罗汉果叶黄酮对力竭大鼠组织细胞膜保护作用研究

Abstract

Abstract: To provide a theoretic evidence for exploitation andapplication of Siraitia grosvenorii leaves resources,the protection effect of flavonoids extracted from Siraitia grosvenorii leaves in response to oxidative stress injury was studied.Forty healthymale Sprague-Dawley rats (body weigh of 180～220 g,aged 2～3 months) were randomized into five groups,each consisting of 8 rats:the quiet control group,thepro-quiet exhaustive group,the pro-administration exhaustive group,the pro-training exhaustive group and the pro-administration-training exhaustive group.Drug-treated groups were administrated (i.g.) the solution of physiological salinepaired with flavonoids extracted from Siraitia grosvenorii leaves (20 g/L) at a dose of 10.0 mL/kg body weight per day;while other groups were administrated of pure physiological saline at the same dose.After the incremental swimming training of 28 d,rats in exhaustion groups were bore their own weight by 3% and swam to exhaustion.Rats (including the rats in quiet control group) were killed by decapitation immediately after accurately recorded the exhaustive time.Blood,heart,liverand quadriceps tissues were sampled for subsequent analysis of enzymes' activities (GOT,GPT and CK),and observation of histopathological changes with microscope.The results showed that flavonoids extracted from Siraitia grosvenorii leaves can obviously increase the activities of GOT,GPT and CK in heart,liver and quadricepstissues,decrease these corresponding enzymes' activities in plasma.The cell membranes of heart,liver and quadriceps tissues in drug-treated groups are much more integrated than that of non drug-treated groups.The ratios of rupture and deformation are greatly reduced in drug-treated groups.The conclusion indicatesthat flavonoids extracted from Siraitia grosvenorii leaves can help to protect the cell membranes of heart,liver and quadriceps tissues,ensure the integrity and fluidityof the membranes.

Key words: Siraitia grosvenorii leaves, flavonoids, exhaustive exercise, anti-oxidative stress injury

CLC Number:

G804.2

JIANG Feng, DENG Qi-lie, CHEN Mei, MO Wei-bin, YANG Yong-liang. Protection of Flavonoids Extracted from Siraitia grosvenorii Leaves to Different Tissue Membranes in Rats During Exhaustive Exercise[J].Journal of Guangxi Normal University(Natural Science Edition), 2013, 31(2): 140-147.

