广西师范大学学报（自然科学版） ›› 2017, Vol. 35 ›› Issue (1): 104-112.doi: 10.16088/j.issn.1001-6600.2017.01.017

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AMF对金橘苗根围土壤酶活性及植株生长的影响

康福丽1,2,朱国政1,2,林钰1,2,胡振兴1,2,邓荫伟1,2,冯玉能3,陈胜华3,陈付林3,刘灵1,2   

  1. 1.广西师范大学珍稀濒危动植物生态与环境保护教育部重点实验室,广西桂林541006;
    2.广西师范大学生命科学学院,广西桂林541006;
    3.广西师范大学校园管理中心,广西桂林541004
  • 出版日期:2017-01-20 发布日期:2018-07-17
  • 通讯作者: 刘灵(1968—),女,江西吉安人,广西师范大学副教授,博士。E-mail:liuling@mailbox.gxnu.edu.cn
  • 基金资助:
    广西自然科学基金(2013GXNSFAA019094);广西高校科学技术研究重点项目(ZD2014015);桂林市科学研究与技术开发计划项目(20120119-4)

Effects on Rhizospheric Soil Enzyme Activities and Growth of Fortunella margarita Seedling Inoculated by Two Arbuscular Mycorrhizal Fungi in Two Types of Soil

KANG Fuli1,2, ZHU Guozheng1,2, LIN Yu1,2,HU Zhenxing1,2, DENG Yingwei1,2, FENG Yuneng3, CHEN Shenghua3,CHEN Fulin3, LIU Ling1,2   

  1. 1.Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Normal University,Guilin Guangxi 541006,China;
    2.College of Life Science, Guangxi Normal University, Guilin Guangxi 541006,China;
    3. Campus Management Center, Guangxi Normal University, Guilin Guangxi 541004,China
  • Online:2017-01-20 Published:2018-07-17

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摘要： 为合理利用丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)资源,揭示AM真菌对金橘根围土壤酶活性和植株生长影响的机制,以喀斯特非岩溶区酸性土(AS)和岩溶区石灰土(LS)为基质进行盆栽实验,研究分别接种丛枝菌根真菌AMF的摩西球囊霉(Glomus mosseae, G.m)和幼套球囊霉(Glomus etunicatum,G.e)对金橘幼苗根围土壤酶活性的影响。结果表明:相同类型土壤中分别接种G.m和G.e后,无论何种土壤,G.e处理金橘根围菌根侵染率各指标F、m、v均显著高于G.m处理(P≤0.05),其中酸性土中接种G.e处理的最高;此外,分别接种G.e和G.m后,金橘根围土壤蛋白酶、脲酶、磷酸酶和蔗糖酶活性与土壤有机碳、有机质含量及植株生物量均比其相应的不接种对照显著增高。蛋白酶和脲酶活性变化趋势为:(LS+G.e)>(LS+G.m)>(AS+G.e)>(AS+G.m),蔗糖酶和磷酸酶活性变化则为:(AS+G.e)>(AS+G.m)>(LS+G.e)>(LS+G.m),差异均达显著水平 (P≤0.05)。G.e处理金橘根系土壤蛋白酶活性增幅最大,脲酶第二,磷酸酶最小。土壤有机质和有机碳含量变化均有(AS+G.e)>(LS+G.e)>(LS+G.m)>(AS+G.m)的变化趋势,G.e处理均显著高于G.m处理(P≤0.05)。不论酸性土或石灰土,金橘干物质积累表现为接种G.e处理显著高于G.m处理,表现出与AM菌根侵染率相似的变化趋势。可见,AMF可通过增强金橘根围土壤酶活性,影响土壤中有效养分的转化和土壤肥力,最终促进金橘生长。G.e为筛选出的能与喀斯特地区石灰土和酸性土中生长的金橘共生良好的优势菌种,具有较好的运用潜力以克服喀斯特地区土壤肥力低而导致的作物收成欠佳的难题。

