Abstract: In the peripheral blood leukocyte separation experiment, the red blood cells of tumor-bearing male mice with Lewis cancer cell (Lewis lung carcinoma, LLC) did not sink to the bottom but were stagnated in the middle layer,which was different from that of normal mice. To explore the further function of the Sdr9c7 gene and the possible mechanism of erythrocyte abnormality in tumor-bearing male mice, the expression of the Sdr9c7 gene was detected by real-time fluorescence quantitative PCR in red blood cells and lymphocyte isolated from the peripheral blood, and some tissues such as skin, liver, bone marrow, and blood vessels. Compared with the control group, the expression level of the Sdr9c7 gene in erythrocytes of tumor-bearing mice increased significantly, which was 2.60±0.44 times as that of normal mice. Although its expression showed no significant difference in skin and blood vessels, its expression in blood, bone marrow, liver, and lymphocyte of tumor-bearing mice were significantly down-regulated, which was 0.31±0.05, 0.44±0.04, 0.36±0.04 and 0.35±0.08 times as that of normal mice separately. The results suggested that the occurrence of erythrocyte abnormality in tumor-bearing mice might be related to the increased of Sdr9c7 gene expression. This result broadens the horizon for further study of the biological function of Sdr9c7 gene and provides a new perspective for the mechanism of erythrocyte abnormalities in the tumor-bearing state.

Key words: tumor-bearing mice, lung cancer, qRT-PCR, red blood cells, tissue expression