Journal of Guangxi Normal University(Natural Science Edition) ›› 2014, Vol. 32 ›› Issue (4): 126-134.

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Preliminary Studies on Ultrastructure and Genetic Inactivity of Spermatozoa in Ltiopenaeus vannamei

ZHANG Bin, WEI Pin-yuan, XIONG Jian-hua, XIE Da-xiang, ZHAO Yong-zhen, CHEN Xiao-han   

  1. Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Science, Nanning Guangxi 530021, China
  • Received:2014-06-29 Published:2018-09-26

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Abstract: The genetic inactivity of sperm in Litopenaeus vannamei by UV at 365 nm wavelength and 10.5 cm radiation distance were investigated. The inactivity sperm or spermatophore was used to fertilize by artificial insemination methods. The results indicated the fertilization of fresh semendiluter happened during 0~20 s radiation, and which could obtain 5% of average fertility rate by vitro fertilization method, yet all of embryos would not develop to nauplius. The average fertility rate and average hatching rate of fresh spermatophore by semen transplantation were 20% and 3% respectively during 0~10 min radiation. The sperm of fresh semendiluter radiated for more than 30 s would be inactive under the 2×105 sperm/cm2 with 2 mm thickness shaking in 90 time per minute, and the sperm of spermatophore radiated for more than 20 min would be inactive. The morphology and ultrastructure between fresh and different UV dosage radiated spermatozoa of Litopenaeus vannamei were observed by light and transmission electronic microscopy. The observation revealed that the spermatozoa of Litopenaeus vannamei was of the non-motile type. It consisted of three main parts, including the spike-anterior in position, the cap-shaped part at the middle and the main body part at the rear. The results showed that morphology and ultrastructure of sperm from fresh semendiluter UV radiated for 10~20 s didn’t change compared with normal fresh sperm. The acrosome and spike of sperm from fresh semendiluter UV radiated for 30 s were normal, but its chromatin dispersion and bottom wall became thinner. The sperm from fresh semendiluter UV radiated for 60 s was damaged obviously, mainly marked with cell nucleus deformed and membrane cracked, nucleus region diminished, chromatin dispersion and overflow, acrosome body impaired partly, membrane of posterior main body folded, and bottom hollowed, cytoplasmic banding vesiculated, nucleus membrane impaired and cracked, membrane swelled and inter-membrane space augmented.

Key words: Litopenaeus vannamei, spermatozoa, ultrastructure, genetic inactivity

CLC Number: 

  • Q248
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