关键词: 喀斯特地区;金橘;丛枝菌根真菌;土壤酶活性;植株生长

Abstract: Arbuscular mycorrhizal fungi(AMF) are ubiquitous fungi distributed widely in soil ecosystems. It has been showed that AMF play an important role in improving soil nutrition and enhancing stress resistance. In order to reasonably use AMF resources, two types of soil such as lime soil (LS) and acid soil (AS) sampling from Karst area were used as potted matrix, and two AMF including Glomus mosseae (G.m) and Glomus etunicatum (G.e) were employed to be investigated respectively. The influence mechanism of AMF on plant biomass and soil enzyme activities in rhizosphere of Fortunella margarita Seedlings of Karst area were also discussed. The results show that, comparsion to the no AMF treatment (-AM),the colonization rate of root system of Fortunella margarita seedlings inoculated with either G.e and G.m increased significantly. No matter in either type soil, the colonization frequency of root system (F), colonization rate of root segment (m) and vesicle abundance of root segment (v) of Fortunella margarita inoculated with G.e are significant higher than those with G.m. The F, m, v of Fortunella margarita seedlings with G.e in AS had the maximum level,and those with G.m in LS were second. In addition, compared with the (-AM) treatment, rhizospheric soil enzyme activities of Fortunella margarita seedlings including protease, urease,invertase and phosphatase, along with soil organic carbon (SOC) content, soil organic matter (SOM) fraction and plant biomass rose significantly. Among them, the trends of activities of protease and urease were listed: (LS+G.e)>(LS+G.m)>(AS+G.e)>(AS+G.m), those of invertase and phosphatase were: (AS+G.e)>(AS+G.m)>(LS+G.e)>(LS+G.m), with a very significant level of all (P≤0.05). Moreover, the increasement of protease and urease activity received the first and second place, respectively, the third was that of invertase, and that of phosphatase was at a minimum level. As to rhizospheric SOC content and SOM fraction of Fortunella margarita seedlings, the change trends were shown below:(AS+G.e)>(LS+G.e)>(LS+G.m)>(AS+G.m). Regardless of what type of soil, contents of SOC and SOM of Fortunella margarita inoculated with G.e treatment were significant higher than those with G.m, similar to the changing trend of the colonization rate. The dry matter accumulation (biomass)of Fortunella margarita seedlings also showed a tendency to promote growth corresponding to the AM fungi inoculation. It is concluded that AM fungal symbiont can stimulate the activities of rhizospheric soil enzyme, facilitate the conversion of available nutrients such as N, P, K and fertility in AS and LS, and promoted plant growth in the end. G.e is the screened potential dominant species which possesses a better efficiency symbiosis with Fortunella margarita seedlings no matter growing in AS and LS and have great application potential in overcoming soil infertility and poor crop output problems of Karst area.

Key words: karst area, Fortunella margarita, arbuscular mycorrhizal fungi, soil enzyme activities, growth

中图分类号: 

