毛冬青叶三萜酸提取纯化研究

Journal of Guangxi Normal University(Natural Science Edition) ›› 2012, Vol. 30 ›› Issue (2): 88-93.

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Extraction of Total Triterpenoids from Ilex pubescens Hook leaves

YIN Wen-qing, FENG Hua-fen, DUAN Shao-qing, TAN Qiang, CEN Ning-cai

State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Chemical Engineering,Guangxi Normal University,Guilin Guangxi 541004,China

Received:2011-07-21 Online:2012-06-20 Published:2018-12-03

Abstract

Abstract: The extraction process of triterpenoids from Ilex pubescens Hook leaves was investigated.The triterpenoids composition was determined by spectrumetric method with oleanolic acid as a reference.The influence of extracting times,radio of liquid to material,extracting time and concentration of ethanol on the triterpenoids of yield were evaluated by single factor experiments and orthogonal experiments.The results showed that the optimum conditions were 1∶16 in radio of liquid to material,concentration of ethanol70%,extraction time 90 min and extracting two times.The content of triterpenoids was 6.18% in extract.

Key words: Ilex pubescens Hook leaves, triterpenoids, extraction process, orthogonal test

CLC Number:

O629

YIN Wen-qing, FENG Hua-fen, DUAN Shao-qing, TAN Qiang, CEN Ning-cai. Extraction of Total Triterpenoids from Ilex pubescens Hook leaves[J].Journal of Guangxi Normal University(Natural Science Edition), 2012, 30(2): 88-93.

References

[1] 江苏新医学院编.中医药大辞典:上册[S].上海:上海人民出版社,1977:441-443.
[2] 邓六勤,钟鸣,马志铃.毛冬青化学成分、药理作用及应用研究进展[J].中国中药现代远程教育,2004,4(10):24-26.
[3] 乔宛虹.毛冬青的药理作用及临床应用研究概况[J].中国现代药物应用,2008,2(5):104-106.
[4] 许丽清.冠心宁汤治疗冠心病60例疗效观察[J].淮海医药,1997(1):6.
[5] 蔡雄,刘中秋,祝晨,等.毛冬青化学成分、药理作用及临床应用研究发展[J].广东药学,2001,11(1):4-6.
[6] 杨运云,黄深惠,冯锋,等.加速溶剂萃取/HPLC-MS对毛冬青药材中IlexgeninA与Ilexsaponin A1含量的测定[J].分析测试学报,2009,28(8):966-969.
[7] 蒋仲芳,黄柔湘,秦国伟,等.毛冬青化学成分的研究:Ⅲ:四种三萜皂甙的分离和鉴定[J].中草药,1991,22(7):291-294.
[8] 杨运云,郭鹏然,陈怡禄,等.高效液相色谱质谱法快速鉴定复方毛冬青冲剂中三萜皂苷活性成分[J].分析化学研究简报,2009,37(10):1523-1527.
[9] 原素.毛冬青提取纯化工艺研究[D].广州:广州中医药大学中药新药开发中心,2008:19-27.
[10] 周中流,邹节明,王力生,等.毛冬青根中木脂素配糖体提取工艺及纯化鉴定研究[J].时珍国医国药,2008,19(4):868-869.
[11] 尹文清,周中流,邹节明,等.毛冬青总皂苷的提取工艺及纯化鉴定研究[J].中药材,2007,30(9):1153-1155.
[12] 旷春桃,王清.毛冬青根中总黄酮的提取工艺研究[J].安徽农业科学,2009,37(1):180-l81.
[13] 周中流.毛冬青化学成份的研究[D].桂林:广西师范大学化学化工学院,2007:49-50.
[14] 皮文霞,蔡宝昌,郭胜伟,等.分光光度法测定山茱萸制剂中总三萜酸的含量[J].南京中医药大学学报,2003,19(2):99-100.
[15] 丁轲,崔莹,陆晶晶,等.SP700大孔树脂纯化酸枣仁中三萜总皂苷的研究[J].离子交换与吸附,2011,27(1):33-42.

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Extraction of Total Triterpenoids from Ilex pubescens Hook leaves

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