Journal of Guangxi Normal University(Natural Science Edition) ›› 2018, Vol. 36 ›› Issue (4): 124-130.doi: 10.16088/j.issn.1001-6600.2018.04.016

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Protective Effect of Magnesium-L-Threonateon Dopamine Neurons and Its Mechanism

LUO Raoshan1, SHEN Yanling2,3, SUN Liyuan1*   

  1. 1.Guangxi Key Laboratory of Brain and Cognitive Neuroscience,Guilin Guangxi 541004,China;
    2.Department of Pathology, Affiliated Hospital of Guilin Medical University, Guilin Guangxi 541004, China;
    3.Successful Affiliated Hospital of Xiamen University,Xiamen Fujian 361000, China
  • Received:2017-12-04 Published:2018-10-20

Abstract: To evaluate the neuroprotective effect of Magnesium-L-threonate (MgT) on mice model of Parkinson’s disease (PD) induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) and to investigate the possible mechanism.The PD mice model was established. The mice were randomly divided into control group, MPTP group and the MPTP+MgT group. Rotary bar method was used to observe behavioral changes in mice. The number of dopaminergic neurons in substantia nigra was observed by TH staining. The expression of GFAP, iNOS and TH in substantia nigra and striatum were detected by Western blotting.The time for the MPTP+MgT group to stay on the rotating rod was 68.07% longer than that in the MPTP group. The results showed that, in MPTP+MgT group, the number of dopamine neurons in the substantia nigra increased by 46.15% than that in the MPTP group. Compared with MPTP group, the expression of GFAP and iNOS in MPTP+MgT group was statistically significant (P<0.05).The mechanism of MgT neuroprotective effect is probably related to the down-regulation of GFAP and iNOS expression,and inhibition of inflammation and oxidative stress.

Key words: Parkinson’s disease, Magnesium-L-threonate, tyrosine hydroxylase, glial fibrillary acidic protein, inducible nitric oxide synthase

CLC Number: 

