广西师范大学学报（自然科学版） ›› 2014, Vol. 32 ›› Issue (2): 175-180.

• • 上一篇    

鳜鱼Siniperca chuatsi红肌sMyHC1基因cDNA的克隆及其表达分析

易潭1,2, 刘希良2,3, 宾石玉3, 王开卓2, 吴萍2, 褚武英2, 陈韬1   

  1. 1. 湖南农业大学动物医学院,湖南长沙410128;
    2. 长沙大学生物工程与环境科学系,湖南长沙410003;
    3.广西师范大学生命科学学院,广西桂林541004
  • 收稿日期:2014-03-10 出版日期:2014-06-25 发布日期:2018-09-25
  • 通讯作者: 陈韬(1969—),男,湖南长沙人,湖南农业大学教授,博士。E-mail:chentao_114@163.com
  • 基金资助:
    国家自然科学基金资助项目(31340054);湖南省自然科学基金资助项目(14JJ2135)

Molecular Cloning and Expression Analysis of Slow Myosin Heavy Chain 1 (sMyHC1) Gene cDNA in Mandarin Fish(Siniperca chuatsi)

YI Tan1,2 , LIU Xi-liang2,3, BIN Shi-yu3, WANG Kai-zhuo2, WU Ping2, CHU Wu-ying2, CHEN Tao1   

  1. 1.College of Veterinary Medicine,Hunan Agricultural University, Changsha Hunan 410128,China;
    2. College of Biological Engineering and Environmental Science, Changsha University,Changsha Hunan 410003,China;
    3.College of Life Science, Guangxi Normal University, Guilin Guangxi 541004, China
  • Received:2014-03-10 Online:2014-06-25 Published:2018-09-25

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摘要： 本研究采用同源克隆技术克隆鳜鱼红肌肌球蛋白重链1(sMyHC1)基因cDNA序列,其总长为5 940 bp。经生物信息学分析表明,鳜鱼sMyHC1基因cDNA编码区全长5 823 bp,总共编码1 940个氨基酸。序列分析表明,鳜鱼MyHC1基因cDNA存在3个ATP结合位点、3个actin结合位点、1个ELC结合位点、1个RLC结合位点和2个Loop环。对鳜鱼sMyHC1基因进行成鱼红、白肌表达分析表明,其在红肌和心肌中的表达量显著高于白肌。研究结果为深入研究鳜鱼肌纤维分型及鳜鱼肉质研究提供可靠的实验数据和理论基础。

关键词: 鳜鱼, 红肌, 肌球蛋白重链基因, 基因克隆, 序列分析

Abstract: The slow Myosin heavy chain 1 of Mandarin fish was cloned by Homologous cloning technology. The slow Myosin heavy chain 1 gene is 5 823 bp in length and encodes 1 940 amino acids. Sequence analysis showed that the slow Myosin heavy chain 1 contains three ATP binding sites,three actin binding sites, one ELC binding site,one RLC binding site and two Loops ring. The expression level in red muscle was significantly higher than that in white muscle and cardiac muscle. The above results will make contribution to provide reliable experimental data and theoretical basis for the muscle phenotype and the research of meat quility of Siniperca chuatsi.

Key words: Siniperca chuatsi, red muscle, myosin heavy chain gene, gene clone, sequence analysis

中图分类号: 

  • 753
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