激活蛋白PeFOC1对胁迫条件下红花种子萌发及生长的影响

doi:10.16088/j.issn.1001-6600.2025092801

摘要/Abstract

摘要： 为探究尖孢镰刀菌来源的激活蛋白PeFOC1对盐胁迫与干旱胁迫下红花种子萌发及幼苗生长的调控作用,本文分别采用250 mmol/L NaCl和10% PEG-6000模拟盐胁迫与干旱胁迫条件。实验设置0.1 mg/L (P1)、1 mg/L (P2)、10 mg/L (P3) PeFOC1处理组及对照组(CK),分析其对种子发芽势、发芽指数及幼苗生长指标(株高、根长、茎粗、鲜质量、干质量)的影响,并利用qRT-PCR检测关键免疫基因CtNPR、CtEDS1的表达水平。结果表明,与对照组相比,经1 mg/L PeFOC1 (P2)处理后,红花种子在盐胁迫和干旱胁迫下的发芽势分别显著提高37.15%和61.54%;同时,P2处理显著促进幼苗根系生长,盐胁迫下根干质量增幅超148%。免疫基因表达分析显示,盐胁迫下10 mg/L PeFOC1对CtNPR和CtEDS1的上调作用最显著(相对表达量分别达CK的3.43和7.7倍);而干旱胁迫下,1 mg/L PeFOC1可特异性诱导CtNPR和CtEDS1高表达(相对表达量分别达CK的8.7和5.37倍)。本文研究表明,PeFOC1通过胁迫类型依赖的浓度调控策略,协同调控红花种子萌发、幼苗生理适应性及免疫响应,为红花抗逆栽培及生物抗逆剂的研发提供新思路与理论依据。

关键词: 红花, 胁迫, 种子萌发, 免疫响应, PeFOC1

Abstract: To investigate the regulatory effects of the activator protein PeFOC1 derived from Fusarium oxysporum on safflower seed germination and seedling growth under salt and drought stress, 250 mmol/L NaCl and 10% PEG-6000 were used to simulate salt stress and drought stress conditions, respectively. Treatments included0.1 mg/L (P1), 1 mg/L (P2), and 10 mg/L (P3) of PeFOC1, along with a control group (CK). Parameters such as germination potential, germination index, and seedling growth traits (plant height, root length, stem diameter, fresh weight, and dry weight) were analyzed. The expression of key immune-related genes (CtNPR, CtEDS1) were detected via qRT-PCR. The results demonstrated that comparedwith the untreated stress control group, treatment with 1 mg/LPeFOC1 (P2) significantly enhanced the seed germination potential under both salt and drought stress conditions, with increases of 37.15% and 61.54%, respectively. Meanwhile, P2 treatment markedly promoted root growth, with the root dry weight increasing bymore than 148% under salt stress. Analysis of immune gene expression revealed that under salt stress, 10 mg/L PeFOC1 most significantly up-regulated CtNPR and CtEDS1 (reaching 3.43-fold and 7.7-fold of CK, respectively). In contrast, under drought stress, 1 mg/LPeFOC1 specifically induced high expression of CtNPR and CtEDS1 (reaching 8.7-fold and 5.37-fold of CK, respectively). These findings demonstrate that PeFOC1 coordinately regulates safflower seed germination, seedling physiological adaptation, and immune response through a stress type-dependent concentration strategy, providing new insights and a theoretical basis for stress-resistant cultivation of safflower and the development of biological anti-stress agents.

Key words: Safflower (Carthamus tinctorius L.), abiotic stress, seed germination, immune response, PeFOC1

中图分类号: S567.219;Q945.78

杨涵, 董乐, 周丹宁, 田静蕊, 张文龙, 朱畇昊. 激活蛋白PeFOC1对胁迫条件下红花种子萌发及生长的影响[J]. 广西师范大学学报（自然科学版）, 2026, 44(5): 224-234.

Yang Han, Dong Le, Zhou Danning, Tian Jingrui, Zhang Wenlong, Zhu Yunhao. Modulation of safflower seed germination and seedlinggrowth by activator protein PeFOC1 under stress conditions[J]. Journal of Guangxi Normal University(Natural Science Edition), 2026, 44(5): 224-234.

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