黄鳝性逆转相关基因F4的克隆和表达分析

Abstract

Abstract: Using rapid-amplification of cDNA ends and semi-quantitative RT-PCR technique,the full length of the differentially expressed gene (F4) was obtained and the expression in the rice field eel (Monopterus albus) was conducted at different gonad developmental stage.The results showed that the full length of F4 was 2 466 base pair (bp) that comprises of a 5'-UTR of 142 bp,a 3'-UTR of 596 bp and an open reading frame(ORF) of 1 728 bp which encoded a 575 amino acid with no signal peptide.Homology analysis suggested that it was a newly-reported gene.F4 did not transcription have any at the early stage of ovary and began to express in ovary at Ⅳ stage,with the ovary abortive and testis development the transcription level was significantly increased.These results may provide molecular base for further study on sex regulation mechanism of the rice field eel.

Key words: Monopterus albus, gonad, differentially expressed gene, RACE, RT-PCR, in situ hybridization

CLC Number:

Q786

JIANG Jiao-yun, FENG Long, QU Xian-cheng, TIAN Wen-fei. Clone and Expression of a Novel Sex-related Gene (F4) in Gonads During Sex Reversal in the Rice Field Eel (Monopterus albus)[J].Journal of Guangxi Normal University(Natural Science Edition), 2013, 31(4): 121-127.

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Clone and Expression of a Novel Sex-related Gene (F4) in Gonads During Sex Reversal in the Rice Field Eel (Monopterus albus)

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