广西师范大学学报(自然科学版) ›› 2012, Vol. 30 ›› Issue (3): 269-275.

• • 上一篇    下一篇

红腹锦鸡线粒体基因组全序列及其结构分析

秦新民, 李惠敏, 覃屏生, 曾振华, 石菁萍, 管清新, 曾德龙   

  1. 广西师范大学生命科学学院,广西桂林541004
  • 收稿日期:2012-03-14 出版日期:2012-09-20 发布日期:2018-12-04
  • 通讯作者: 秦新民(1956—),男,广西灵川人,广西师范大学教授,博士。E-mail:xmqin@gxnu.edu.cn
  • 作者简介:秦新民,广西灵川人,教授,博士,硕士研究生导师。
  • 基金资助:
    国家自然科学基金资助项目(31160071);广西壮族自治区研究生教育创新项目(2010106020710M56)

Analysis of the Characteristics of Mitochondrial Genomes in Chrysolophus pictus

QIN Xin-min, LI Hui-min, QIN Ping-sheng, ZENG Zhen-hua, SHI Jing-ping, GUAN Qing-xin, ZENG De-long   

  1. College of Life Science,Guangxi Normal University,Guilin Guangxi 541004,China
  • Received:2012-03-14 Online:2012-09-20 Published:2018-12-04

摘要: 采用PCR扩增方法测定红腹锦鸡Chrysolophus pictus线粒体基因组全序列:序列全长16 678 bp,共有13个蛋白质编码基因、2个rRNA基因和22个tRNA基因。基因组的组成、顺序、编码链的选择、tRNA的结构都与绝大多数鸟类相同或相近。红腹锦鸡线粒体基因组碱基组成分别为:A(30.4%)、T(24.8%)、C(31.2%)、G(13.6%)。总的A+T含量为55.2%。除了COⅠ基因起始密码子是GTG,其余12个蛋白质编码基因的起始密码子都是ATG。tRNA基因核苷酸长度为65~76 nt,12S rRNA 和16S rRNA基因分别为968和1 604 nt。

关键词: 红腹锦鸡, 雉科, 线粒体基因组

Abstract: The complete mitochondrial genome of Chrysolophus pictus was sequenced by PCR amplification.The entire mtDNA sequence was 16 678bp in length and contained 13 protein-coding genes,2 rRNA genes,22 tRNA genes,anda control region (D-loop).Its gene arrangement pattern was identical with thoseof other avian.The average nucleotide composition was:30.4% of A,24.8% of T,31.2% of C,13.6% of G.All protein-coding genes initiated with ATG,except for COI,which began with GTG.Transfer RNA genes ranged in length from 65 to 76 nt.The12S rRNA and 16S rRNA genes in C.pictus were 968 and 1 604 nucleotides long.

Key words: Chrysolophus pictus, Phasianidae, mitochondrialgenome

中图分类号: 