  • O629.9
[1] 卢峰.广西岩溶土地现状与石漠化治理模式探析[J].广西林业科学,2012,41(2):183-185.
[2] 戴明宏,张军以,王腊春,等.岩溶地区土地利用-覆被变化的水文效应研究进展[J].生态科学,2015,34(3):189-196.
[3] 魏源,王世杰,刘秀明,等.不同喀斯特小生境中土壤丛枝菌根真菌的遗传多样性[J].植物生态学报,2011,30(10):1083-1090.
[4] 王世杰,李阳兵. 喀斯特石漠化研究存在的问题与发展趋势[J].地球科学进展,2007,22(6):573-582.
[5] 罗海波,宋光煜,何腾兵,等.贵州喀斯特山区石漠化治理过程中土壤质量特性研究[J].水土保持学报,2004,18(6):112-115.
[6] 孟鹏.金柑的研究现状及其开发前景[J].农产品加工学刊,2009(11):35-37,41.
[7] 张艳.金橘胆胃丸质量标准研究[J].中国药业,2011(2):38-39.
[8] 杨清照,刘付峤,邓振权,等.金橘引种及优质丰产栽培技术[J].中国热带农业,2011(6):79-80.
[9] 魏源,王世杰,刘秀明,等.丛枝菌根真菌及在石漠化治理中的应用探讨[J].地球与环境,2012,40(1):84-91.
[10] 李爱荣,管开云.丛枝菌根养分吸收和转运机制的研究历史与进展[J].微生物学杂志,2006,26(4):72-76.
[11] 吴强盛,夏仁学,邹英宁.柑橘丛枝菌根真菌生长与根际有效磷和磷酸酶活性的相关性[J].应用生态学报,2006,17(4):685-689.
[12] 陈梅梅,陈保冬,王新军,等.不同磷水平土壤接种丛枝菌根真菌对植物生长和养分吸收的影响[J].生态学报,2009,29(4):1980-1986.
[13] 孙吉庆,刘润进,李敏.丛枝菌根真菌提高植物抗逆性的效应及其机制研究进展[J].植物生理学报,2012,48(9):845-852.
[14] PENG Sili, GUO Tao, LIU Gangcai. The effects of arbuscular mycorrhizal hyphal networks on soil aggregations of purple soil in southwest China[J].Soil Biology and Biochemistry,2013,57(57):411-417.
[15] 叶佳舒,李涛,胡亚军,等.干旱条件下AM真菌对植物生长和土壤水稳定性团聚体的影响[J].生态学报,2013,33(4):1080-1090.
[16] 王帅,王楠,张溪,等.玉米栽培模式对暗棕壤微生物学特性及养分状况的影响[J].水土保持学报,2016,30(1):165-170,195.
[17] 孙锋,赵灿灿,李江涛,等.与碳氮循环相关的土壤酶活性对施用氮磷肥的响应[J]. 环境科学学报,2014,34(4):1016-1023.
[18] 徐丽娟,刁志凯,李岩,等.菌根真菌的生理生态功能[J].应用生态学报,2012,23(1):285-292.
[19] 关松荫.土壤酶及其研究法[M].北京:农业出版社,1986.
[20] 曹慧,孙辉,杨浩,等.土壤酶活性及其对土壤质量的指示研究进展[J].应用与环境生物学报,2003,9(1):105-109.
[21] 张咏梅,周国逸,吴宁.土壤酶学的研究进展[J].热带亚热带植物学报,2004,12(1):83-90.
[22] 杨会玲,黄仁华,陈珂,等.丛枝菌根真菌(AMF)对铯胁迫宿根高粱生长及根际土壤酶的影响[J].环境化学,2015,349(4):712-717.
[23] ZHAO M, LI M, LIU R J. Effects of arbuscular mycorrhizae on microbial population and enzyme activity in replant soil used for watermelon production[J].International Journal of Engineering,Science Technology,2010,2(7):7-22.
[24] 张美庆,王幼珊,张弛,等.我国北方VA 菌根真菌某些属和种的生态分布[J].真菌学报,1994,13(3):166-172.
[25] 宋会兴,钟章成.接种幼套球囊霉对干旱生境中构树幼苗根系形态的影响[J].林业科学研究,2007,20(1):79-83.
[26] TROUVELOT A, KOUGH J L. Mesure du taux de mycorhization VA d’un systeme radiculaire. Recherche des methodes d’estimation ayantune signification fonctionnelle[M]//GIANINAZZI-PEARSON V, GIANINAZZI S. The Mycorrhizae: Physiology and Genetics. Paris:INRA Presse,1986:217-221.
[27] 鲍士旦.土壤农化分析(第三版)[M].北京:中国农业出版社,2008:25-38.
[28] 陈殿波,卢建伟.低温外热重铬酸钾氧化-比色法测定脱水污泥有机碳[J].云南化工,2012,39(1):39-43.
[29] 张军以,戴明宏,王腊春,等.西南喀斯特石漠化治理植物选择与生态适应性[J].地球与环境,2015,43(3):269-278.
[30] 何跃军,钟章成.喀斯特地区植被恢复过程中适生植物的生理生态学研究进展[J].热带亚热带植物学报,2010,18(5):586-592.
[31] 张海波,梁月明,冯书珍,等.土壤类型和树种对根际土丛枝菌根真菌群落及其根系侵染率的影响[J].农业现代化研究,2016,37(1):187-194.
[32] 张燕,李娟,姚青,等.丛枝菌根真菌对水分胁迫下枇杷实生苗生长和养分吸收的影响[J].园艺学报,2012,39(4):757-762.
[33] 肖家欣,许庆龙,刘晓敏,等.4种丛枝菌根真菌对南高丛蓝莓抗旱性的影响[J].浙江大学学报(农业与生命科学版),2016,42(4):427-434.
[34] 杨皓,范明毅,李婕羚,等.喀斯特山区无籽刺梨种植基地土壤酶活性与肥力因子的关系[J].山地学报,2016,34(1):28-37.
[35] 宰学明,郝振萍,赵辉,等.丛枝菌根化滨梅苗的根际微生态环境[J].林业科学,2014,50(1):41-48.
[36] 边雪廉,赵文磊,岳中辉,等.土壤酶在农业生态系统碳、氮循环中的作用研究进展[J].中国农学通报,2016,32(4):171-178.
[37] 谭晓燕,杨润亚,薛军于,等.AM菌剂对葡萄根围土壤微生物数量及酶活性的影响[J].鲁东大学学报(自然科学版),2012,28(1):50-53.
[38] 袁丽环,闫桂琴.丛枝菌根化翅果油树幼苗根际土壤微环境[J].植物生态学报,2010,34(6):678-686.
[39] 王建,周紫燕,凌婉婷.球囊霉素相关土壤蛋白的分布及环境功能研究进展[J].应用生态学报,2016,27(2):634-642.
[40] 赵仁竹,汤洁,梁爽,等.吉林西部盐碱田土壤蔗糖酶活性和有机碳分布特征及其相关关系[J].生态环境学报,2015,24(2):244-249.
[41] 谭红妍,闫瑞瑞,闫玉春,等.不同放牧强度下温性草甸草原土壤生物性状及与地上植被的关系[J].中国农业科学2014,47(23):4658-4667.
[42] 苏永中,赵哈林.土壤有机碳储量、影响因素及其环境效应的研究进展[J].中国沙漠,2002,22(3):220-228.
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