广西师范大学学报（自然科学版） ›› 2023, Vol. 41 ›› Issue (1): 155-163.doi: 10.16088/j.issn.1001-6600.2021102801

• 研究论文 • 上一篇    下一篇

柑橘内生真菌LJZ-Y-11次生代谢产物抑菌活性研究

李奇聪1,2,3,4, 陈洁萍1,2,3,4, 欧艳绍1,2,3,4, 杨树贤1,2,3,4, 邓志勇1,2,3,4, 骆海玉1,2,3,4*, 邓业成1,2,3,4*   

  1. 1.珍稀濒危动植物生态与环境保护教育部重点实验室(广西师范大学), 广西 桂林 541006;
    2.广西漓江流域景观资源保育与可持续利用重点实验室(广西师范大学), 广西 桂林 541006;
    3.广西珍稀濒危动物生态学重点实验室(广西师范大学), 广西 桂林 541006;
    4.广西师范大学可持续发展创新研究院, 广西 桂林 541006
  • 收稿日期:2021-10-28 修回日期:2021-12-04 出版日期:2023-01-25 发布日期:2023-03-07
  • 通讯作者: 骆海玉(1985—),女,广西桂林人,广西师范大学讲师,博士。E-mail:luohaiyu69@163.com; 邓业成(1965—),男,广西全州人,广西师范大学教授,博士。E-mail:dyecheng@163.com
  • 基金资助:
    广西自然科学基金(2018GXNSFAA281013); 广西重点研发计划(桂科AB21196065); 广西创新驱动发展专项(桂科AA20161002-1); 桂林市科学研究与技术开发计划(20190205); 广西师范大学珍稀濒危动植物生态与环境保护教育部重点实验室主任基金(ERESEP2021Z05)

Antimicrobial Activity of the Secondary Metabolites from Citrus Endophytic Fungus Nemania sp. LJZ-Y-11

LI Qicong1,2,3,4, CHEN Jieping1,2,3,4, OU Yanshao1,2,3,4, YANG Shuxian1,2,3,4, DENG Zhiyong1,2,3,4, LUO Haiyu1,2,3,4 *, DENG Yecheng1,2,3,4*   

  1. 1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
    2. Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), Guilin Guangxi 541006, China;
    3. Guangxi Key Laboratory of Rare and Endangered Animal Ecology, Guilin Guangxi 541006, China;
    4. Institute for Sustainable Development and Innovation (Guangxi Normal University), Guilin Guangxi 541006, China
  • Received:2021-10-28 Revised:2021-12-04 Online:2023-01-25 Published:2023-03-07

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摘要： 本文选取柑橘内生真菌Nemania sp. LJZ-Y-11进行发酵培养,提取制备其乙酸乙酯提取物并测定抑菌活性,旨在为其开发利用奠定基础。结果表明,其乙酸乙酯提取物对9种植物病原真菌和10种动物病原菌均具有显著抑制作用。处理浓度为2.5 g/L时,对9种植物病原真菌的抑制活性均超过50%,除对贡柑链格孢菌的抑制率为56.52%外,对其余8种病原菌的抑菌率均为100%,EC50值在0.004 8~0.154 4 g/L。其中,对玉米大斑病菌、金橘砂皮病菌和烟草黑胫病菌抑菌活性最为显著,EC50值分别为0.004 8、0.005 3和0.006 7 g/L,均小于0.01 g/L;其次为辣椒炭疽病菌、茶轮斑病菌和甘蔗凤梨病菌,EC50值分别为0.022 7、0.035 6和0.067 9 g/L,均小于0.1 g/L;对甘蓝黑斑病菌和水稻胡麻叶斑病菌也有较好的抑制活性,EC50值分别为0.101 3和0.154 4 g/L。对10种动物病原菌均具有不同程度的抑制效果,MIC值为0.078 1~2.500 0 g/L。其中,对枯草芽孢杆菌、大肠杆菌、金黄色葡萄球菌、蜡样芽孢杆菌、炭疽杆菌、白色念珠菌具有较强的抑菌活性,MIC值均小于0.2 g/L,其中对枯草芽孢杆菌的抑菌活性最强,MIC值为0.078 1 g/L;对巨大芽孢杆菌和溶壁微球菌也有较好的抑菌活性,MIC值均为0.625 g/L;对产气肠杆菌和伤寒沙门氏菌的抑制活性相对较弱,MIC值仅为2.5 g/L。本文报道的柑橘内生真菌Nemania sp. LJZ-Y-11代谢产物具有广谱、显著抑菌活性,研究结果可为其次生代谢产物作为生物源抗菌剂开发利用提供依据。

关键词: 柑橘内生真菌, Nemania sp., 乙酸乙酯提取物, 抑菌活性

Abstract: Based on previous studies, citrus endophytic fungus Nemania sp. LJZ-Y-11 was selected for fermentation, extract preparation and evaluation of its antimicrobial activity, aiming to lay a foundation for the development and utilization of its metabolites. The antifungal and antibacterial activities of the ethyl acetate extract were determined by using a mycelium growth rate method and toxic medium coating method, respectively. The results showed that the ethyl acetate extract of LJZ-Y-11 exhibited an excellent antifungal activity against most of tested plant pathogenic fungi, and had an antibacterial activity against 10 animal pathogens to some extent. The inhibitory rate against 9 plant pathogenic fungi were all more than 50% at 2.5 g/L of the extract. Moreover, except that the inhibitory rate against Alternaria citri was 56.52%, the others were 100%. The toxicity of extract against 8 plant pathogenic fungi was determined furtherly, with EC50 values ranging from 0.004 8 g/L to 0.154 4 g/L. There was the highest inhibitory activity against Exserohilum turcicum, Diaporthe citri and Phytophthora parasitica var. nicotianae with EC50 values of 0.004 8, 0.005 3 and 0.006 7 g/L, respectively, less than 0.01 g/L, following by Colletotrichum capsici, Pestalotiopsis theae and Ceratocystis paradoxa with EC50 values of 0.022 7, 0.035 6 and 0.067 9 g/L, respectively, less than 0.1 g/L; and also had a significant inhibitory activity against Alternaria oleracea and Cochliobolus miyabeanus with EC50 values of 0.101 3 g/L and 0.154 4 g/L, respectively. In addition, the extract also showed significant antibacterial activities against 10 animal pathogens with MIC values of 0.078 1-2.5 g/L. There were high inhibitory activities against Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Bacillus cereus, Bacillus anthraci and Monilia albican with MIC values of less than 0.2 g/L, and had the highest inhibitory activity against Bacillus subtilis with a MIC value 0.078 1 g/L. There were also strong inhibitory effects against Bacillus megaterium and Micrococcus lysodeikticus, with a MIC value of 0.625 g/L. The inhibition activity against Enterobacter aerogenes and Salmonella typhi was relatively weaker, with MIC values of 2.5 g/L. The results in this study reveal that the secondary metabolites of LJZ-Y-11 has broad-spectrum and significant antimicrobial activity against the common pathogens, providing a useful experiment basis for the development and utilization of the secondary metabolites as biological antimicrobial agents.

Key words: citrus endophytic fungi, Nemania sp. ethyl acetate extract, antimicrobial activity

中图分类号: 

  • TQ455
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