Journal of Guangxi Normal University(Natural Science Edition) ›› 2019, Vol. 37 ›› Issue (1): 211-217.doi: 10.16088/j.issn.1001-6600.2019.01.025

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Correlation Analysis of Polymorphism and Its Cold Tolerance Traits HSP70 Gene of GIFT Tilapia (Oreochromis niloticus)

BIN Shiyu1, ZHONG Dandan1, DU Xuesong2,ZHANG Yongde2, LIN Yong2*, HUANG Yin2,WEN Luting2   

  1. 1.College of Life Science, Guangxi Normal University/Key Laboratory of Ecology of Rare and Endangered Speicies and Environmental Protection, Ministsry of Education, Guilin Guangxi 541006,China;
    2.Guangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning Guangxi 530021,China
  • Received:2018-06-16 Online:2019-01-20 Published:2019-01-08

Abstract: The polymorphisms of HSP70 in the GIFT tilapia samples were detected by using PCR-SSCP technique and the correlation with cold tolerance capability of GIFT tilapia was analyzed by least square method in this study. The results showed that the electrophoresis diagram of the primer HP70-1, HP70-4, HP70-6 designed according to the HSP70 has the polymorphism of the belt type in different cold tolerance samples. The polymorphism of the gene was significantly correlated with its cold tolerance. Therefore, it is inferred that HSP70 genes are potential cold-resistant genes of tilapia.

Key words: GIFT tilapia (Oreochromis niloticus), cold tolerance traits, polymorphism, PCR-SSCR, HSP70 gene

CLC Number: 

  • Q953.3
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[1] XU Yilan, ZHONG Dandan, DU Xuesong, XIE Yandong, LIU Jingjie, SUN Yue, BIN Shiyu. Correlation Analysis of Polymorphism and Its Cold Tolerance Traits TCP-1-eta Gene of GIFT Tilapia (Oreochromis niloticus) [J]. Journal of Guangxi Normal University(Natural Science Edition), 2020, 38(5): 112-117.
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