  • Q755
[1] 郑作新.中国动物志:鸟纲:第四卷:鸡形目[M].北京:科学出版社,1978:179-182.
[2] PETERS J L.Check-List of birds of the world[M].Cambridge Harvard Uruversity Press,1934:401.
[3] DELACOUR J.The Pheasants of the world[M].London,Country Life Limited,1957.
[4] 杨岚.中国雉类:白腹锦鸡[M].北京:中国林业出版社,1992:141.
[5] 陈玉琴,俞诗源.红腹锦鸡、石鸡和雉鸡的部分血液生理生化指标[J].动物学报,2007,53(4):674-681.
[6] 张亚平,陈欣,施立明.两种锦鸡和环颈雉线粒体DNA(mtDNA)的比较研究[J].动物学研究,1991,12(4):387-392.
[7] 向余劲攻,杨岚,张亚平.白腹锦鸡和红腹锦鸡的遗传分化[J].遗传,2000,22(4):225-228.
[8] 黄族豪,龙进,张立勋,等.从线粒体DNA控制区基因探讨红腹锦鸡和白腹锦鸡的分类关系[J].江西师范大学学报:自然科学版,2006,30(1):91-94.
[9] ANDERSON S,BANKIER A,BARRELL B G,et al.Sequence and organization of the human mitochondrial genome[J].Nature,1981,290:457-465.
[10] HARRISON R G.Animal mitochondrial DNA as a genetic marker in population and evolutionary biology[J].Trends in Ecology and Evolution,1989,4(1):6-11.
[11] JOSHUA S R,JENNIFER C A,CHRISTOPHER C A,et al.Molecular systematics of primary reptilian lineages and the tuatara mitochondrial genome[J].Molecular Phylogenetics and Evolution,2003,29(2):289-297.
[12] BOORE J L.Complete mitochondrial genome sequence of Urechis caupo,a representative of the phylum Echiura[J].BMC Genomics,2004,5(1):67.
[13] PARHAM J F,FELDMAN C R,BOORE J L.The complete mitochondrial genome of the enigmatic bigheaded turtle (Platysternon):description of unusual genomic features and the reconciliation of phylogenetic hypotheses based on mitochondrial and nuclear DNA[J].BMC Evolutionary Biology,2006,6:11-25.
[14] SHEN Yong-yi,LIANG Lu,SUN Yan-bo,et al.A mitogenomic perspective on the ancient,rapid radiation in the Galliformes with an emphasis on the Phasianidae[J].BMC Evolutionary Biology,2010,10:132-141.
[15] 汪永庆,王新国,徐来祥,等.一种动物基因组DNA提取方法的改进[J].动物学杂志,2001,36(1):27-29.
[16] MINDELL D P,SORENSON M D,DIMCHEFF D E,et al.Interordinal relationships of birds and other reptiles based on whole mitochondrial genomes[J].Systematic Biology,1999,48(1):138-152.
[17] CROWE T M,BOWIE R C K,BLOOMER P,et al.Phylogenetics,biogeographyand classification of,and character evolution in,gamebirds (Aves:Galliformes):effects of character exclusion,data partitioning and missing data[J].Cladistics,2006,22(6):495-532.
[18] THOMPSON J D,GIBSON T J,PLEWNIAK F,et al.The CLUSTALX windows interface:flexible strategies for multiple sequence aligrunent aided by quality analysis tools[J].Nucleic Acid Research,1997,24:4876-4882.
[19] LOWE T M,Eddy S R.tRNAscan-SE:a program for improved detection of transfer RNA genes in genomic sequence[J].Nucleic Acids Research,1997,25(5):955-964.
[20] DESJARDINS P,MORAIS R.Sequence and gene organization of the chicken mitochondrial genome:A novel gene order in higher vertebrates[J].Journal ofMolecular Biology,1990,212:599-634.
[21] JANKE A,ARNASON U.The complete mitochondrial genome of Alligatormississippiensis and the separation between recent archosauria (birds and crocodiles)[J].Molecular Biology and Evolution,1997,14(12):1266-1272.
[22] QUINN T W,WILSON A C.Sequence evolution in and around the mitochondrial control region in birds[J].Journal of Molecular Evolution,1993,37:417-425.
[23] SBISA E,TANZARIELLO F,REYES A,et al.Mammalian mitochondrial D-loop region structural analysis:identification of new conserved sequences and their functional and evolutionary implications[J].Gene,1997,205(1/2):125-140.
[24] RUOKONEN M,KVIST L.Structure and evolution of the avian mitochondrial control region[J].Molecular Phylogenetics and Evolution,2002,23(3):422-432.
[25] 黄族豪,廖信军.红腹锦鸡线粒体DNA控制区结构和遗传变异研究[J].生命科学研究,2011,15(3):223-228.
[1] 杨丹丹, 谢永广, 宾石玉, 罗华辉, 安苗, 陈敦学. 波纹鳜线粒体基因克隆及系统发育信息分析[J]. 广西师范大学学报(自然科学版), 2019, 37(4): 119-126.
[2] 覃屏生, 石菁萍, 管清新, 曾德龙, 秦新民. 蛇雕线粒体DNA全序列测定与分析[J]. 广西师范大学学报(自然科学版), 2013, 31(4): 115-120.
[3] 陈敦学, 李玉珑, 宾石玉, 农小献, 刘希良, 成嘉, 张建社, 褚武英. 翘嘴鳜线粒体基因组全序列的克隆与特征分析[J]. 广西师范大学学报(自然科学版), 2011, 29(4): 111-116.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 广西师范大学学报(自然科学版)编辑部
地址:广西桂林市三里店育才路15号 邮编:541004
电话:0773-5857325 E-mail: gxsdzkb@mailbox.gxnu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发