Journal of Guangxi Normal University(Natural Science Edition) ›› 2022, Vol. 40 ›› Issue (2): 200-207.doi: 10.16088/j.issn.1001-6600.2021011301

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Changes of Sdr9c7 Gene Expression in Acute Lung Injury Model

HE Sinuo1,2, LI Yinling1,2, ZHOU Jing1,2, ZHOU Jie1,2, LIN Wanhua1,2*, YANG Wenxian3*   

  1. 1. Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology (Guangxi Normal University), Guilin Guangxi 541004, China;
    2. College of Life Sciences, Guangxi Normal University, Guilin Guangxi 541006, China;
    3. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2021-01-13 Revised:2021-03-06 Published:2022-05-31

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Abstract: To observe the expression of Sdr9c7 gene in the lung tissue of mice with acute lung injury (ALI) and explore its mechanism. 9 female C57BL/6 mice were randomly divided into blank group, 3rd-day group and 7th-day group, with 3 mice in each group. Acute lung injury model was induced by lipopolysaccharide (LPS). The experiment was terminated on the 3rd day or on the 7th day of LPS induction respectively. PBS solution was intranasal instilled in the blank group as control group. A549 cells were transfected with SDR9C7-siRNA and the effect of knockdown of Sdr9c7 gene expression was verified. A549 cells were divided into control group, induction group, knockdown group and combination group. Hematoxylin eosin (HE) staining was used to observe the morphology of lung tissue and evaluate the pathological score. The relative expression levels of IL-1β, TNF-α, IL-6 and Sdr9c7 genes in lung tissue and cells were detected by real-time quantitative PCR (RT-qPCR). Results showed that compared with the blank group, the pulmonary edema score, inflammation score, total pathological score and the relative expression of Sdr9c7, IL-1β, TNF-α and IL-6 genes in the 3rd and 7th day groups were increased (all P<0.05); compared with the 3rd day group, the inflammation score, total pathological score and the relative expression of Sdr9c7, IL-1β and TNF-α genes in the 7th day group were decreased (all P<0.05). The relative expression of Sdr9c7 gene was positively correlated with pathological score (r=0.964, P<0.01). In the experiment of knockdown of Sdr9c7 gene, the effect of SDR9C7-siRNA3 was more significant. In the experiment of SDR9C7-siRNA3 knockdown in LPS induced A549 cells, the relative expression levels of IL-1β, TNF-α, IL-6 and Sdr9c7 were detected by RT-qPCR. Compared with the control group, the expression levels of IL-1β, TNF-α, IL-6 and Sdr9c7 increased in the induction group (all P<0.05), decreased in the knockdown group and combination group (all P<0.05), and the expression levels of IL-1β, TNF-α, IL-6 and Sdr9c7 in the knockdown group were lower than those in the combination group (all P<0.05). It suggests that the abnormal expression of Sdr9c7 gene in lung tissue of acute lung injury model mice may be related to LPS induced inflammatory response.

Key words: acute lung injury model, lipopolysaccharide, inflammatory response, knockdown, Sdr9c7 gene

CLC Number: 

  • R563
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