广西师范大学学报（自然科学版） ›› 2012, Vol. 30 ›› Issue (4): 59-66.

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金铂纳米合金催化磷钼蓝光度法测定半胱氨酸

蒋治良1,2, 姚东梅1,2, 韦燕燕1,2   

  1. 1.广西师范大学广西环境污染控制理论与技术重点实验室,广西桂林 541004;
    2.广西师范大学环境与资源学院,广西桂林 541004
  • 收稿日期:2012-01-23 发布日期:2018-11-27
  • 通讯作者: 蒋治良(1965—),男,广西桂林人,广西师范大学教授,博导。E-mail:zljiang@mailbox.gxnu.edu.cn
  • 基金资助:
    国家自然科学基金资助项目(21267004,21075023);广西环境污染控制理论与技术重点实验室基金资助项目

AuPt Nanoalloy Catalytic Phosphomolybdate Blue Spectrophotometric Assay for Cysteine

JIANG Zhi-liang1,2, YAO Dong-mei1,2, WEI Yan-yan1,2   

  1. 1.Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology,Guangxi Normal University,Guilin Guangxi 541004,China;
    2.College of Environmental and Resource,Guangxi Normal University,Guilin Guangxi 541004,China
  • Received:2012-01-23 Published:2018-11-27

摘要： 在pH2.8 HCOOH-HCOONa缓冲溶液中,半胱氨酸(Cys)可与金铂纳米粒子(AuPtNP)作用发生聚集,导致深红色的金铂纳米微粒变成蓝紫色。AuPtNP对甲酸还原磷钼酸生成磷钼蓝反应具有较强的催化作用,而金铂纳米粒子聚集体(AuPtNPA)的催化作用较弱。随着Cys浓度增大,体系中AuPtNPA增多,而AuPtNP减少,催化能力随之减弱,磷钼蓝在700 nm处的吸光度值降低,Cys浓度在30～350 nmol/L与其吸光度降低值ΔA呈良好的线性关系,检测限为28 nmol/L,表观摩尔吸光系数为1.8×106 L·mol-1·cm-1。据此建立了测定半胱氨酸的纳米金铂催化光度分析新方法。

关键词: 半胱氨酸, 纳米合金催化, 磷钼蓝反应, 分光光度法

Abstract: In the pH 2.8 HCOOH-HCOONa buffer solution,the cysteine(Cys) interacted with gold-platinum nanoparticles (AuPtNP) to form aggregations that resulted in its color changing from wine red to blue-violet.The AuPtNP has a strong catalysis on the phosphomolybdate blue reaction of formic acid-phosphomolybdic acid,but the AuPtNP aggregations (AuPtNPA) catalysis is weak.With the increase of Cys concentration,the AuPtNPA increased and the AuPtNP decreased,the catalysisdecreased and the absorption value of phosphomolybdate blue at 700 nm decreased.The Cys concentrations in the range of 30～350 nmol/L had good linear response to the decreased absorption value ΔA,with a detection limit of 28 nmol/L,andits molar absorption coefficients was 1.8×106 L·mol-1·cm-1.Thus,anew nanocatalytic spectrophotometric method is established for the determination of Cys.

Key words: cysteine, nanoalloy catalyst, phosphomolybdate bluereaction, spectrophotometry

中图分类号: 

