Journal of Guangxi Normal University(Natural Science Edition) ›› 2024, Vol. 42 ›› Issue (2): 175-182.doi: 10.16088/j.issn.1001-6600.2023041801

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Study on the Active Substances of Endophytic Fungus SgG4 from Sarcandra glabra Against Plant Pathogenic Fungi

WEI Jianfen1,2, DENG Zhiyong1,2, DENG Yecheng1,2*, LUO Haiyu1,2, MENG Siyu1,2, XU Ziwei1,2, SHI Yao1,2, LIANG Wenken1,2   

  1. 1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
    2. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2023-04-18 Revised:2023-05-15 Published:2024-04-22

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Abstract: The antifungal activity of Trichoderma spirale strain SgG4 from Sarcandra glabra against plant pathogenic fungi was studied. Theantifungal activity of SgG4 fermentation products was determined by mycelial growth rate method. The active extract of the fermentation product was separated and purified by silica gel column chromatography combined with activity tracing. The structure of the active compound was identified by nuclear magnetic resonance spectrum and mass spectrometry. The result showed that the antifungal active substance mainly existed in the ethyl acetate extract of fermentation product, which had good inhibitory activity against 13 plant pathogenic fungi, with EC50 values ranging from 0.000 2 to 2.475 7 g/L, among which the inhibitory activity against Ceratocystis paradoxa was the best. An antifungal compound was isolated from the ethyl acetate extract of the fermentation product and identified as Trichoderma acid. At the mass concentration of 0.1 g/L, Trichoderma acid had inhibitory effects on 13 plant pathogenic fungi, among which, the inhibitory rate against Pestalotiopsis theae, Phytophthora parasitica, Ceratocystis paradoxa and Athelia rolfsii was 100%, and the inhibitory rate against the other 9 plant pathogenic fungi is 21.43%-85.71%. It can be seen that Trichoderma acid has good antifungal activity and wide antifungal spectrum, and has a good development and application prospect in microbial source fungicides.

Key words: Sarcandra glabra, endophytic fungi, Trichoderma spirale, antifungal activity, active substance