  • R742.5
[1] ZHENG Meizhu,LIU Chunming,FAN Yajun,et al.Neuroprotection by Paeoniflorin in the MPTP mouse model of Parkinson’s disease[J].Neuropharmacology,2017,116:412-420.
[2] KAUR B,PRAKASH A.Ceftriaxone attenuates glutamate-mediated neuro-inflammation and restores BDNF in MPTP model of Parkinson’s disease in rats[J].Pathophysiology,2017,24(2):71-79.
[3] PRZEDBORSKI S,VILA M.The 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine mouse model: a tool to explore the pathogenesis of Parkinson’s disease[J].Annals New York Academy of Sciences,2003,991(1):189-198.
[4] SLUTSKY I,ABUMARIA N,WU Longjun,et al.Enhancement of learning and memory by elevating brain magnesium[J].Neuron,2010,65(2):165-177.
[5] SHINDO Y,YAMANAKA R,SUZUKI K,et al.Altered expression of Mg(2+) transport proteins during Parkinson’s disease-like dopaminergic cell degeneration in PC12 cells[J].Biochimica et Biophysica Acta,2016,1863(8):1979-1984.
[6] PLATO C C,GARRUTO R M,GALASKO D,et al.Amyotrophic lateral sclerosis and parkinsonism-dementia complex of Guam: changing incidence rates during the past 60 years[J].American Journal of Epidemiology ,2003,157(2):149-157.
[7] BARBIROLI B,MARTINELLI P,PATUELLI A,et al.Phosphorus magnetic resonance spectroscopy in multiple system atrophy and Parkinson’s disease[J].Movement Disorders,1999,14(3):430-435.
[8] WANG Pu,YU Xin,GUAN Peipei,et al.Magnesium ion influx reduces neuroinflammation in Aβ precursor protein/Presenilin 1 transgenic mice by suppressing the expression of interleukin-1β[J]. Cellular and Molecular Immunology,2017,14(5):451-464.
[9] HASHIMOTO T,NISHI K,NAGASAO J,et al.Magnesium exertsboth preventive and ameliorating effects in an in vitro rat Parkinson disease model involving 1-methyl-4-phenylpyridinium (MPP+) toxicity in dopaminergic neurons[J].Brain Resarch,2008,1197:143-151.
[10] ROZAS G1, LÓPEZ-MARTÍN E, GUERRA M J,et al.The overall rod performance test in the MPTP-treated-mouse model of Parkinsonism[J]. Journal of Neuroscience Methods, 1998, 83(2): 165-175.
[11] 于龙川.神经生物学[M].北京:北京大学出版社,2012:113.
[12] MARTÍY, MATTHAEUS F, LAU T,et al. Methyl-4-phenylpyridinium (MPP+) differentially affects monoamine release and re-uptake in murine embryonic stem cell-derived dopaminergic and serotonergic neurons[J]. Molecular and Cellular Neuroscience, 2017, 83:37-45.
[13] KOKOVAY E,CUNNINGHAM L A.Bone marrow-derived microglia contribute to the neuroinflammatory response and express iNOS in the MPTP mouse model of Parkinson’s disease[J].Neurobiology of Disease,2005,19(3): 471-478.
[14] GOSWAMI P,JOSHI N,SINGH S.Neurodegenerative signaling factors and mechanisms in Parkinson’s pathology[J].Toxicology in Vitro,2017,43:104-112.
[15] SUN Yuyang,SUKUMARAN P,SCHAAR A,et al.TRPM7 and its role in neurodegenerative diseases[J].Channels (Austin),2015,9(5):253-261.
[16] HSIEH C C,LIN M S,HUA Kuofeng,et al.Neuroprotection by freshwater clam extract against the neurotoxin MPTP in C57BL/6 mice[J].Neuroscience Letters, 2017,642:51-58.
[17] YAMADA M,KIDA K,AMUTUHAIRE W,et al.Gene disruption of caspase-3 prevents MPTP-induced Parkinson’s disease in mice[J].Biochemical and Biophysical Research Communications,2010,402(2):312-318.
[18] JIMÉNEZ-JIMÉNEZ F J,ALONSO-NAVARRO H,HERRERO M T,et al.An update on the role of nitric oxide in the neurodegenerative processes of Parkinson’s disease[J].Current Medicinal Chemistry, 2016, 23(24):2666-2679.
[19] 任博,孙法威,张作凤,等.丹参酮ⅡA对帕金森病模型小鼠多巴胺能神经元的保护作用及其机制[J].吉林大学学报(医学版),2014,40(5):947-952.
[20] 王茜,张辉,刘名,等.P38信号通路调控帕金森病小鼠黑质NF-κB和iNOS的表达[J].南方医科大学学报,2014, 34(8):1176-1180.
[21] WU Duchu,JACKSON-LEWIS V,VILA M,et al.Blockade of microglial activation is neuroprotective in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine mouse model of Parkinson’s disease[J]. Journal of Neuroscience,2002,22(5):1763-1771.
[22] BROOM L,MARINOVA-MUTAFCHIEVA L,SADEGHIAN M,et al.Neuroprotection by the selective iNOS inhibitor GW274150 in a model of Parkinson disease[J].Free Radical Biology and Medicine,2011,50(5):633-640.
[23] WU Anguo,ZENG Wu,WONG V K-W,et al.Hederagenin and α-hederin promote degradation of proteins inneurodegenerative diseases and improve motor deficits in MPTP-mice[J].Pharmacological Research,2017,115:25-44.
[24] EBRAHIMI S S,ORYAN S,IZADPANAH E,et al.Thymoquinone exerts neuroprotective effect in animal model of Parkinson’s disease[J].Toxicology Letters,2017,276:108-114.
[25] LI Huan,PARK G,BAE N,et al.Anti-apoptotic effect of modified Chunsimyeolda-tang,a traditional Korean herbal formula,on MPTP-induced neuronal cell death in a Parkinson’s disease mouse model[J]. Journal of Ethnopharmacology,2015,176:336-344.
[26] YADAV S K,PRAKASH J,CHOUHAN S,et al.Comparison of the neuroprotective potential of Mucuna pruriens seed extract with estrogen in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced PD mice model[J].Neurochemistry International,2014,65:1-13.
[27] SIRACUSA R,PATERNITI I,CORDARO M,et al.Neuroprotective effects of temsirolimus in animal models of Parkinson’s disease[J].Molecular Neurobiology,2017,55(1):1-17.
[28] CAO Qin,QIN Liyue,HUANG Fei,et al.Amentoflavone protects dopaminergic neurons in MPTP-induced Parkinson’s disease model mice through PI3K/Akt and ERK signaling pathways[J].Toxicology and Applied Pharmacology,2017,319:80-90.
[29] MEENAKSHI S,UMAYAPARVATHI S,SARAVANAN R,et al.Neuroprotective effect of fucoidan from Turbinaria decurrens in MPTP intoxicated Parkinsonic mice[J].International Journal of Biological Macromolecules, 2016,86:425-433.
[30] GUO Baojian,HU Shengquan,ZHENG Chengyou,et al.Substantial protection against MPTP-associated Parkinson’s neurotoxicity in vitro and in vivo by anti-cancer agent SU4312 via activation of MEF2D and inhibition of MAO-B[J].Neuropharmacology,2017,126:12-24.
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