  • O657.32
[1] YANG Xiao-feng,LIU Ping,WANG Li-ping,et al.A Chemosensing ensemble for the detection of cysteine based on the inner filter effect using a rhodamine B spirolactam[J].J Fluoresc,2008,18:453-459.
[2] STIPANUK M H,Jr DOMINY J E,LEE J I,et al.Mammalian cysteine metabolism:new insights into regulation of cysteine metabolism[J].J Nutr,2006,136:1652S-1659S.
[3] 史伯安,郗娟,何治柯.流动注射-化学发光法测定半胱氨酸[J].分析测试学报,2005,24(6):102-105.
[4] LAU C,QIN Xiao-jin,LIANG Jian-ying,et al.Determination of cysteine in apharmaceutical formulation by flow injection analysis with a chemiluminescence detector[J].Anal Chim Acta,2004,514:45-49.
[5] SHANG Li,DONG Shao-jun.Sensitive detection of cysteine based on fluorescent silver clusters[J].Biosens Bioelectron,2009,24:1569-1573.
[6] HUANG Shan,XIAO Qi,LI Ran,et al.A simple and sensitive methodfor L-cysteine detection based on the fluorescence intensity increment of quantum dots[J].Anal Chim Acta,2009,645:73-78.
[7] WHITE P C,LAWRENCE N S,TSAI Y C,et al.Electrochemically drivenderivatisation-detection of cysteine[J].Mikrochim Acta,2001,137:87-91.
[8] ZHAO Chuan,ZHANG Jun-cai,SONG Jun-feng.Determination of L-cysteine in amino acid mixture and human urine by flow-injection analysis with a biamperometric detector[J].Anal Biochem,2001,297:170-176.
[9] NEKRASSOVA O,LAWRENCE N S,COMPTON R G.Selective electroanalytical assay for cysteine at a boron doped diamond electrode[J].Electroanalysis,2004,16:1285-1291.
[10] LIU Xiao,LUO Li-qiang,DING Ya-ping,et al.Simultaneous determination of L-cysteine and L-tyrosine using Au-nanoparticles/poly-eriochrome blackT film modified glassy carbon electrode[J].Bioelectrochemistry,2012,86:38-45.
[11] WANG Lai-hao,HUANG Wen-shuan.Electrochemical oxidation of cysteine at a film gold modified carbon fiber microelectrode its application in a flow-throughvoltammetric sensor[J].Sensors,2012,12:3562-3577.
[12] ATTA N F,GALAL A,EL-ADS E H.A novel sensor of cysteine self-assembled monolayers over gold nanoparticles for the selective determination of epinephrinein presence of sodium dodecyl sulfate[J].Analyst,2012,137(11):2658-2668.
[13] CHEN Zhang,LUO Sheng-lian,LIU Cheng-bin,et al.Simple and sensitive colorimetric detection of cysteine based on ssDNA-stabilized gold nanoparticles[J].Anal Bioanal Chem,2009,395:489-494.
[14] WU Tong,LI Yuan-fang,HUANG Cheng-zhi.Selectively colorimetric detectionof cysteine with triangular silver nanoprisms[J].Chin Chem Lett,2009,20:611-614.
[15] WEI Xiao-yi,QI Li,TAN Jun-jun,et al.A colorimetric sensor for determination of cysteine by carboxymethyl cellulose-functionalized gold nanoparticles[J].Analytica Chimica Acta,2010,671(1/2):80-84.
[16] RAFII M,ELANGO R,COURTNEY-MARTIN G,et al.High-throughput and simultaneous measure-ment of homocysteine and cysteine in human plasma and urine by liquidchromatography-electrospray tandem mass spectrometry[J].Anal Biochem,2007,371:71-81.
[17] JIN Wen-rui,WANG Yan.Determination of cysteine by capillary zone electrophoresis with end-column amperometric detection at a gold/mercury amalgam microelectrode without deoxygenation[J].J Chromatograph A,1997,769:307-314.
[18] NEZAMZADEH E A,HASHEMI H S.Voltammetric determination of cysteine using carbon paste electrode modified with Co(Ⅱ)-Y zeolite[J].Talanta,2012,88:201-208.
