广西师范大学学报(自然科学版) ›› 2016, Vol. 34 ›› Issue (2): 122-127.doi: 10.16088/j.issn.1001-6600.2016.02.018

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忍冬种质资源叶色素与光合特性分析

李建军1, 任美玲1, 王君1, 刘保彬2, 张光田3   

  1. 1.河南师范大学生命科学学院绿色药材生物技术河南省工程实验室,河南新乡453007;
    2.封丘贾庄金银花种植专业合作社,河南新乡453312;
    3.泰瑞药业有限公司,山东平邑273300
  • 收稿日期:2015-10-12 出版日期:2016-06-25 发布日期:2018-09-14
  • 通讯作者: 李建军(1964—),男,河南新乡人,河南师范大学副教授。E-mail: 043081@htu.cn
  • 基金资助:
    国家自然科学基金面上项目(31270225);中医药公共卫生专项 (财社 [2011]76号);中医药行业科研专项(201207002);河南省重点科技攻关项目(152102210288)

Analysis of Leaf Color and Photosynthetic Characteristicsof Honeysuckle Germplasm Resources

LI Jianjun1, REN Meiling1, WANG Jun1, LIU Baobin2, ZHANG Guangtian3   

  1. 1. Henan Engineering Laboratory of Biotechnologies for Medicinal Materrials,College of Life Science,Henan NormalUniversity,Xinxiang Henan 453007,China;
    2.Jiazhuang Honeysuckle Planting Professional Cooperatives in Fengqiu,Fengqiu Henan 453311,China;
    3.Tai Rui Pharmaceutial Co. Ltd., Pingyi Shandong 273300,China
  • Received:2015-10-12 Online:2016-06-25 Published:2018-09-14

摘要: 采用分光光度计和LI-6400XT光合仪对忍冬资源圃中不同种质资源叶片叶绿素、花青素含量与光合特性进行测定,并进行不同种质资源叶绿素、花青素含量与光合特性比较分析,结果表明:1、2、4、8、17号忍冬叶绿素含量较高,分别为3.70、3.65、3.04、3.07、3.52 mg/g,16号淡红忍冬最低,为1.49 mg/g;8、9号红白忍冬花青素含量较高,分别为1.113和1.360 mg/g,16号淡红忍冬为0.496 mg/g,介于忍冬和红白忍冬之间,忍冬类偏低,在0.100~0.248 mg/g;1、2、3、8、10号忍冬光合速率较高,分别为6.63、5.72、5.41、5.41、5.00 μmol·m-2s-1;光合速率与叶绿素含量呈极显著正相关,花青素相对浓度影响光合速率,光合速率与气孔导度和蒸腾速率正相关,与胞间CO2浓度显著负相关。由此可知:1、2、3号忍冬资源叶绿素含量较高,光合速率也较高,花青素含量适中;8号较9号叶绿素含量高,光合速率也高,花青素含量较低;1、2、3、8号为优质忍冬种质资源,可在生产中推广种植。

关键词: 忍冬, 种质资源, 叶绿素, 花青素, 光合速率

Abstract: Chlorophyll,anthocyanin content and photosynthetic characteristics were determined in leaves of different germplasm resources in honeysuckle resources garden by spectrophotometry and LI-6400XT photosynthetic instrument,and a comparative analysis was carried out among the chlorophyll,anthocyanin content and photosynthetic characteristics. The results showed that:the chlorophyll content of No.1 (3.70 mg/g),No.2 (3.65 mg/g),No.4 (3.04 mg/g),No.8 (3.07 mg/g) and No.17 (3.52 mg/g) were higher,the chlorophyll content of No.16 (1.49 mg/g)was the lowest. The anthocyanin content of No.8(1.113 mg/L)and No.9(1.360 mg/L)which belonged to Lonicera japonic Thunb.var.chinensis (Wats.)Bak. were higher,anthocyanin content of No.16 (0.496 mg/L) which belonges to Lonicera acuminata Wall. was between Lonicera japonica Thunb. and Lonicera japonic Thunb. var. chinensis (Wats.)Bak.,anthocyanin content of Lonicera japonica Thunb.(0.100~0.248 mg/L) was the lowest. The Photosynthetic rate of No.1 (6.63 μmol·m-2·s-1),No.2 (5.72 μmol·m-2·s-1),No.3 (5.41 μmol·m-2·s-1),No.8 (5.41 μmol·m-2·s-1) and No.10 (5.00 μmol·m-2·s-1) were higher. The photosynthetic rate was extremely and significantly correlated with chlorophyll content,anthocyanins had affect on the photosynthetic rate,photosynthetic rate was extremely significantly positive correlated with stomatal conductance and transpiration rate,but significantly negative correlated with the intercellular CO2 concentration. Conclusion: The chlorophyll content and photosynthetic rate of No.1,No.2, and No.3 honeysuckle resources were higher,the anthocyanins content of No.1,No.2,and No.3 were moderate. The chlorophyll content and photosynthetic rate of No.8 were higher than that of No.9,while the anthocyanins content was lower. Honeysuckle germplasm resources of No.1,No.2,No.3 and No.8 with good quality were suitable for popularizing in the production.

Key words: honeysuckle, germplasm resources, chlorophyll, anthocyanin, photosynthetic rate

中图分类号: 

  • R282.2
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