贝莱斯芽孢杆菌 YWC-01全基因组测序及生物学特性分析

doi:10.16088/j.issn.1001-6600.2025061301

Abstract

Abstract: This study characterizes Bacillus velezensis YWC-01, a strain isolated from Daqu with biodegradative activity against deoxynivalenol (DON), and evaluates its probiotic potential through whole-genome sequencing and biological trait analysis. Primary and secondary screening via dilution-plate streaking isolated the strain from Daqu, followed by identification. Whole-genome sequencing employed Illumina NovaSeq and PacBio Sequel platforms. DON degradation efficiency was quantified using high-performance liquid chromatography (HPLC). Genomic functional annotation utilized multiple databases. Metabolic traits were assessed by predicting carbohydrate-active enzyme (CAZy) families and secondary metabolite gene clusters, alongside virulence factor screening and antibiotic resistance mechanism evaluation. Phylogenetic analysis based on gyrA gene elucidated evolutionary relationships. The results showed that, YWC-01 degraded DON at a maximum rate of 29.1% (initial concentration: 140 mg/L). Its genome spanned 4.04 Mb with 46.51% G+C content, encoding 3 928 genes (including 87 tRNAs and 131 CAZy families). AntiSMASH predicted 13 secondary metabolite gene clusters, including those for macrolactin H, bacillaene, and fengycin synthesis. YWC-01 exhibits limited pathogenicity and harbors genetic potential for diverse functional metabolites, supporting its utility as a probiotic agent.

Key words: Bacillus velezensis, deoxynivalenol, whole genome sequencing, biological characteristics, safety

CLC Number: Q933; TS201.3

LEI Ying, XU Bowen, QIN Haixiong, HUANG Xinyi, ZHAO Jiayuan, DU Juan. Whole Genome Sequencing and Biological Characterization of Bacillus velezensis YWC-01[J].Journal of Guangxi Normal University(Natural Science Edition), 2026, 44(3): 148-162.

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Whole Genome Sequencing and Biological Characterization of Bacillus velezensis YWC-01

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