Journal of Guangxi Normal University(Natural Science Edition) ›› 2013, Vol. 31 ›› Issue (1): 67-71.

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In vitro Anti-tumor Effect of Plumbagin Derivative Pln-7-co(oAc)

PENG Yan1,2, CAI Le-jing1, ZHOU Yuan-hua1, LI Zhi1, LIURen-lu1, ZHONG Hui2, ZHANG Guo-hai2   

  1. 1.College of Life Science,Guangxi Normal University,Guilin Guangxi 541004,China;
    2.College of Chemistry and Chemical Engineering,Guangxi Normal University,Guilin Guangxi 541004,China
  • Received:2012-07-06 Online:2013-03-20 Published:2018-11-26

Abstract: The anti-tumor effectin vitro of plumbagin derivative (Pln-7-co(oAc)) is investigated.Human lung cancer cell lines (NCI-H460,A549),human liver cancer cell line BEL-7404,human gastric cancer cell line MCG-803 andhuman normal liver cell line HL-7702 were served as the tested cells.The cellproliferation inhibition rates of Pln-7-co(oAc) were detected by MTT colorimetricassay.The relative expression of LRRC3B mRNA after Pln-7-co(oAc) treated wastested by qPCR.The inhibition rate of Pln-7-co(oAc) on NCI-H460 cells (86.68%±2.89%) was much higher than that on A549 cells (59.56%±9.00%),BEL-7404 cells (-7.24%±2.93%),MCG-803 cells (15.27%±5.87%) at a 10 μmol/L concentration.In IC50 test,the inhibition rate of Pln-7-co(oAc) on NCI-H460 cells showed a good dose dependency with IC50 value as low as (4.85±0.61) μmol/L,which was significantly better than the positive drug Iressa (20.17±2.15) μmol/L.The relative expressionofLRRC3B mRNA of Pln-7-co(oAc) group markedly increased compared with that of the control group.

Key words: NCI-H460, plumbagin derivative, cell proliferation, LRRC3B

CLC Number: 

  • R284.3
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