广西师范大学学报（自然科学版） ›› 2014, Vol. 32 ›› Issue (3): 109-115.

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水稻细菌性条斑病菌一个假定的udgH基因的功能研究

蔡文侠1, 黄光辉1, 王婷婷1, 付珊1, 何勇强1,2, 姜伟1,2   

  1. 1.广西大学 生命科学与技术学院,广西 南宁 530004;
    2.亚热带农业生物资源保护与利用国家重点实验室,广西 南宁 530004
  • 收稿日期:2014-03-07 出版日期:2014-09-25 发布日期:2018-09-25
  • 通讯作者: 姜伟(1975—),男(瑶族),广西桂林人,广西大学副教授。E-mail:weijiang@gxu.edu.cn
  • 基金资助:
    国家自然科学基金资助项目(31260261、31360021);广西自然科学基金资助项目(20130231);广西教育厅基金资助项目(20130300)

Functional Analysis of a Hypothetical udgH Gene in Xanthomona oryzae pv. oryzicola

CAI Wen-xia1, HUANG Guang-hui1, WANG Ting-ting1, FU Shan1, HE Yong-qiang1,2, JIANG Wei1,2   

  1. 1. College of Life Science and Technology, Guangxi University,Nanning Guangxi 530004,China;
    2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-biosciences, Nanning Guangxi 530004, China
  • Received:2014-03-07 Online:2014-09-25 Published:2018-09-25

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摘要： 水稻细菌性条斑病菌(Xanthomonas oryzae pv.oryzicola, Xoc)是一种重要植物病原菌,其引发的水稻细菌性条斑病导致水稻严重减产。Xoc一个假定的udgH基因的突变,导致Xoc产胞外多糖和致病力的严重减弱,并使胞外淀粉酶和纤维素酶的酶活也有一定的降低,互补菌株能恢复到野生型水平,但致病力仅能恢复到80%,而高表达菌株与野生型相比胞外多糖产量增加,但致病力降低。本研究为进一步研究该基因在水稻条斑病菌中胞外多糖代谢途径的作用奠定了基础,并为利用基因工程手段改造高产黄原胶菌种提供了一种可行的策略。

关键词: 水稻细菌性条斑病菌, UDP-脱氢酶基因, Tn5 插入突变体, 胞外多糖, 致病力

Abstract: Xanthomonas oryzae pv. oryzicola (Xoc) is an important plant pathogen, which caused rice bacterial leaf blight and seriously decreased the production of rice. A mutation in putative udgH gene of Xoc leads to serious weakening on extracellular polysaccharide production and virulence, and lower extracellular amylase and cellulase activity. The phenotype of complementary strain can be restored to wild-type levels, while virulence only by 80%, and the extracellular polysaccharide production of high expression strain is increased, while the virulence is decreased. The research laid solid foundation for further study the role of the gene in extracellular polysaccharide metabolic pathways of Xoc, and provided a feasible method for constructing high-yielding strains of xanthan gum useing genetic engineering.

Key words: Xanthomonas oryzae pv. oryzicola, UDP-dehydrogenase gene, Tn5-tagged mutant, extracellular polysaccharide, virulence

中图分类号: 

  • Q93
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[1] 黄胜, 卢烨, 朱晓琳, 潘俊霞, 何勇强, 姜伟. 水稻细菌性条斑病菌一个新基因与致病相关[J]. 广西师范大学学报（自然科学版）, 2014, 32(2): 137-142.
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