产酸克雷伯氏菌基因组文库的构建及鉴定

doi:10.16088/j.issn.1001-6600.2016.02.023

Journal of Guangxi Normal University(Natural Science Edition) ›› 2016, Vol. 34 ›› Issue (2): 151-157.doi: 10.16088/j.issn.1001-6600.2016.02.023

Construction and Characterization of the GenomicLibrary of Klebsiella oxytoca

JIANG Shaofeng^1,2,3, HUANG Jinqing^1,2, YU Xiaoyu^1,2, LI Yunfei^1,2, LU Zujun^1,2

1.College of Life Science, Guangxi Normal University, Guilin Guangxi 541006,China;
2.Key Laboratory of Ecologyof Rare and Endangered Species and Environmental Protection(Ministry of Education),Guilin Guangxi 541006,China;
3. College of Agriculture,South China Agriculture University,Guangzhou Guangdong 510462,China

Received:2015-07-18 Online:2016-06-25 Published:2018-09-14

Abstract

Abstract: The 1 329-clones genomic library of Klebsiella oxytoca KO108 was constructed by pWEB^TM Cosmid. Its coverage and randomness was confirmed by the electrophoresis profiles of 20 recombination plasmids digestion with EcoR Ⅰ,which were randomly selected from the library. The results showed that all tested plasmids contained the 8.2 kb cosmid fragment and an exogenous one diverse in size from 25 to 50 kb(about 40 kb in average), this suggested that the total capacity of this library was about 52 Mb, 5 times of KO108 genome, 99.8% theoretical possibility including any gene in this strain. The library lays a foundation for functional gene cloning and comparative genomics research for Klebsiella oxytoca.

Key words: Klebsiella oxytoca, genomic library, pWEB^TM Cosmid, coverage, library capacity

CLC Number:

Q933

JIANG Shaofeng , HUANG Jinqing , YU Xiaoyu , LI Yunfei , LU Zujun. Construction and Characterization of the GenomicLibrary of Klebsiella oxytoca[J].Journal of Guangxi Normal University(Natural Science Edition), 2016, 34(2): 151-157.

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