广西师范大学学报（自然科学版） ›› 2026, Vol. 44 ›› Issue (3): 148-162.doi: 10.16088/j.issn.1001-6600.2025061301

• 分子生物学与生物技术 • 上一篇    下一篇

贝莱斯芽孢杆菌 YWC-01全基因组测序及生物学特性分析

雷滢1, 许博文1, 秦海雄1, 黄心怡1, 赵甲元1, 杜娟2,3*   

  1. 1.四川师范大学 生命科学学院,四川 成都 610101;
    2.四川师范大学 地理与资源科学学院,四川 成都 610101;
    3.西南土地资源评价与监测教育部重点实验室(四川师范大学),四川 成都 610066
  • 收稿日期:2025-06-13 修回日期:2025-08-09 出版日期:2026-05-05 发布日期:2026-05-13
  • 通讯作者: 杜娟(1972—),女,四川雷波人,四川师范大学高级实验师。E-mail: dujuan@sicnu.edu.cn
  • 基金资助:
    国家自然科学基金青年科学基金(31801644)

Whole Genome Sequencing and Biological Characterization of Bacillus velezensis YWC-01

LEI Ying1, XU Bowen1, QIN Haixiong1, HUANG Xinyi1, ZHAO Jiayuan1, DU Juan2,3*   

  1. 1. College of Life Science, Sichuan Normal University, Chengdu Sichuan 610101, China;
    2. Faculty Geography Resources Science, Sichuan Normal University, Chengdu Sichuan 610101, China;
    3. Key Laboratory of Land Resources Evalution and Monitoring in Southwest (Sichuan Normal University), Ministry of Education, Chengdu Sichuan 610066, China
  • Received:2025-06-13 Revised:2025-08-09 Online:2026-05-05 Published:2026-05-13

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摘要： 本文基于全基因组测序和生物学特性,评估1株从大曲中筛选到的可生物降解呕吐毒素(deoxynivalenol, DON)的贝莱斯芽孢杆菌YWC-01的益生潜力。采用稀释平板划线分离对大曲进行初筛、复筛并对获取的菌株进行鉴定。采用Illumina NovaSeq和PacBio Sequel测序平台完成全基因组测序。通过高效液相色谱检测该菌株降解DON的能力,并通过多数据库进行基因组功能注释。针对其代谢特征,预测碳水化合物活性酶家族组成和次级代谢产物基因簇,并进行毒力因子分析与耐药分子机制评估,同时基于基因gyrA构建分子进化树,揭示菌株进化关系。结果:筛选得到1株可以高效降解DON的贝莱斯芽孢杆菌YWC-01。在DON浓度为140 mg/L时,YWC-01对DON的降解率最大(29.1%);YWC-01菌株全基因组大小为4.04 Mb,G+C平均含量为46.51%,共编码3 928个基因,含87个tRNA基因,131个CAZy家族基因;antiSMASH预测显示YWC-01基因组含有macrolactin H、bacillaene和fengycin等13个次级代谢产物基因簇。综上,YWC-01致病性有限且可能产生多种功能性代谢产物,具有作为微生态制剂的潜力。

关键词: 贝莱斯芽孢杆菌, 脱氧雪腐镰刀菌烯醇, 全基因组测序, 生物学特性, 安全性

Abstract: This study characterizes Bacillus velezensis YWC-01, a strain isolated from Daqu with biodegradative activity against deoxynivalenol (DON), and evaluates its probiotic potential through whole-genome sequencing and biological trait analysis. Primary and secondary screening via dilution-plate streaking isolated the strain from Daqu, followed by identification. Whole-genome sequencing employed Illumina NovaSeq and PacBio Sequel platforms. DON degradation efficiency was quantified using high-performance liquid chromatography (HPLC). Genomic functional annotation utilized multiple databases. Metabolic traits were assessed by predicting carbohydrate-active enzyme (CAZy) families and secondary metabolite gene clusters, alongside virulence factor screening and antibiotic resistance mechanism evaluation. Phylogenetic analysis based on gyrA gene elucidated evolutionary relationships. The results showed that, YWC-01 degraded DON at a maximum rate of 29.1% (initial concentration: 140 mg/L). Its genome spanned 4.04 Mb with 46.51% G+C content, encoding 3 928 genes (including 87 tRNAs and 131 CAZy families). AntiSMASH predicted 13 secondary metabolite gene clusters, including those for macrolactin H, bacillaene, and fengycin synthesis. YWC-01 exhibits limited pathogenicity and harbors genetic potential for diverse functional metabolites, supporting its utility as a probiotic agent.

Key words: Bacillus velezensis, deoxynivalenol, whole genome sequencing, biological characteristics, safety

中图分类号:  Q933; TS201.3

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