Journal of Guangxi Normal University(Natural Science Edition) ›› 2014, Vol. 32 ›› Issue (3): 109-115.

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Functional Analysis of a Hypothetical udgH Gene in Xanthomona oryzae pv. oryzicola

CAI Wen-xia1, HUANG Guang-hui1, WANG Ting-ting1, FU Shan1, HE Yong-qiang1,2, JIANG Wei1,2   

  1. 1. College of Life Science and Technology, Guangxi University,Nanning Guangxi 530004,China;
    2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-biosciences, Nanning Guangxi 530004, China
  • Received:2014-03-07 Online:2014-09-25 Published:2018-09-25

Abstract: Xanthomonas oryzae pv. oryzicola (Xoc) is an important plant pathogen, which caused rice bacterial leaf blight and seriously decreased the production of rice. A mutation in putative udgH gene of Xoc leads to serious weakening on extracellular polysaccharide production and virulence, and lower extracellular amylase and cellulase activity. The phenotype of complementary strain can be restored to wild-type levels, while virulence only by 80%, and the extracellular polysaccharide production of high expression strain is increased, while the virulence is decreased. The research laid solid foundation for further study the role of the gene in extracellular polysaccharide metabolic pathways of Xoc, and provided a feasible method for constructing high-yielding strains of xanthan gum useing genetic engineering.

Key words: Xanthomonas oryzae pv. oryzicola, UDP-dehydrogenase gene, Tn5-tagged mutant, extracellular polysaccharide, virulence

CLC Number: 

  • Q93
[1] 黄成栋, 白雪芳, 杜昱光. 黄原胶(Xanthan Gum) 的特性、生产及应用[J]. 微生物学通报, 2005, 32(2):91-98.
[2] JANSSON P, KENNE L, LINDBERG B. Structure of the extracellular polysaccharide from Xanthomonas campestris[J]. Carbohydrate Research, 1975, 45(1):275-282.
[3] HUBLIK G. 10.11-Xanthan[M]. Amsterdam: Elsevier, 2012:221-229.
[4] DENNY T P. Involvement of bacterial polysaccharides in plant pathogenesis[J]. Annual Review of Phytopathology, 1995, 33(1):173-197.
[5] BLANCH M, VICENTE C, PIÑÓN D, et al. Sugarcane glycoproteins are required to the production of an active UDP-glucose dehydrogenase by Xanthomonas albilineans[J]. Annals of Microbiology, 2007, 57(2):217-221.
[6] CLARKIN C E, ALLEN S, WHEELER-JONES C P, et al. Reduced chondrogenic matrix accumulation by 4-methylumbelliferone reveals the potential for selective targeting of UDP-glucose dehydrogenase[J]. Matrix Biology, 2011, 30(3):163-168.
[7] JOHANSSON H, STERKY F, AMINI B, et al. Molecular cloning and characterization of a cDNA encoding poplar UDP-glucose dehydrogenase, a key gene of hemicellulose/pectin formation[J]. Biochimica et Biophysica Acta, 2004, 7(3):277-284.
[8] IELPI L, COUSO R O, DANKERT M A. Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris[J]. Journal of Bacteriology, 1993, 175(9):2490-2500.
[9] TUNG S Y, KUO T T. Requirement for phosphoglucose isomerase of Xanthomanas campestris in pathogenesis of citrus caker[J]. Applied and Environmental Microbiology,1999,65(12):5564-5570.
[10] ZOU H S, YUAN L, GUO W, et al. Construction of a Tn5-tagged mutant library of Xanthomonas oryzae pv. oryzicola as an invaluable resource for functional genomics[J]. Current Microbiology, 2011, 62(3):908-916.
[11] CHANG K W, WENG S F, TSENG Y H. UDP-Glucosedehydrogenase gene of Xanthomonas campestris is required for virulence[J]. Biochemical and Biophysical Research Communications, 2001, 287(2):550-555.
[12] SAMBROOK J. Molecular cloning:a laboratory manual[M]. New York:Cold Spring Harbor Laboratory Press, 2001:1999.
[13] 袁亮, 李玉蓉, 张希福, 等. 热不对称PCR( TAIL-PCR) 和Tn5 转座子质粒拯救法快速分离水稻条斑病菌致病相关基因[J]. 农业生物技术学, 2009, 17(6):1089-1095.
[14] TANG J L, LIU Y N, BARBER C E, et al. Genetic and molecular analysis of a cluster of rpf genes involved in positive regulation of synthesis of extracellular enzymes and polysaccharide in Xanthomonas campestris pv. campestris[J]. Molecular Genetics and Genomics, 1991, 226(3):409-417.
[15] McGINNIS S, MADDEN T L. Blast:at the core of a powerful and diverse set of sequence analysis tools[J]. Nucleic Acids Research, 2004, 32:W20-25.
[16] PONTING C P, SCHULTZ J, MILPETZ F, et al. Smart:identification and annotation of domains from signalling and extracellular protein sequences[J]. Nucleic Acids Research, 1999,27(1):229-232.
[17] THOMPSON J D, HIGGINS D G, GIBSON T J. CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice[J]. Nucleic Acids Research, 1994, 22(22):4673-4680.
[18] TAMURA K, PETERSON D, PETERSON N, et al. MEGA5:molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J]. Molecular Biology and Evolution, 2011, 28(10):2731-2739.
[19] ASLAM S N, NEWMAN M A, ERBS G, et al. Bacterial polysaccharides suppress induced innate immunity by calcium chelation[J]. Current Biology, 2008, 18(14):1078-1083.
[20] 周丹,邹丽芳,邹华松,等. 水稻条斑病菌胞外多糖相关基因的鉴定[J]. 微生物学报, 2011, 51(10):1334-1341.
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