI: 10.11833/j.issn.2095-0756.2015.02.007.
[21] 俞雅芮,刘梦影,黄娇,等.金桂花中的一个新单萜化合物[J].药学学报,2022,57(4):1080-1084. DOI: 10.16438/j.0513-4870.2021-1418.
[22] LIU J, NAKAMURA S, XU B, et al. Chemical structures of constituents from the flowers of Osmanthus fragrans var. aurantiacus[J]. Journal of Natural Medicines, 2015, 69(1): 135-141. DOI: 10.1007/s11418-014-0869-1.
[23] SHIN M, LIU Q F, CHOI B, et al. Neuroprotective effects of limonene (+) against Aβ42-induced neurotoxicity in a Drosophila model of Alzheimer’s disease[J]. Biological and Pharmaceutical Bulletin, 2020, 43(3): 409-417. DOI: 10.1248/bpb.b19-00495.
[24] 包骐林,王丹,陆葵青,等.桂花黄酮类化合物体内外抗氧化活性的研究[J].食品研究与开发,2018,39(21):14-20. DOI: 10.3969/j.issn.1005-6521.2018.21.003.
[25] HUANG S K H, BUENO P R P, GARCIA P J B, et al. Antioxidant, anti-inflammatory and antiproliferative effects of Osmanthus fragrans (Thunb.) Lour. flower extracts[J]. Plants, 2023, 12(17): 3168. DOI: 10.3390/plants12173168.
[26] LEE S H, SEO S W, SONG S Y, et al. HPLC analysis and antioxidant evaluation of acteoside-rich Osmanthus fragrans extracts[J]. Journal of Food Quality, 2020, 2020: 8851285. DOI: 10.1155/2020/8851285.
[27] XIONG L N, MAO S Q, LU B Y, et al. Osmanthus fragrans flower extract and acteoside protect against D-galactose-induced aging in an ICR mouse model[J]. Journal of Medicinal Food, 2016, 19(1): 54-61. DOI: 10.1089/jmf.2015.3462.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	HE Jing, FENG Yuanliu, SHAO Jingwen. Research Progress on Multi-source Data Fusion Based on CiteSpace[J]. Journal of Guangxi Normal University(Natural Science Edition), 2024, 42(5): 13 -27 .
[2]	LIU Changping, SONG Shuxiang, JIANG Pinqun, CEN Mingcan. Differential Passive N-path Filter Based on Switched Capacitors[J]. Journal of Guangxi Normal University(Natural Science Edition), 2024, 42(5): 52 -60 .
[3]	WANG Shuying, LU Yuxiang, DONG Shutong, CHEN Mo, KANG Bingya, JIANG Zhanglan, SU Chengyuan. Research Progress on the Propagation Process and Control Technology of ARGs in Wastewater[J]. Journal of Guangxi Normal University(Natural Science Edition), 2024, 42(6): 1 -15 .
[4]	ZHONG Qiao, CHEN Shenglong, TANG Congcong. Hydrogel Technology for Microalgae Collection: Status Overview, Challenges and Development Analysis[J]. Journal of Guangxi Normal University(Natural Science Edition), 2024, 42(6): 16 -29 .
[5]	ZHAI Siqi, CAI Wenjun, ZHU Su, LI Hanlong, SONG Hailiang, YANG Xiaoli, YANG Yuli. Dynamic Relationship Between Reverse Solute Flux and Membrane Fouling in Forward Osmosis[J]. Journal of Guangxi Normal University(Natural Science Edition), 2024, 42(6): 30 -39 .
[6]	ZHENG Guoquan, QIN Yongli, WANG Chenxiang, GE Shijia, WEN Qianmin, JIANG Yongrong. Stepwise Precipitation of Heavy Metals from Acid Mine Drainage and Mineral Formation in Sulfate-Reducing Anaerobic Baffled Reactor System[J]. Journal of Guangxi Normal University(Natural Science Edition), 2024, 42(6): 40 -52 .
[7]	LIU Yang, ZHANG Yijie, ZHANG Yan, LI Ling, KONG Xiangming, LI Hong. Current Status and Trends of Algal Coagulation Elimination Technology in Drinking Water Treatment: a Visual Analysis Based on CiteSpace[J]. Journal of Guangxi Normal University(Natural Science Edition), 2024, 42(6): 53 -66 .
[8]	TIAN Sheng, CHEN Dong. A Joint Eco-driving Optimization Research for Connected Fuel Cell Hybrid Vehicle via Deep Reinforcement Learning[J]. Journal of Guangxi Normal University(Natural Science Edition), 2024, 42(6): 67 -80 .
[9]	CHEN Xiufeng, WANG Chengxin, ZHAO Fengyang, YANG Kai, GU Kexin. A Single Intersection Signal Control Method Based on Improved DQN Algorithm[J]. Journal of Guangxi Normal University(Natural Science Edition), 2024, 42(6): 81 -88 .
[10]	LI Xin, NING Jing. Online Assessment of Transient Stability in Power Systems Based on Spatiotemporal Feature Fusion[J]. Journal of Guangxi Normal University(Natural Science Edition), 2024, 42(6): 89 -100 .

Study on Role of Osmanthus Essential Oil in Delaying Aging

PDF (PC)

Abstract

Cite this article

share this article

References

Related Articles 6

Metrics

Comments

Recommended 10

[1]	LI Ling, TANG Wendi, CHEN Shouhuan, NING Yulong, QIN Jiangke. Study on Extraction Technology and Antioxidant Activity of Total Alkaloids from Corydalis saxicola Bunting [J]. Journal of Guangxi Normal University(Natural Science Edition), 2022, 40(6): 145-153.
[2]	CHEN Xu, LIANG Chengqin. Spectral Features, α-glucosidase Inhibitory Activities, Antioxidant Activities and Quantitative Determination of Mogroside ⅡA of Siraitia grosvenorii [J]. Journal of Guangxi Normal University(Natural Science Edition), 2019, 37(3): 132-141.
[3]	LIU Xi. Antioxidant and Antitumor Activities of Extracts from Taxus chinensis var. mairei [J]. Journal of Guangxi Normal University(Natural Science Edition), 2016, 34(4): 55-59.
[4]	DENG Ye-cheng, LI Rui-yu, YANG Lin-lin, ZHONG Hui, ZHOU Qiu-yan, QING Zhen. Insecticidal Activities and Chemical Constituents of Essential Oils from Alien Invasive Plants Solidago canadensis and Wedelia trilobata [J]. Journal of Guangxi Normal University(Natural Science Edition), 2014, 32(2): 122-129.
[5]	DENG Ye-cheng, NING Lei. Inhibitory Activities and Application of Four Plant Essential Oils [J]. Journal of Guangxi Normal University(Natural Science Edition), 2012, 30(3): 288-294.
[6]	YIN Wen-qing, DUAN Shao-qing, ZHANG Yan, ZENG Li, SONG Xin-ming. Antioxidant Activities of Different Solvents Extracts and Alkaloids of Uncaria rhynchophylla (Miq.)Jacks [J]. Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(1): 31-34.