广西师范大学学报（自然科学版） ›› 2011, Vol. 29 ›› Issue (1): 43-46.

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十大功劳果实提取物抗氧化活性研究

张业1, 邹碧群1,2, 方毅林1,2, 义祥辉2, 潘英明1   

  1. 1.广西师范大学化学化工学院,广西桂林541004;
    2.桂林师范高等专科学校化学与工程技术系,广西桂林541001
  • 收稿日期:2010-09-13 发布日期:2018-11-16
  • 通讯作者: 潘英明(1972—),男,江西赣州人,广西师范大学教授,博士。E-mail: panym@mailbox.gxnu.edu.cn
  • 基金资助:
    国家973计划前期研究专项(2009CB526503);广西自然科学基金资助项目(0832096,0832024);广西教育厅科研资助项目(200807MS075,200807MS076,200911MS281,200911MS282)

Antioxidant Activity of Extract from Mahonia bealeiFruit

ZHANG Ye1, ZOU Bi-qun1,2, FANG Yi-lin1,2, YI Xiang-hui2, PAN Ying-ming1   

  1. 1.College of Chemistry and Chemical Engineering,Guangxi NormalUniversity,Guilin Guangxi 541004,China;
    2.Department of Chemistry and Engineering Technology,Guilin Normal College,Guilin Guangxi 541001,China
  • Received:2010-09-13 Published:2018-11-16

摘要： 在超声波辅助下,以质量分数为80%的乙醇水溶液对十大功劳果实进行提取,浓缩后得到提取物。在对提取物的DPPH·、ABTS+·清除能力进行研究的基础上,探讨溶剂效应对提取物DPPH·清除活性的影响,并利用荧光猝灭法研究提取物与Cu2+、Fe2+的螯合作用。结果表明,十大功劳果实提取物清除ABTS+·和DPPH·的IC50值均远远小于10 g/L,说明提取物具有良好的自由基清除活性;溶剂的极性对提取物DPPH·清除活性具有重要影响;提取物与Cu2+、Fe2+具有较好的螯合作用,猝灭常数Ksv分别为7.57×102和3.09×103 L·mol-1。

Abstract: Mahonia bealei fruit was extracted using ultrasonic wave to get extract.Based on the study of scavenging activities of extract against DPPH· and ABTS+·,the influence of solvent effect on scavenging activities was studied and the fluorescence-quenching spectroscopy was employed to study their chelation abilities with copper(Ⅱ) and ferrous ion.The results showedthat IC50 of the extract of Mahonia bealei fruit scavenging against DPPH· and ABTS+· were much lower than 10 g/L,implying that the extract had goodscavenging activities,while the solvent effects had important influence on scavenging activities.In addition,the extract of Mahonia bealei fruit showedgood chelation abilities with copper(Ⅱ) and ferrous ion,with the quenching constants Ksv 7.57×102 and 3.09×103 L·mol-1,respectively.

Key words: Mahonia bealei fruit, DPPH·, ABTS+·, chelation, antioxidation

中图分类号: 

  • R282
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