广西师范大学学报（自然科学版） ›› 2016, Vol. 34 ›› Issue (1): 162-167.doi: 10.16088/j.issn.1001-6600.2016.01.026

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应激诱导小鼠杏仁核theta波段下调介导焦虑反应

姜方洁1,2, 付玉3, 吴桂生1, 杨丽萍1, 2,4, 唐贺1,2, 邓科1, 林万华1, 5, 罗怀容1   

  1. 1.中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南昆明650201;
    2.中国科学院大学生命科学院,北京100039;
    3.昆明理工大学基础医学院,云南昆明650093;
    4.云南省中医中药研究所中药研究室,云南昆明650223;
    5.广西师范大学生命科学学院,广西桂林541006
  • 收稿日期:2015-05-05 发布日期:2018-09-14
  • 通讯作者: 罗怀容(1975—),女,四川泸县人,中国科学院昆明植物研究所研究员,博士。E-mail:luohuairong@mail.kib.ac.cn
  • 基金资助:
    国家自然科学基金资助项目(81370453);云南省自然科学基金资助项目(2012FB181);云南省重点新产品开发计划项目基金资助(2014BC011)

Chronic Stress Affects the Theta Activity in BLAto Induce the Stress Response in Mice

JIANGFangjie1,2, FU Yu3, WU Guisheng1, YANG Liping 1, 2,4, TANG He1,2, DENG Ke1, LIN Wanhua1,5, LUO Huairong1   

  1. 1.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming Yunnan 650201,China;
    2.University of Chinese Academy of Sciences, Beijing 100039,China;
    3.Kunming University of Science and Technology, Kunming Yunnan 650093,China;
    4.Yunnan Institute of Traditional Chinese Medicine and Materia Medica, Kunming Yunnan 650223,China;
    5. College of Life Science,Guangxi Normal University, Guilin Guangxi 541006, China
  • Received:2015-05-05 Published:2018-09-14

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摘要： 慢性应激能引发诸多情绪行为的改变,诱导产生广义焦虑症、抑郁症、双极紊乱等情绪障碍性疾病。慢性应激首先引起焦虑情绪处理相关核团前额叶-杏仁核-腹侧海马区神经活动的功能性的改变,然后导致这些核团的结构发生改变。慢性应激造成杏仁核基底外侧核的锥体神经元的树突脊数量增多,谷氨酸能神经元的兴奋性投射增强,γ-氨基丁酸能神经元的抑制性投射减弱,导致焦虑样行为症状。由于慢性应激对于这些参与情绪调控的关键核团的神经活动的功能改变尚不明确,所以我们建立小鼠慢性应激的模型,分别在对照鼠和模型鼠的杏仁核基底外侧核及海马CA3区记录局部场电位,分离局部场电位的alpha、 beta、 delta、 theta、 gamma波段,分析其局部场电位各个波段的绝对功率和相对功率的差异。结果显示:慢性应激造成小鼠杏仁核基底外侧核的局部场电位在5个脑电波段的绝对功率均具有上调趋势,其中delta波段上调显著;进一步分析局部场电位的相对功率发现,慢性应激造成delta波段的相对功率显著上调,而theta波段的相对功率显著下调。

关键词: 慢性应激, 杏仁核基底外侧核, 海马CA3区, 局部场电位

Abstract: Chronic exposure to stress triggered many dysfunctional emotional behaviors, like generalized anxiety disorder, depression and bipolar disorder. Prolonged stress led to structural changes in the prefrontal cortex-amygdala-ventral hippocampus emotional circuit and thus exerted anxiety behavioral phenotypes. But the alteration of neural activity for those nucleus that involved in emotional regulation is not clear. So chronic stress model in mice was established local field potential (LFP) were recorded from the basolateral amygdala (BLA) and hippocampal CA3 respectively in freely exploring mouse and separated the signals into alpha, beta, and delta, theta, gamma frequency bands. In contrast to the control groups, in BLA, stress induced a trend of increase of LFP absolute power at these five bands, the increase at delta band was significant. Through further analysis of the LFP relative power, it showed that there was a distinct increase at delta band and a significant decrease at theta band in stressed mice.

Key words: chronic stress,, BLA, CA3, local field potential

中图分类号: 

  • R338.8
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