一株产海因酶菌种的筛选与鉴定

广西师范大学学报（自然科学版） ›› 2013, Vol. 31 ›› Issue (1): 119-124.

一株产海因酶菌种的筛选与鉴定

王培^1,2, 曹建华¹, 宋德贵², 辜澜涛², 董研玲³

1.中国地质科学院岩溶地质研究所国土资源部岩溶动力学重点实验室,广西桂林541004;
2.广西师范大学生命科学学院,广西桂林541004;
3.武汉生物工程学院,湖北武汉430415

收稿日期:2012-11-19 出版日期:2013-03-20 发布日期:2018-11-26
通讯作者: 曹建华(1963—),男,江苏海安人,中国地质科学院岩溶地质研究所研究员,博导。E-mail:jhcaogl@karst.ac.cn
基金资助:
中国岩溶碳汇过程与效应(水[2011]矿评01-15-02);国土资源部、广西壮族自治区岩溶动力学重点实验室开放课题、主任基金(KDL2012-04)

Screening and Identification of a Strain of Hydantoinase-producing Microorganism

WANG Pei^1,2, CAO Jian-hua¹, SONG De-gui², GU Lan-tao², DONG Yan-ling³

1.Karst Dynamics Laboratory,Ministry of Land and Resource,Institute of Karst Geology,Chinese Academy of GeologicalSciences,Guilin Guangxi 541004,China;
2.College of Life Science,Guangxi Normal University,Guilin Guangxi 541004,China;
3.Department of Biotechnology,Wuhan Bioengineering Institute,Wuhan Hubei 430415,China

Received:2012-11-19 Online:2013-03-20 Published:2018-11-26

摘要/Abstract

摘要： 通过对土壤中微生物以单一海因类似物作为唯一氮源富集、分离和纯化,利用双层琼脂法筛选获得产海因酶菌株,以基因工程手段提取其DNA,并进行特异性的16S rDNA测序,最终利用Blastn工具将其基因序列与GenBank数据库中的进行序列比对。研究表明:该菌与Bacillus megaterium QM B1551(NC004604)序列相似性达到98%,证明该菌为巨大芽孢杆菌。

关键词: 海因酶, 双层琼脂法, 16S rDNA, 巨大芽孢杆菌

Abstract: Through gathering,isolation and purification,a strain of Hydantoinase-producing microorganisms from soil microbial based on single hydantoin analogue was taken as the onlynitrogen source.The Hydantoinase-producing microorganisms was screened by utilizing the Double agar-agar method.Then,it DNAwas extracted by the means ofgenetic engineering method,and sequence of its specificity 16S rDNA was tested.Lastly,a comparison its gene sequence to GenBank database byBlastn tool.Research result shows:this microorganism has 98% similarity with Bacillus megaterium QM B1551 (NC004604),which proved that it is Bacillus megaterium.

Key words: hydantoinase, double agar-agar method, 16S rDNA, Bacillus megaterium

中图分类号:

Q93-331

王培, 曹建华, 宋德贵, 辜澜涛, 董研玲. 一株产海因酶菌种的筛选与鉴定[J]. 广西师范大学学报（自然科学版）, 2013, 31(1): 119-124.

WANG Pei, CAO Jian-hua, SONG De-gui, GU Lan-tao, DONG Yan-ling. Screening and Identification of a Strain of Hydantoinase-producing Microorganism[J]. Journal of Guangxi Normal University(Natural Science Edition), 2013, 31(1): 119-124.

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