广西师范大学学报（自然科学版） ›› 2019, Vol. 37 ›› Issue (2): 179-187.doi: 10.16088/j.issn.1001-6600.2019.02.022

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水稻黄单胞菌2个致病变种的表型和趋化性比较研究

许力丹1,2, 赵敏1,2, 李美霖1,2, 曾晨1,2, 莫琇羽2, 刘美娜2, 朱平川1,2, 何勇强1,2*   

  1. 1.亚热带农业生物资源保护与利用国家重点实验室(广西大学),广西南宁530004;
    2.广西大学生命科学与技术学院,广西南宁530004
  • 收稿日期:2018-05-03 出版日期:2019-04-25 发布日期:2019-04-28
  • 通讯作者: 何勇强(1963—),男,河南南阳人,广西大学教授,博士。E-mail:yqhe@gxu.edu.cn
  • 基金资助:
    国家自然科学基金(31660505);国家重点研发计划(2018YFD02003021);农业部行业公益专项(201303015)

Comparative Study on Basic Phenotypes and Chemotaxis of the Two Pathovars of Xanthomonas oryzae

XU Lidan1,2, ZHAO Min1,2, LI Meilin1,2, ZENG Chen1,2, MO Xiuyu2, LIU Meina2, ZHU Pingchuan1,2, HE Yongqiang1,2*   

  1. 1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning Guangxi 530004, China;
    2.College of Life Science and Technology, Guangxi University, Nanning Guangxi 530004, China
  • Received:2018-05-03 Online:2019-04-25 Published:2019-04-28

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摘要： 水稻白叶枯病菌和水稻条斑病菌是水稻黄单胞菌Xanthomonas oryzae种下的2个致病变种,其侵染水稻的途径、定殖部位和引起的症状明显不同。本文采用平板法和毛细管法分别对2个病菌的基本表型和趋化性进行比较分析,并采用剪叶接种法和压渗接种法,检测了它们在水稻日本晴Oryza sativa L. japonica品种上的致病性。结果表明,2个病菌在部分检测指标上保持一致,均能形成隆起的黄色菌落,能合成相当数量的胞外多糖和胞外淀粉酶,对蔗糖、多种氨基酸、水稻提取物的趋化反应一致。然而,两菌在胞外蛋白酶和纤维素酶分泌、运动性,以及对葡萄糖、木糖、果糖、柠檬酸、多种植物激素趋性方面存在明显差异。

关键词: 水稻白叶枯病菌, 水稻条斑病菌, 表型, 趋化性, 致病性

Abstract: Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola are two pathovars of Xanthomonas oryzae. They have distinct differences in invasion paths, colonization niches in host plant, and disease symptoms. In this paper, the basic phenotype and chemotaxis of the two kinds of bacteria were analyzed by plate method and capillary method respectively, and the pathogenicity on the rice Oryza sativa L. japonica cultivar Nipponbare was tested by using the method of leaf-cutting inoculation and pressure infiltration inoculation. The results showed that the two kinds of bacteria were consistent in some indexes tested. They could form the yellow colony, and synthesize a considerable amount of extracellular polysaccharide and extracellular amylase. They also share similar chemotactic reactions to sucrose, amino acids and rice extracts. However, there are significant differences between the two bacteria in secreting extracellular protease and cellulase, and the chemotactic responses to glucose, xylose, fructose, citric acid, and many kinds of plant hormones.

Key words: Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola, phenotypes, chemotaxis, pathogenicity

中图分类号: 

  • S432.1
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[1] 姜伟, 吴德波, 林茵, 杨文珊, 倪哲, 何勇强, 黄胜. 水稻白叶枯病菌致病相关基因筛选系统的建立[J]. 广西师范大学学报（自然科学版）, 2014, 32(4): 135-141.
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