斑鳜肌球蛋白轻链1基因cDNA的克隆及其分析

广西师范大学学报（自然科学版） ›› 2011, Vol. 29 ›› Issue (2): 99-103.

斑鳜肌球蛋白轻链1基因cDNA的克隆及其分析

赵发兰, 宾石玉, 陈敦学, 农小献, 刘希良

广西师范大学生命科学学院,广西桂林541004

收稿日期:2010-12-19 发布日期:2018-11-19
通讯作者: 宾石玉(1963—),男,广西全州人,广西师范大学教授,博士。E-mail:binsy68@yahoo.com.cn
基金资助:
国家自然科学基金资助项目(30972263);湖南省教育厅重点资助项目(09A008,10A010);湖南省高校科技创新团队资助项目

cDNA Cloning and Sequence Analysis of the Myosin Light Chain 1 Gene in Siniperca scherzeri

ZHAO Fa-lan, BING Shi-yu, CHEN Dun-xue, NONG Xiao-xian, LIU Xi-liang

College of Life Science,Guangxi Normal University,Guilin Guangxi 541004,China

Received:2010-12-19 Published:2018-11-19

摘要/Abstract

摘要： 为提取斑鳜肌肉组织的总RNA,应用PCR法扩增肌球蛋白轻链1基因(MLC1)。结果获得斑鳜MLC1(基因库登录序列号为:GQ282999),cDNA序列的全长为579 bp,编码192个氨基酸,理论相对分子质量为20 778.57,等电点为4.51,该序列具有2个EF-手相结构。氨基酸序列比对结果表明,第2个EF-手相结构中的Ca²⁺结合域非常保守,氨基酸序列同源性为100%。

关键词: 斑鳜, 肌球蛋白轻链1, cDNA克隆

Abstract: The total RNA was isolated from the skeletal white muscle of Siniperca scherzeri.The myosin light chain 1(MCL1) was amplified using PCR technology,and then was cloned.The length of the myosin light chain 1(MCL1) cDNA is 579 bp,and encodes 192 amino acids,which includes two potential EF-hand structures.The putative protein of MLC1 shows predicted molecular weights of20 778.57 with a theoretical pI of 4.51.Compared with the MLC1 of others reported,the homology of the second potential EF-hand structure of the potential Ca²⁺ binding domain was well conserved among the MLC1s of all species.

Key words: Siniperca scherzeri, myosin light chain 1, cDNA clone

中图分类号:

Q781

赵发兰, 宾石玉, 陈敦学, 农小献, 刘希良. 斑鳜肌球蛋白轻链1基因cDNA的克隆及其分析[J]. 广西师范大学学报（自然科学版）, 2011, 29(2): 99-103.

ZHAO Fa-lan, BING Shi-yu, CHEN Dun-xue, NONG Xiao-xian, LIU Xi-liang. cDNA Cloning and Sequence Analysis of the Myosin Light Chain 1 Gene in Siniperca scherzeri[J]. Journal of Guangxi Normal University(Natural Science Edition), 2011, 29(2): 99-103.

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