Journal of Guangxi Normal University(Natural Science Edition) ›› 2014, Vol. 32 ›› Issue (2): 137-142.

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A New Gene is Required for Full Virulence of Xanthomonas oryzae pv. oryzicola in Rice

HUANG Sheng1,2, LU Ye1, ZHU Xiao-lin1, PAN Jun-xia1, HE Yong-qiang1,2, JIANG Wei1,2   

  1. 1.College of Life Science and Technology, Guangxi University,Nanning Guangxi 530004,China;
    2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-biosciences, Nanning Guangxi 530004, China
  • Received:2014-01-06 Online:2014-06-25 Published:2018-09-25

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Abstract: Xanthomonas oryzae pv. oryzicola (hereafter Xoc) is one of the main pathogens of rice, which could cause rice bacterial leaf streak, resulting in severe losses in rice production. In this study, a random insertion mutant library of Xoc strain which isolated from Guangxi and named as GX01 was constructed using EZ∷Tn5 transposon. One mutant which showed greatly reduced virulence on rice was identified from the library using puncture virulence assay. The insertion site was mapped by TAIL-PCR in XOC3376, and this gene encodes a hypothetical protein. In order to investigate this genes’s functions, several phenotype and virulence assay of this mutant and complementary strain were checked. The results showed that all phenotype of the complementary strain and virulence have been recovered. The study facilitate elucidating the pathogenesis of this phytopathogen.

Key words: Xanthomonas oryzae pv. oryzicola, EZ∷Tn5 transposon, mutant library, TAIL-PCR, pathogenicity genes

CLC Number: 

  • Q93
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[1] CAI Wen-xia, HUANG Guang-hui, WANG Ting-ting, FU Shan, HE Yong-qiang, JIANG Wei. Functional Analysis of a Hypothetical udgH Gene in Xanthomona oryzae pv. oryzicola [J]. Journal of Guangxi Normal University(Natural Science Edition), 2014, 32(3): 109-115.
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