广西师范大学学报（自然科学版） ›› 2016, Vol. 34 ›› Issue (3): 131-137.doi: 10.16088/j.issn.1001-6600.2016.03.019

• • 上一篇    下一篇

沙田柚WRKY转录因子的克隆与序列分析

张渝1,2, 刘玉洁1,2, 郭丹妮1,2, 覃信梅1,2, 韩愈1,2, 李惠敏1,2, 秦新民1,2   

  1. 1.广西师范大学生命科学学院,广西桂林541006;
    2.广西师范大学珍稀濒危动植物生态与环境保护教育部重点实验室,广西桂林541006
  • 收稿日期:2015-11-27 出版日期:2016-09-30 发布日期:2018-09-17
  • 通讯作者: 秦新民(1956—),男,广西灵川人,广西师范大学教授,博士。E-mail:xmqin@mailbox.gxnu.edu.cn
  • 基金资助:
    国家自然科学基金资助项目(31360477);广西教育厅项目基金资助(2013YB036)

Cloning and Sequence Analysis of WRKY Transcription Factorfrom Citrus grandis var. Shatinyu Hort

ZHANG Yu1,2, LIU Yujie1,2, GUO Danni1,2, QIN Xinmei1,2, HAN Yu1,2, LI Huimin1,2, QIN Xinmin1,2   

  1. 1.College of Life Science, Guangxi Normal University, Guilin Guangxi 541006,China;
    2.Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection(Ministry of Education),Guangxi Normal University, Guilin Guangxi 541006,China
  • Received:2015-11-27 Online:2016-09-30 Published:2018-09-17

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摘要： 利用高通量测序技术对沙田柚自交和异交花柱进行转录组测序,差异分析得到沙田柚WRKY转录因子的序列。该基因的全长序列为1 178 bp (GenBank accession No. KU173833),含有993 bp的开放阅读框(ORF)可编码330个氨基酸,编码蛋白的相对分子质量为37.00 ku,理论等电点为5.79。与未授粉的花柱相比,沙田柚异花授粉1、2、3 d花柱中WRKY转录因子基因的表达量(RPKM)分别为5.67、26.04、17.08;而自花授粉1、2、3 d花柱中WRKY转录因子基因的表达量(RPKM)分别为15.67、14.96、3.89。氨基酸序列分析表明,该氨基酸序列与甜橙、金桔的同源性分别为98%、97%。系统进化分析发现沙田柚WRKY转录因子与甜橙、金桔的亲缘关系很近,属于一个分支。

关键词: 沙田柚, WRKY, 转录因子, 自交不亲和性

Abstract: In this study, the transcriptome of the self-pollinated styles and cross-pollinated styles of Citrus grandis var. Shatinyu Hort were sequenced by high-throughput sequencing technology. WRKY transcription factor gene of Citrus grandis var. Shatinyu Hort was obtained through variance analysis method. It is 1 178 bp in length with an open reading frame (ORF) of 993 bp, encoding 330 amino acids with deduced molecular weight of 37.00 ku, and theoretical pI value of 5.79. Compared with un-pollinated styles, the expression (RPKM) of WRKY transcription factor gene is respectively 5.67, 26.04 and 17.08 in 1, 2, 3 days cross-pollinated styles, and expression (RPKM) of the gene is respectively 15.67, 14.96 and 3.89 in 1, 2, 3 days self-pollinated styles. The homology analysis of amino acid sequence indicated that the WRKY transcription factor protein shared higher homology with that of Citrus sinensis (98%) and Citrus japonica (97%). Phylogenetic analysis revealed that WRKY transcription factor gene showed closer kinship with that of Citrus sinensis and Citrus_japonica,indicating that they belong to the same evolutionary branch.

Key words: Citrus grandis var. Shatinyu Hort, WRKY, transcription factor, self-incompatibility

中图分类号: 

  • Q946
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[1] 秦新民, 张渝, 刘玉洁, 郭丹妮, 李惠敏. 沙田柚S-RNase基因的克隆及序列分析[J]. 广西师范大学学报（自然科学版）, 2015, 33(1): 139-145.
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