Journal of Guangxi Normal University(Natural Science Edition) ›› 2019, Vol. 37 ›› Issue (3): 132-141.doi: 10.16088/j.issn.1001-6600.2019.03.015

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Spectral Features, α-glucosidase Inhibitory Activities, Antioxidant Activities and Quantitative Determination of Mogroside ⅡA of Siraitia grosvenorii

CHEN Xu1, LIANG Chengqin1*   

  1. 1.College of Pharmacy, Guilin Medical University, Guilin Guangxi 541004,China;
    2.College of Biotechnology, Guilin Medical University, Guilin Guangxi 541004,China
  • Online:2019-07-12 Published:2019-07-12

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Abstract: In order to study the biological activity and resource content of the sweet glycosides in the fruits of Siraitia grosvenorii and provide experimental basis for its further development and utilization, the compound of mogroside ⅡA was isolated from the fruits by column chromatography. NMR was used to analyze the spectral characteristics of mogroside ⅡA; The possible mechanism of hypoglycemia of mogroside was discussed through the determination of the inhibitory activity of α-glucosidase and antioxidant activities; And by HPLC-MS method, the content of mogroside ⅡA of Siraitia grosvenorii from different habitats were detected. As a result, this paper provides complete spectrum data for mogroside ⅡA that has not been reported; And the results showed that mogroside ⅡA had good inhibiting activity of α-glucosidase, the IC50was 0.187±0.006 g/L, and the inhibiting activity showed a dose-dependent, fast, competitive and reversible features; In addition, through content analysis, it was found that the content of mogroside ⅡA of Siraitia grosvenorii produced from Yongfu township was the highest.

Key words: Siraitia grosvenorii, mogroside ⅡA, HPLC-MS, α-glucosidase activity, antioxidant activity

CLC Number: 

  • R284.1
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