吉富罗非鱼HSP70基因的多态性及其与耐寒性状的相关性研究

doi:10.16088/j.issn.1001-6600.2019.01.025

广西师范大学学报（自然科学版） ›› 2019, Vol. 37 ›› Issue (1): 211-217.doi: 10.16088/j.issn.1001-6600.2019.01.025

• 第二十四届全国信息检索学术会议专栏 • 上一篇下一篇

吉富罗非鱼HSP70基因的多态性及其与耐寒性状的相关性研究

宾石玉¹, 钟丹丹¹, 杜雪松², 张永德², 林勇^2*, 黄姻², 文露婷²

1. 广西师范大学生命科学学院/珍稀濒危动植物生态与环境保护教育部重点实验室,广西桂林541006;
2. 广西水产科学研究院广西水产遗传育种与健康养殖重点实验室,广西南宁530021

收稿日期:2018-06-16 出版日期:2019-01-20 发布日期:2019-01-08
通讯作者: 林勇(1969—),男,广西玉林人,广西水产科学研究院研究员。E-mail:linnn2005@126.com
基金资助:
广西科学研究与技术开发计划(桂科AB16380074);国家现代农业产业技术体系广西罗非鱼创新团队建设项目(nycytxgxcxtd-08-01); 广西直属公益性科研院所基本科研业务费专项资金(GXIF-2014-016)

Correlation Analysis of Polymorphism and Its Cold Tolerance Traits HSP70 Gene of GIFT Tilapia (Oreochromis niloticus)

BIN Shiyu¹, ZHONG Dandan¹, DU Xuesong²,ZHANG Yongde², LIN Yong^2*, HUANG Yin²,WEN Luting²

1.College of Life Science, Guangxi Normal University/Key Laboratory of Ecology of Rare and Endangered Speicies and Environmental Protection, Ministsry of Education, Guilin Guangxi 541006,China;
2.Guangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning Guangxi 530021,China

Received:2018-06-16 Online:2019-01-20 Published:2019-01-08

摘要/Abstract

摘要： 利用PCR-SSCP技术对吉富罗非鱼HSP70基因多态性进行检测,并采用最小二乘法分析多态性与罗非鱼Oreochromis niloticus耐寒性状的相关性。结果发现,依据HSP70基因序列设计的引物HP70-1、HP70-4、HP70-6不同耐寒能力样本中的电泳图具有带型多态性,且该基因多态性与其耐寒性状显著相关。据此推断HSP70基因是潜在的罗非鱼耐寒相关基因。

关键词: 吉富罗非鱼, 耐寒性状, 多态性, PCR-SSCP, HSP70基因

Abstract: The polymorphisms of HSP70 in the GIFT tilapia samples were detected by using PCR-SSCP technique and the correlation with cold tolerance capability of GIFT tilapia was analyzed by least square method in this study. The results showed that the electrophoresis diagram of the primer HP70-1, HP70-4, HP70-6 designed according to the HSP70 has the polymorphism of the belt type in different cold tolerance samples. The polymorphism of the gene was significantly correlated with its cold tolerance. Therefore, it is inferred that HSP70 genes are potential cold-resistant genes of tilapia.

Key words: GIFT tilapia (Oreochromis niloticus), cold tolerance traits, polymorphism, PCR-SSCR, HSP70 gene

中图分类号:

Q953.3

宾石玉, 钟丹丹, 杜雪松, 张永德, 林勇, 黄姻, 文露婷. 吉富罗非鱼HSP70基因的多态性及其与耐寒性状的相关性研究[J]. 广西师范大学学报（自然科学版）, 2019, 37(1): 211-217.

BIN Shiyu, ZHONG Dandan, DU Xuesong,ZHANG Yongde, LIN Yong, HUANG Yin,WEN Luting. Correlation Analysis of Polymorphism and Its Cold Tolerance Traits HSP70 Gene of GIFT Tilapia (Oreochromis niloticus)[J]. Journal of Guangxi Normal University(Natural Science Edition), 2019, 37(1): 211-217.

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吉富罗非鱼HSP70基因的多态性及其与耐寒性状的相关性研究

Correlation Analysis of Polymorphism and Its Cold Tolerance Traits HSP70 Gene of GIFT Tilapia (Oreochromis niloticus)

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