广西师范大学学报（自然科学版） ›› 2018, Vol. 36 ›› Issue (1): 112-120.doi: 10.16088/j.issn.1001-6600.2018.01.016

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Fe3O4/氧化石墨烯复合纳米粒子的制备及其体外毒性评价

毛芳芳*,庞锦英,李建鸣,陆春谊   

  1. 广西师范学院化学与材料学院,广西南宁530001
  • 收稿日期:2017-05-05 出版日期:2018-01-20 发布日期:2018-07-17
  • 通讯作者: 毛芳芳(1990—),女,福建永安人,广西师范学院教师。E-mail:675758721@qq.com
  • 基金资助:
    国家国际科技合作专项(2013DFA51290)

Facile Solvothermal Synthesis and Biocompatible Evaluation of Fe3O4/Graphene Oxide Magnetic Nanocomposites in vitro

MAO Fangfang*,PANG Jinying,LI Jianming,LU Chunyi   

  1. College of Chemistry and Materials Science,Guangxi Teachers Education University,Nanning Guangxi 530001,China
  • Received:2017-05-05 Online:2018-01-20 Published:2018-07-17

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摘要： 用溶剂热法制备得到Fe3O4/氧化石墨烯复合纳米粒子,并对其生物相容性进行研究。使用X射线粉末衍射仪(XRD)、扫描电子显微镜(SEM)等分析仪器对其进行表征,并通过一系列溶血试验及MTT细胞毒性试验对复合纳米粒子的体外毒性进行评价。结果显示,当复合纳米粒子浓度小于5 g/L时,溶血率低于5.00%;当复合纳米粒子浓度不高于0.25 g/L、孵育时间不长于72 h时,细胞相对增殖率大于75%,属0~1级,认为此范围浓度材料没有细胞毒性。从目前的实验结果来看制备所得Fe3O4/氧化石墨烯复合纳米粒子具有良好的生物相容性,在生物医学方面具有潜在的应用前景。

关键词: 氧化石墨烯, Fe3O4纳米粒子, 生物相容性, 细胞毒性, 血液相容性

Abstract: Fe3O4/graphene oxide (Fe3O4/GO) nanocomposite was synthesized by solvothermal strategy. The effects of reagent ratio and reaction time on the morphology and diameters of nanocomposites were investigated. They were characterized using X-ray diffraction,scanning electron microscopy,thermogravimetric analysis,and Fourier-transform infrared spectroscopy. Cytotoxicity test and hemolysis assay were investigated to assess their toxicity in vitro and to explore their potential application in the biomedical fields. The toxicity of Fe3O4/GO nanoparticles in mouse fibroblast (L-929) cell lines is between grade 0 to grade 1,and the material lacks hemolysis activity.

Key words: graphene oxide;Fe3O4 nanoparticles;biocompatibility;cytotoxicity;hemocompatibility

中图分类号: 

  • TB31
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