广西师范大学学报（自然科学版） ›› 2013, Vol. 31 ›› Issue (1): 67-71.

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白花丹素衍生物Pln-7-co(oAc)体外抗肿瘤作用研究

彭艳1,2, 蔡乐靖1, 周远华1, 黎智1, 刘仁路1, 仲辉2, 张国海2   

  1. 1.广西师范大学生命科学学院,广西桂林541004;
    2.广西师范大学化学化工学院,广西桂林541004
  • 收稿日期:2012-07-06 出版日期:2013-03-20 发布日期:2018-11-26
  • 通讯作者: 张国海(1984—),男,山东聊城人,广西师范大学教师。E-mail:zhangguohaiwansui@126.com
  • 基金资助:
    973计划前期研究专项(2011CB512005);广西自然科学基金研究生创新项目(2011106020710M55);药用资源化学与药物分子工程教育部重点实验室课题(CMEMR2012-A12,CMEMR2012-A15)

In vitro Anti-tumor Effect of Plumbagin Derivative Pln-7-co(oAc)

PENG Yan1,2, CAI Le-jing1, ZHOU Yuan-hua1, LI Zhi1, LIURen-lu1, ZHONG Hui2, ZHANG Guo-hai2   

  1. 1.College of Life Science,Guangxi Normal University,Guilin Guangxi 541004,China;
    2.College of Chemistry and Chemical Engineering,Guangxi Normal University,Guilin Guangxi 541004,China
  • Received:2012-07-06 Online:2013-03-20 Published:2018-11-26

摘要： 本文以人肺癌NCI-H460、A549,肝癌BEL-7404,胃癌MCG-803细胞株以及正常肝细胞HL-7702作为受试细胞,分别采用MTT比色法、qPCR技术检测白花丹素衍生物(Pln-7-co(oAc))对细胞增殖的抑制作用以及对抑癌基因LRRC3B mRNA表达量的影响。结果显示,在10 μmol/L药物的作用下Pln-7-co(oAc)对NCI-H460细胞增殖的抑制率达到86.68%±2.89%,明显高于A549(59.56%±9.00%),肝癌BEL-7404(-7.24%±2.93%),胃癌MCG-803(15.27%±5.87%)。在IC50测试实验中,Pln-7-co(oAc)对NCI-H460细胞株的增殖抑制作用呈现很好的剂量依赖关系,其IC50值低至(4.85±0.61) μmol/L,明显优于阳性药物易瑞沙Iressa(20.17±2.15) μmol/L,LRRC3B mRNA的相对表达量明显升高。

关键词: NCI-H460, 白花丹素衍生物, 细胞增殖, 抑癌基因LRRC3B

Abstract: The anti-tumor effectin vitro of plumbagin derivative (Pln-7-co(oAc)) is investigated.Human lung cancer cell lines (NCI-H460,A549),human liver cancer cell line BEL-7404,human gastric cancer cell line MCG-803 andhuman normal liver cell line HL-7702 were served as the tested cells.The cellproliferation inhibition rates of Pln-7-co(oAc) were detected by MTT colorimetricassay.The relative expression of LRRC3B mRNA after Pln-7-co(oAc) treated wastested by qPCR.The inhibition rate of Pln-7-co(oAc) on NCI-H460 cells (86.68%±2.89%) was much higher than that on A549 cells (59.56%±9.00%),BEL-7404 cells (-7.24%±2.93%),MCG-803 cells (15.27%±5.87%) at a 10 μmol/L concentration.In IC50 test,the inhibition rate of Pln-7-co(oAc) on NCI-H460 cells showed a good dose dependency with IC50 value as low as (4.85±0.61) μmol/L,which was significantly better than the positive drug Iressa (20.17±2.15) μmol/L.The relative expressionofLRRC3B mRNA of Pln-7-co(oAc) group markedly increased compared with that of the control group.

Key words: NCI-H460, plumbagin derivative, cell proliferation, LRRC3B

中图分类号: 

  • R284.3
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