CLC Number:  S432.44;S482.2
[1] 廖宏娟, 张志斌, 江玉梅, 等. 球毛壳菌对植物病原真菌和根结线虫的生物防治潜力[J]. 天然产物研究与开发, 2022, 34(6): 1076-1089. DOI: 10.16333/j.1001-6880.2022.6.021.
[2] 王蕊, 王腾, 李二峰. 生防芽孢杆菌在植物病害领域的研究进展[J]. 天津农学院学报, 2021, 28(4): 71-77. DOI: 10.19640/j.cnki.jtau.2021.04.015.
[3] 李思瑶, 张杨, 车永胜. 植物内生真菌Talaromyces sp.的次级代谢产物研究[J]. 中国抗生素杂志, 2022, 47(9): 913-919. DOI:10.13461/j.cnki.cja.007389.
[4] TALEA S, SIDDIQUI B S, MAJID K, et al. Bioactive natural products from endophytic fungus Aspergillus nidulans associated with Nyctanthes arbor-tristis Linn.[J]. Records of Natural Products, 2021, 15(6): 608-612.DOI: 10.25135/rnp.251.21.02.1986.
[5] LIU G R, HUO R Y, ZHAI Y N, et al. New bioactive sesquiterpeniods from the plant endophytic fungus Pestalotiopsis theae [J]. Frontiers in Microbiology, 2021, 12: 641504. DOI: 10.3389/fmicb.2021.641504.
[6] 黄燕, 曾东强, 唐文伟, 等. 植物内生真菌生物活性成分研究进展[J]. 天然产物研究与开发, 2020, 32(8): 1419-1437.DOI: 10.16333/j.1001-6880.2020.8.019.
[7] 严冬, 曾为林, 陈肖学, 等. 樟叶越桔嫩枝内生真菌的植物病原菌拮抗活性[J]. 中南林业科技大学学报, 2021, 41(3): 64-71.DOI: 10.14067/j.cnki.1673-923x.2021.03.008.
[8] 陈旭, 雷帮星, 文庭池, 等. 一株马比木植物内生真菌Trichoderma sp.抗癌活性研究[J]. 时珍国医国药, 2017, 28(3): 522-525. DOI: 10.3969/j.issn.1008-0805.2017.03.004.
[9] 赵丹. 植物内生真菌代谢产物中抗氧化活性物质的研究[D]. 长春: 吉林大学, 2016.
[10] 贾力维, 王谦博, 张爽, 等. 药用植物内生真菌抗肿瘤代谢产物研究进展[J]. 中医药信息, 2019, 36(2): 103-108. DOI:10.19656/j.cnki.1002-2406.190058.
[11] 孙涛, 孙之潭, 胡美英, 等. 鱼藤属植物内生真菌的分离及杀虫活性研究[J]. 环境昆虫学报, 2015, 37(4): 785-789.DOI: 10.3969/j.issn.1674-0858.2015.04.13.
[12] 刘慧波, 陈玉婵, 李赛妮, 等. 巴戟天内生真菌Diaporthe lithocarpus的次级代谢产物及其细胞毒活性研究[J]. 中药材, 2020, 43(10): 2439-2444.DOI: 10.13863/j.issn1001-4454.2020.10.018.
[13] 冯楠楠, 李青青, 沐琦, 等. 1株红树内生真菌高粱附球菌Epicoccum sorghinum的代谢产物及抗菌活性[J]. 华南农业大学学报, 2022, 43(4): 77-81.DOI: 10.7671/j.issn.1001-411X.202112017.
[14] 徐国波, 王栋, 朱勤凤, 等. 头花蓼内生真菌Diaporthe sp.代谢产物中的抗菌活性成分研究[J]. 贵州医科大学学报, 2020, 45(9): 1020-1024. DOI: 10.19367/j.cnki.2096-8388.2020.09.006.
[15] 徐艳琴, 刘小丽, 黄小方, 等. 草珊瑚的研究现状与展望[J]. 中草药, 2011, 42(12): 2552-2559.DOI: 10.7501/j.issn.0253-2670.
[16] 段营辉, 戴毅, 高昊, 等. 草珊瑚的化学成分研究[J]. 中草药, 2010, 41(1): 29-32.DOI: 10.3321/j.issn:0253-2670.2005.01.006.
[17] 蒋伟哲, 孔晓龙, 黄仁彬, 等. 肿节风片的抗菌和抗炎作用研究[J]. 广西中医学院学报, 2000, 17(1): 50-52. DOI: 10.3969/j.issn.1008-7486.2000.01.032.
[18] 杨云成, 武文豪, 唐守春, 等. 草珊瑚不同提取物抗氧化活性比较研究[J]. 现代农业科技, 2022(17): 190-195.DOI: 10.3969/j.issn.1007-5739.2022.17.049.
[19] 李学农. 草珊瑚醇提物抗肿瘤有效部位的HPLC分离及各分离组分体外抗肿瘤作用的实验研究[J]. 海峡药学, 2013, 25(8): 184-185.DOI: 10.3969/j.issn.1006-3765.2013.08.086.
[20] 朱丽萍. 草珊瑚的保肝活性成分研究[D]. 北京: 中国协和医科大学, 2008.
[21] 陈芳有, 陈志超, 罗永明. 草珊瑚化学成分及生物活性研究进展[J]. 中国中药杂志, 2022, 47(4): 872-879. DOI: 10.19540/j.cnki.cjcmm.20211012.201.
[22] 顾福莉, 徐涑珅, 石昌国. 肿节风注射液辅助治疗老年重症肺炎的疗效及机制研究[J]. 中国现代医生, 2020, 58(2): 129-131, 150.
[23] 董媛, 张莉莉, 史丽娜, 等. 肿节风注射液辅助治疗晚期食道癌的疗效及对患者血清VEGF、S100A4水平的影响[J]. 现代生物医学进展, 2019, 19(19): 3712-3715, 3634.DOI: 10.13241/j.cnki.pmb.2019.19.026.
[24] 蓝松涛, 马留辉, 黄少军, 等. 草珊瑚林下有机栽培技术[J]. 现代农业科技, 2022(9): 41-44.DOI: 10.3969/j.issn.1007-5739.2022.09.013.
[25] 茅隆森. 桉树林下套种草珊瑚栽培技术研究[J]. 林业勘察设计, 2018, 38(1): 47-49.
[26] 蒙思妤. 草珊瑚内生真菌的抑菌活性及活性物质研究[D]. 桂林: 广西师范大学, 2022.
[27] 颜桢灵, 陈洁萍, 农小霞, 等. 柑橘内生真菌的分离鉴定及其发酵产物对柑橘溃疡病菌的抑制活性[J]. 广西植物, 2021, 41(7): 1196-1208.DOI: 10.11931/guihaia.gxzw202002021.
[28] DENG Y C, YU Y Z, LUO H Y, et al. Antimicrobial activity of extract and two alkaloids from traditional Chinese medicinal plant Stephania dielsiana[J]. Food Chemistry, 2011, 124(4): 1556-1560.DOI: 10.1016/j.foodchem.2010.08.011.
[29] 李冬利, 陈玉婵, 李义勇, 等. 1株白木香内生真菌中抗肿瘤活性成分的分离纯化和结构鉴定[J]. 微生物学杂志, 2010, 30(6): 1-5.DOI: 10.3969/j.issn.1005-7021.2010.06.001.
[30] 杨曦亮, 任梦瑶, 刘倩, 等. 近十年海洋来源木霉属真菌次生代谢产物研究进展[J]. 广西科学, 2021, 28(5): 440-450.DOI: 10.13656/j.cnki.gxkx.20211206.001.
[31] ZHAO D L, ZHANG X F, HUANG R H, et al. Antifungal nafuredin and epithiodiketopiperazine derivatives from the mangrove-derived fungus Trichoderma harzianum D13[J]. Frontiers in Microbiology, 2020, 11: 1495.DOI: 10.3389/fmicb.2020.01495.
[32] 邹积雪, 季乃云. 松节藻来源长枝木霉DL5-4化学成分及其生物活性研究[J]. 化学与生物工程, 2019, 36(3): 13-17.DOI: 10.3969/j.issn.1672-5425.2019.03.003.
[33] 解树涛, 宋晓妍, 石梅, 等. 康宁木霉(Trichoderma koningii)SMF2分泌的peptaibols类抗菌肽Trichokonins抑菌活性研究[J]. 山东大学学报(理学版), 2006, 41(6): 140-144.DOI: 10.3969/j.issn.1671-9352.2006.06.031.
[34] 钟慧, 钟勇, 卿朕, 等. 2种中药植物提取物抑菌活性初步研究[J]. 河南农业科学, 2015, 44(9): 64-68.DOI: 10.15933/j.cnki.1004-3268.2015.09.016.
[35] 王涛, 张献强, 林纬, 等. 罗汉果根腐病的室内药剂防治试验[J]. 广西植保, 2008, 21(4): 1-4.DOI: 10.3969/j.issn.1003-8779.2008.04.001.
[36] 李浩华, 李冬利, 章卫民. 木霉酸在制备抗真菌药物中的应用: CN201310016774[P]. 2013-05-08.
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