盐酸胍诱导牛血红蛋白变性的微量热研究

广西师范大学学报（自然科学版） ›› 2011, Vol. 29 ›› Issue (4): 88-91.

盐酸胍诱导牛血红蛋白变性的微量热研究

李向荣^1,2, 于洁¹, 郝潇潇²

1.新乡医学院基础医学院,河南新乡453003;
2.河南师范大学化学与环境科学学院,河南新乡453007

收稿日期:2011-06-16 发布日期:2018-11-16
通讯作者: 李向荣(1979—),女,河南长垣人,新乡医学院讲师。E-mail:lixiangrong79@sohu.com
基金资助:
国家自然科学基金资助项目(20673034)

Denaturation Study of Bovine Hemoglobin Induced by Guanidine Chloride by Microcalorimetry

LI Xiang-rong^1,2, YU Jie¹, HAO Xiao-xiao²

1.School of Basic Medicine,Xinxiang Medical University,Xinxiang Henan 453003,China;
2.College of Environmental Science,Henan Normal University,Xinxiang Henan453007,China

Received:2011-06-16 Published:2018-11-16

摘要/Abstract

摘要： 应用恒温微量热技术,对盐酸胍与牛血红蛋白在30 °C水溶液中的结合作用及造成牛血红蛋白变性的过程进行研究,并根据简单结合模型,计算了它们之间的结合常数、结合自由能。用变性中点的直线外推方法求出了表观变性焓。实验结果表明,盐酸胍诱导牛血红蛋白变性的相互作用分为3个阶段。牛血红蛋白在盐酸胍溶液中的变性焓在pH5.21时为200 kJ·mol^-1,在pH6.70时为540 kJ·mol^-1,在pH8.03时为120 kJ·mol^-1。牛血红蛋白在pH6.70时,即在等电点时,较稳定。

关键词: 牛血红蛋白, 恒温微量热, 盐酸胍, 变性

Abstract: The interactions between guanidine chloride and bovine hemoglobin in aqueous solution as well as the denaturation process of bovine hemoglobin were studied by using isothermal microcalorimetry methodat 30 °C and three pHs.The simple binding model was employed to obtain the binding constants and the binding free energies.Furthermore,the linear extrapolationat the midpoint of transition was employed to determine the apparent denaturation enthalpy.The results showed that the interactions between guanidine chloride andbovine hemoglobin is divided into three stages.The apparent denaturation enthalpy of bovine hemoglobin by guanidine chloride is 200 kJ·mol^-1 at pH5.21 and 540 kJ·mol^-1 at pH6.70,while it is 120 kJ·mol^-1 at pH8.03.BHbis most stable at its isoelectric pH.

Key words: bovine hemoglobin, isothermal microcalorimetry, guanidine chloride, denaturation

中图分类号:

O642.3

李向荣, 于洁, 郝潇潇. 盐酸胍诱导牛血红蛋白变性的微量热研究[J]. 广西师范大学学报（自然科学版）, 2011, 29(4): 88-91.

LI Xiang-rong, YU Jie, HAO Xiao-xiao. Denaturation Study of Bovine Hemoglobin Induced by Guanidine Chloride by Microcalorimetry[J]. Journal of Guangxi Normal University(Natural Science Edition), 2011, 29(4): 88-91.

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