References

[1] 乔德才,张蕴琨,邓树勋.运动人体科学研究进展与应用[M].北京:人民体育出版社,2008:225-235.
[2] 陈全斌,杨瑞云,义祥辉,等.RP-HPLC法测定罗汉果鲜果及甜甙中总黄酮含量[J].食品科学,2003,24(5):133-135.
[3] 李俊,黄锡山,陈海燕,等.罗汉果多糖提取工艺及组成分析[J].广西师范大学学报:自然科学版,2007,25(1):70-73.
[4] 陈全斌,沈钟苏,韦正波,等.罗汉果黄酮的活血化瘀药理作用研究[J].广西科学,2005,12(4):316-319.
[5] 王勤,王冲,戴盛明,等.罗汉果甜苷对小鼠细胞免疫功能的调节作用[J].中药材,2001,24(11):811-812.
[6] 陈全斌,苏小建,沈钟苏.罗汉果叶黄酮抗氧化能力研究[J].食品研究与开发,2006,27(10):189-191.
[7] 梁英,朱志仁,潘英明,等.罗汉果叶总黄酮的提取及清除自由基活性研究[J].食品科技,2010,35(11):211-213.
[8] 郑楚,唐金良,杨冬业,等.罗汉果总黄酮对实验性糖尿病大鼠的治疗作用[J].中国实验方剂学杂志,2011,17(22):194-197.
[9] 郭艳菊,彭峰林.黄酮对力竭运动大鼠心肌抗氧化与细胞凋亡的影响[J].广西师范大学学报:自然科学版,2012,30(2):143-147.
[10] THOMAS D P,MARSHALL K I.Effects of repeated exhaustive exerciseon myocardial subcellular membrance structures[J].Int J Sports Med,1988(9):257.
[11] 张霞,程富胜.GPA对小鼠肝损伤血清及肝组织中GOT、GPT的影响[J].中国兽医医药杂志,2011(4):17.
[12] 戚世媛,熊正英.女贞子提取物对大鼠心肌的保护作用及对运动能力的影响[J].山东体育学院学报,2011,27(1):53-57.
[13] 潘燕,彭彦铭.补充蜂胶黄酮对过度训练小鼠心肌能量代谢酶的影响[J].武汉体育学院学报,2010,44(8):36-38.
[14] 方允中,陈能乾.医学酶学[M].北京:人民卫生出版社,1984:238.
[15] 吕圭源,陈素红,张丽丹,等.铁皮石斛对小鼠慢性酒精性肝损伤模型血清2种转氨酶及胆固醇的影响[J].中国实验方剂学杂志,2010,16(6):192.
[16] 毕秋芸,彭莉,王启荣.递增负荷训练后大鼠血清CK及其同工酶、LDH、ALT、AST活性变化[J].中国运动医学杂志,2006,25(5):593-595.
[17] 刘宁,王轲,马兰军,等.沙苑子总黄酮对运动训练大鼠血液中某些生化指标影响的实验研究[J].青海医学院学报,2006,27(2):81-85.
[18] 张蕴琨,冯伟权.运动性骨骼损伤时某些生化变化[J].体育科学,1994,14(3):72.
[19] 张蕴琨,丁锡琴,蒋晓玲.举重训练对血清酶和肌红蛋白水平的影响[J].体育科学,1991,11(4):55.
[20] 崔玉鹏.补充复方1,6-二磷酸果糖及谷氨酰胺对训练大鼠游泳运动能力和运动后骨骼损伤的影响[J].中国运动医学杂志,2006,25(1):26.
[21] 李永峰,韩勇,郭勇力,等.黄芪注射液对2种力竭运动大鼠血清肌酸激酶及其同工酶活性的影响[J].中国实验方剂学杂志,2010,16(8):224.
[22] 吴鹤群,王勇.黄柳菇总黄酮对过度运动大鼠骨骼肌组织中自由基代谢水平的影响与机制探讨[J].绵阳师范学院学报,2012,31(2):133-136.
[23] 上原万里子.类黄酮的抗氧化作用[J].日本医学介绍,2005,26(4):155.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Protection of Flavonoids Extracted from Siraitia grosvenorii Leaves to Different Tissue Membranes in Rats During Exhaustive Exercise

Abstract

Cite this article

share this article

References

Related Articles 5

Metrics

Comments

Recommended 0

[1]	LIANG Yan. Non-alkaloid Chemical Constituents from the Chinese Medical Plant Stephania dielsiana [J]. Journal of Guangxi Normal University(Natural Science Edition), 2018, 36(1): 95-98.
[2]	YE Ju, SUN Liqing, JI Shouxiang. Response Surface Optimization of Flavonoids Extraction from Nepeta coerulescens [J]. Journal of Guangxi Normal University(Natural Science Edition), 2017, 35(1): 62-68.
[3]	WANG Juan, QIN Jingfang,LIU Chunyan,TANG Huang. α-Glucosidase and Acetylcholinesterase Inhibitory Potency of Extract from Rhizome of Ficus pumila L. [J]. Journal of Guangxi Normal University(Natural Science Edition), 2017, 35(1): 69-74.
[4]	HUANG Dong, LI Yuelin, BU Kai, HUANG Wen, MO Weibin. Effects of Glycyrrhiza Flavonoids on Lipid Metabolism Enzymeand Expression of ERRα mRNA and Protein of Exhaustive Rats [J]. Journal of Guangxi Normal University(Natural Science Edition), 2016, 34(1): 168-173.
[5]	WEI Bo, YANG Rui-yun, LIU Jing, XUE Jia-jin, SU Xiao-ying, YAO Qian-yun. Extraction Techniques of Total Flavonoids in Flemingia Strobilifera [J]. Journal of Guangxi Normal University(Natural Science Edition), 2012, 30(2): 83-87.