超敏化学发光液稀释法降低蛋白印迹的发光强度

doi:10.16088/j.issn.1001-6600.2017.02.016

广西师范大学学报（自然科学版） ›› 2017, Vol. 35 ›› Issue (2): 108-111.doi: 10.16088/j.issn.1001-6600.2017.02.016

超敏化学发光液稀释法降低蛋白印迹的发光强度

范才文, 唐娟, 罗琴, 李璐, 向秋^*

桂林医学院科学实验中心,广西桂林541004

出版日期:2017-07-25 发布日期:2018-07-25
通讯作者: 向秋(1965—),男,湖南洞口人,桂林医学院教授,博士。E-mail:guilin996@163.com
基金资助:
国家自然科学基金(81260344);广西自然科学基金(2013GXNSFAA019228);广西壮族自治区教育厅资助项目(LX2014274)

Reduced Light Intensity of Western Blot by Diluted Ultra-enhancedChemiluminescent Solution

FAN Caiwen, TANG Juan, LUO Qin, LI Lu, XIANG Qiu^*

Science Experimental Center, Guilin Medical University, Guilin Guangxi 541004, China

Online:2017-07-25 Published:2018-07-25

摘要/Abstract

摘要： 蛋白印迹(western blot)分析是根据抗原、抗体特异性结合的原理检测复杂样品中某蛋白的技术。然而当样品中的蛋白浓度过低,特别是目标蛋白为低丰度蛋白时,采用普通化学发光检测,有时出现蛋白条带发光弱,曝光不够,而采用超敏化学发光检测则出现蛋白条带发光强,曝光过度,两者的蛋白条带显影图像均不理想。本文将超敏化学发光液稀释后再检测,显影效果显著提高,蛋白条带图像清晰,可见超敏化学发光液稀释法降低蛋白印迹的发光强度,提高了蛋白条带的显影效果。

关键词: 蛋白印迹, 超敏发光液, 化学发光, 稀释

Abstract: Based on specific binding of antigen and antibody, western blot analysis is used to detect a protein in complex samples. However, using conventional chemiluminescence detection, light of the protein band is weak and X film is not much exposed, and using ultra-enhanced chemiluminescence detection,light of the protein band is strong and X film is too much exposed, both development images of protein band are not ideal sometimes, when protein concentration of the samples is too low, especially when the protein to be detected is lower abundant in X film is samples. Therefore, the protein was detected with diluted ultra-enhanced chemiluminescent solution, and the protein band was clearly showed and the development image effect of protein bands was improved obviously, which indicates that the intensity of light emission could be reduced and the development image effect of the protein bands could be improved by dilution of ultra-enhanced chemiluminescent solution in western blot analysis.

Key words: western blot, ultra-enhanced chemiluminescent solution, chemiluminescence, dilution

中图分类号:

R446.6

范才文, 唐娟, 罗琴, 李璐, 向秋. 超敏化学发光液稀释法降低蛋白印迹的发光强度[J]. 广西师范大学学报（自然科学版）, 2017, 35(2): 108-111.

FAN Caiwen, TANG Juan, LUO Qin, LI Lu, XIANG Qiu. Reduced Light Intensity of Western Blot by Diluted Ultra-enhancedChemiluminescent Solution[J]. Journal of Guangxi Normal University(Natural Science Edition), 2017, 35(2): 108-111.

参考文献

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超敏化学发光液稀释法降低蛋白印迹的发光强度

Reduced Light Intensity of Western Blot by Diluted Ultra-enhancedChemiluminescent Solution

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