广西师范大学学报（自然科学版） ›› 2017, Vol. 35 ›› Issue (3): 111-118.doi: 10.16088/j.issn.1001-6600.2017.03.014

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氢化物发生-罗丹明6G荧光分光光谱法测定痕量As

李重宁1, 2, 杨铎2, 潘宏程1*, 温桂清2, 梁爱惠2, 蒋治良2*   

  1. 1.桂林理工大学化学与生物工程学院,广西桂林541006;
    2. 广西师范大学环境与资源学院,广西桂林541004
  • 出版日期:2017-07-25 发布日期:2018-07-25
  • 通讯作者: 蒋治良(1965—),男,广西全州人,广西师范大学教授,博导。E-mail: zljiang@mailbox.gxnu.edu.cn
    潘宏程(1975—),男,广西平乐人,桂林理工大学教授,博士。E-mail:hcpan@163.com
  • 基金资助:
    国家自然科学基金(21367005,21465006,21477025,21667006);广西自然科学基金(2013GXNSFFA019003)

Hydride Generation-Rhodamine 6G Fluorescence Method forthe Determination of Trace Arsenic

LI Chongning1,2, YANG Duo2, PAN Hongcheng1*, WEN Guiqing2, LIANG Aihui2, JIANG Zhiliang2*   

  1. 1. College of Chemistry and Bioengineering, Guilin University of Technology, Guilin Guangxi 541006;
    2. College ofEnvironment and Resource, Guangxi Normal University, Guilin Guangxi 541004, China
  • Online:2017-07-25 Published:2018-07-25

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摘要： 本文建立了以Rh6G-I-3体系荧光光谱法测量痕量As的方法。在硫酸介质中,以硼氢化钠(NaBH4)为还原剂,可将As还原为砷化氢(AsH3)气体,该气体可还原I-3。当I-3与罗丹明6G(Rh6G)形成缔合物微粒时,体系在562 nm处的荧光信号较弱,随着I-3被AsH3被还原,体系中游离的Rh6G浓度增大,在562 nm处的荧光信号增强。在选定条件下,As浓度在0.011～0.997 mg/L与体系荧光增强值ΔF562呈线性关系,线性回归方程为ΔF562=825.1c-26.8,检出限为6.28 μg/L。据此建立一个检测As的荧光光谱法。

关键词: 砷, 氢化物发生, 罗丹明6G, 荧光法

Abstract: This paper introduces a new fluorescence method for the detection of trace arsenic in the Rh6G-I3 system. In sulfuric acid medium, arsenic is reduced by NaBH4 to form AsH3 which can reduce I-3 into I-. With I-3 conjugating with rhodamine 6G (Rh6G) to form Rh6G-I3 associated particles, the fluorescence intensity of Rh6G becomes weak at 562 nm. As I-3 ion is reduced by AsH3 resulting in the concentration increase of free Rh6G, its fluorescence intensity increases. Under the selected conditions, the increasing values (ΔF562) of the fluorescence intensity at 562 nm is linear in the range of 0.011～0.997 mg/L As, with the regression equation of ΔF = 825.1c-26.8 and a detection limit of 6.28 μg/L. Thus, a new fluorescence method for the detection of As was set up.

Key words: arsenic, hydride generation, rhodamine 6G, fluorescence

中图分类号: 

  • O657.3
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