广西师范大学学报（自然科学版） ›› 2022, Vol. 40 ›› Issue (5): 216-226.doi: 10.16088/j.issn.1001-6600.2022030310

• 综述 • 上一篇    下一篇

荧光偏振技术在生化分析检测中的研究进展

张怡雯1,2, 韦汶言1,2, 赵晶瑾1,2*   

  1. 1.珍稀濒危动植物生态与环境保护教育部重点实验室(广西师范大学), 广西 桂林 541006;
    2.广西师范大学 环境与资源学院, 广西 桂林 541006
  • 收稿日期:2022-03-03 修回日期:2022-04-19 出版日期:2022-09-25 发布日期:2022-10-18
  • 通讯作者: 赵晶瑾(1984—), 女, 湖南慈利人, 广西师范大学教授, 博士。E-mail: jzhao12@163.com
  • 基金资助:
    国家自然科学基金(21966009)

Review on the Applications of Fluorescence Polarization and Its Biochemical Analysis

ZHANG Yiwen1,2, WEI Wenyan1,2, ZHAO Jingjin1,2*   

  1. 1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin Guangxi 541006, China;
    2. College of Environment and Resources, Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2022-03-03 Revised:2022-04-19 Online:2022-09-25 Published:2022-10-18

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摘要： 荧光偏振技术是根据体系中荧光基团与检测分子结合前后的偏振信号变化,对分子间相互作用进行研究或定量检测目标分子的一种分析方法,具有灵敏度高、分析快速、操作简便和易实现高通量测定等多种优势,同时可与其他分析检测技术联用,在生化分析和疾病诊断等领域得到广泛应用。近年来,随着现代分析技术的发展,核酸和材料科学等领域的技术进步,许多具有更高分析检测性能的荧光偏振技术相继被报道。深入探究稳定性强、成本低廉以及功能多样的荧光偏振检测技术是未来研究的发展趋势。本文主要介绍近年来荧光偏振技术检测生物小分子、核酸、蛋白质、生物酶、金属离子、农药以及生化相关物质等方面的应用,并对荧光偏振技术的前景进行展望。

关键词: 荧光偏振, 生化分析, 生物分子, 信号放大, 核酸

Abstract: Fluorescence polarization (FP) is an analytical method for the study of intermolecular interaction or quantitative detection of target molecules through the changes of FP signals before and after the combination of fluorescent groups and other molecules in the system. It has significant advantages such as high sensitivity, rapid analysis, easy operation and high throughput, which can be combined with other analytical technologies, and has been widely used in the fields of biochemical analysis and disease diagnosis. In recent years, with the development of modern analytical techniques and technological advances in the fields of nucleic acid and material science, many FP assays with higher analytical performance have been reported. The trend of future researches is to deeply explore the fluorescence polarization detection technology with strong stability, low cost and various functions. This review,mainly summarize the recent advances of FP and their analytical applications in detection of biological small molecules, nucleic acids, proteins, biological enzymes, metal ions, pesticides and biochemically related substances. Moreover, the prospect for future development of FP is provided.

Key words: fluorescent polarization, biochemical analysis, biomolecules, signal amplification, nucleic acid

中图分类号: 

  • O657
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