甘蔗糖蜜酒精废液产腐殖酸的菌株筛选与鉴定

Journal of Guangxi Normal University(Natural Science Edition) ›› 2013, Vol. 31 ›› Issue (2): 87-92.

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Isolation and Identification of Microorganism Producing Humic Acid with Sugarcane Molasses Alcohol Wastewater

LI Nan^1,2,3, DENG Zhi-nian^1,3, FENG Ling-bo², ZHOU Rui-fang², WEI Yuan-wen^1,3, CAO Hui-qing^1,3, LI Yang-rui^1,3

1.Guangxi Academy of Agricultural Sciences,Nanning Guangxi 530007,China;
2.College of Life Science and Technology,Guangxi University,Nanning Guangxi 530004,China;
3.Guangxi CropGenetic Improvement and Biotechnology Key Laboratory,Nanning Guangxi 530007,China

Received:2013-03-16 Online:2013-06-20 Published:2018-11-26

Abstract

Abstract: Microbial strain AS019 suited to grow in vinasse andbe able to produce rich HA,was screened from soil.Combined with the colony morphology,physiological and biochemical characteristics and 16S rDNA ITS sequence,AS109 was preliminarily identified as Bacillus megaterium.After fermented by strain AS019,the content of humic acid in vinasse was increased from 6.60 g/L to25.33 g/L.Humic acid structure determined by infrared spectra is rich in activefunctional groups,such as hydroxyl (OH),carboxyl (-COOH),carbonyl (-C=O),amino and high aromaticity as well.The results indicated that vinasse could be used as microbiological medium toproduce the high value-added humic acid.It was of great significance to the utilization of vinasse,environmental protection and circular economy development inthe sugarcane industry.

Key words: alcohol wastewater, humic acid, isolation and identification of microorganism, physiological and biochemical characters, infrared spectral

CLC Number:

Q93-33

LI Nan, DENG Zhi-nian, FENG Ling-bo, ZHOU Rui-fang, WEI Yuan-wen, CAO Hui-qing, LI Yang-rui. Isolation and Identification of Microorganism Producing Humic Acid with Sugarcane Molasses Alcohol Wastewater[J].Journal of Guangxi Normal University(Natural Science Edition), 2013, 31(2): 87-92.

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