广西师范大学学报（自然科学版） ›› 2016, Vol. 34 ›› Issue (1): 144-149.doi: 10.16088/j.issn.1001-6600.2016.01.023

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饥饿再投喂对鳜肌FSRP-1、FSRP-3和肠道PepT1基因表达的影响

汪建华1,2, 李玉珑2, 陈敦学1, 朱鑫2, 刘知行2, 张建社2, 褚武英2, 宾石玉1   

  1. 1.广西师范大学生命科学学院,广西桂林541006;
    2.长沙大学生物与环境工程系,湖南长沙410003
  • 收稿日期:2015-07-25 发布日期:2018-09-14
  • 通讯作者: 宾石玉(1963—),男,广西桂林人,广西师范大学教授,博士。E-mail:binsy@gxnu.edu.cn
  • 基金资助:
    国家自然科学基金资助项目(31472256,31560718,31230076);湖南省教育厅开放基金资助项目(15K011)

Effect of Starvating and Refeeding on Relative Expression of FSRP-1,FSRP-3 and PepT1 Gene in Mandarin Fish (Siniperca chuatsi)

WANG Jianhua1,2, LI Yulong2, CHEN Dunxue1, ZHU Xin2, LIU Zhixing2, ZHANG Jianshe2, CHU Wuying2, BIN Shiyu1   

  1. 1.College of Life Science,Guangxi Normal University,Guilin Guangxi 541006,China; 2. Department of Bioengineering and Environmental Science, Changsha University, Changsha Hunan 410003, China
  • Received:2015-07-25 Published:2018-09-14

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摘要： 为探讨饥饿再投喂对鳜肌FSRP-1、FSRP-3和肠道中PepT1基因表达的影响,本研究采用qRT-PCR技术,测定饥饿7 d再饱食一餐后0、1、3、6、12、24、48、96 h条件下鳜肌中FSRP-1、FSRP-3和肠道中PepT1基因表达水平的变化,研究结果表明:FSRP-1基因表达在投喂后12 h内显著升高(P<0.05),12-48 h维持在较高表达水平,48 h后显著降低(P<0.05);FSRP-3基因表达在投喂后1 h内显著降低(P<0.05),12 h达到峰值且表达量是1 h的5倍,48 h后显著降低(P<0.05);PepT1基因表达在投喂后6 h内显著降低(P<0.05),48 h时表达量最高。FSRP-1、FSRP-3和PepT1是与鱼类生长相关的重要基因,受饥饿再投喂的影响较大。

关键词: 鳜, 饥饿再投喂, 卵泡抑素相关蛋白1和3, 肠道小肽转运载体, 基因表达

Abstract: To investigate postprandial changes in transcript abundance following a single satiating meal in mandarin fish (Siniperca chuatsi) going on a fast for 1 week. The expression of FSRP-1 and FSRP-3 gene in fast myotomal muscle, as well as the PepT1 gene in small intestine were determined using quantitative real-time PCR at the following postprandial time points: 0, 1, 3, 6, 12, 24, 48 and 96 h. The results indicated that: FSRP-1 transcripts were sharply up-regulated within 12 h of refeeding (P<0.05), the transcripts were maintained in the high level from 12 h to 48 h, then it was significant down-regulation in response to feeding; The FSRP-3 gene expression was increased significantly (P<0.05) in 1 h postprandially, and reached peak expression levels at 12 h postprandially, and decreased significantly after 48 h postprandially (P<0.05). The expression level of FSRP-3 at 12 h was 5 times higher compared with which at 1 h; The PepT1 gene had decreased expression in 6 h after the meal administration significantly (P<0.05), and reached maximal expression levels at 48 h postprandially. FSRP-1, FSRP-3 and PepT1 were absolutely necessary, associated with growth, which were greatly influence by starvation and refeeding.

Key words: Siniperca chuatsi, starvation and refeeding, FSRP-1 and FSRP-3, PepT1, gene expression

中图分类号: 

  • S965.127
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