Abstract: Based on specific binding of antigen and antibody, western blot analysis is used to detect a protein in complex samples. However, using conventional chemiluminescence detection, light of the protein band is weak and X film is not much exposed, and using ultra-enhanced chemiluminescence detection,light of the protein band is strong and X film is too much exposed, both development images of protein band are not ideal sometimes, when protein concentration of the samples is too low, especially when the protein to be detected is lower abundant in X film is samples. Therefore, the protein was detected with diluted ultra-enhanced chemiluminescent solution, and the protein band was clearly showed and the development image effect of protein bands was improved obviously, which indicates that the intensity of light emission could be reduced and the development image effect of the protein bands could be improved by dilution of ultra-enhanced chemiluminescent solution in western blot analysis.

Key words: western blot, ultra-enhanced chemiluminescent solution, chemiluminescence, dilution