[19] PEREVEZENTSEVA D O,GORCHAKOV E V.Voltammetric determination ofcysteine at a graphite electrode modified with gold nanoparticles[J].J Solid State Electrochem,2012,16(7):2405-2410.
[20] NEKRASSOVA O,LAWRENCE N S,COMPTON R G.Analytical determinationof homocysteine:a review[J].Talanta,2003,60:1085-1095.
[21] HAUGLAND R P.Handbook of fluorescent probes and research products[M].Eugene:Molecular Probes,2002:79-98.
[22] BURDA C,CHEN Xiao-bo,NARAYANAN R,et al.Chemistry and properties of nanocrystals of different shapes[J].Chem Rev,2005,105:1025-1102.
[23] PLASMONICS O E.Merging photonics and electronics at nanoscale dimensions[J].Science,2006,311:189-193.
[24] MO Zhi-rong,ZHAO Fa-qiong,XIAO Fei,et al.Preparation and characterization of AuPt alloy nanoparticle-multi-walled carbon nanotube-ionic liquid composite film for electrocatalytic oxidation of cysteine[J].J Solid State Electrochem,2010,14:1615-1620.
[25] MONTERO M A,GENNERO DE CHIALVO M R,CHIALVO A C.Electrocatalytic activityof core-shell Au/Pt nanoparticles for the hydrogen oxidation reaction[J].Int J Hydrogen Energy,2011,36:3811-3816.
[26] MIHUT C,DESCORME C,DUPREZ D,et al.Kinetic and spectroscopic characterization of cluster-derived supported Pt-Au catalysts[J].J Catalysis,2002,212:125-135.
[27] HABRIOUX A,SIBERT E,SERVAT K,et al.Activity of platinum-goldalloys forglucose electrooxidation in biofuel cells[J].J Phys Chem B,2007,111:10329-10333.
[28] YANG Lei,CHEN Jin-hua,ZHONG Xin-xian,et al.Au/Pt nanoparticles preparedby one-phase protocol and their electrocatalytic properties for methanol oxidation[J].Colloids and Surfaces A:Physicochem Eng Aspects,2007,295:21-26.
[29] 唐美玲,梁爱惠,刘庆业,等.纳米催化还原Ni(Ⅱ)体系光度法测定痕量钯[J].广西师范大学学报:自然科学版,2011,29(3):47-51.
[30] LI Qian-qian,ZHANG Li-juan,LI Jin-ge,et al.Nanomaterial-amplified chemiluminescence systems and their applications in bioassays[J].Tr Anal Chem,2011,30(2):401-413.
[31] JIANG Zhi-liang,ZHOU Lian-ping,LIANG Ai-hui.Resonance scattering detection of trace melamine using aptamer-modified nanosilver probe as catalyst without separation of its aggregations[J].Chem Comm,2011,47:3162-3164.
[32] LIANG Ai-hui,ZHANG Yi,FAN Yan-yan,et al.Catalysis of aptamer-modified AuPd nanoalloy probe and its application to resonance scattering detection of trace UO2+2[J].Nanoscale,2011,3(8):3178-3184.
[33] 李正平,段新瑞,白玉惠.基于金纳米粒子自组装的分光光度法测定半胱氨酸[J].分析化学,2006,34(8):1149-1152.
[34] WANG Hong,WANG Wei-sheng,ZHANG Hua-shan.Spectrofluorimetic determinationof cysteine based on the fluorescence inhibition of Cd(Ⅱ)-8-hydroxyquinoline-5-sulphonic acid complex by cysteine[J].Talanta,2001,53:1015-1019.
[35] CHWATKO G,BALD E.Determination of cysteine in human plasma by high-performance liquid chromatography and ultraviolet detection after pre-column derivatization with 2-chloro-1-methylpyridinium iodide[J].Talanta,2000,52:509-515.
[36] ZEN Jyh-myng,KUMAR A S,CHEN Jyh-cheng.Electrocatalytic oxidation and sensitive detection of cysteine on a lead ruthenate pyrochlore modified electrode[J].Anal Chem,2001,73:1169-1175.
[37] DENG Chun-yan,CHEN Jin-hua,CHEN Xiao-li,et al.Electrochemical detectionof L-cysteine using a boron-doped carbon nanotube-modified electrode[J].Electrochimica Acta,2009,54:3298-3302.
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