广西师范大学学报（自然科学版） ›› 2015, Vol. 33 ›› Issue (3): 98-103.doi: 10.16088/j.issn.1001-6600.2015.03.015

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藏药多刺绿绒蒿超临界CO2萃取及其成分研究

曾擎屹1,2,3, 刘小翠1,2,3, 白雪1,2,3, 林鹏程1,2,3, 吉守祥1,2   

  1. 1. 青海民族大学药学院,青海西宁810007;
    2.青海民族大学青海省青藏高原植物资源化学研究重点实验室,青海西宁810007;
    3. 青海民族大学青海省药物分析重点实验室,青海西宁810007
  • 收稿日期:2015-03-18 出版日期:2015-05-10 发布日期:2018-09-20
  • 通讯作者: 林鹏程(1966—),男,青海西宁人,青海民族大学教授,博导。E-mail: qhlpc@126.com
  • 基金资助:
    国家自然科学基金资助项目(81160554)

Composition of Meconopsis horridula Hook.f. & Thomson in SCF-CO2 Extraction

ZENG Qing-yi1,2,3, LIU Xiao-cui1,2,3, BAI Xue1,2,3, LIN Peng-cheng1,2,3, JI Shou-xiang1,2   

  1. 1. College of Pharmacy,Qinghai University for Nationalities,Xining Qinghai 810007, China;
    2. Key Laboratory of PlantResources of Qinghai-Tibet Plateau in Chemical Research, Qinghai University for Nationalities, Xining Qinghai 810007,China;
    3. Key Laboratory of Pharmaceutical Analysis in Qinghai Province, Xining Qinghai 810007, China
  • Received:2015-03-18 Online:2015-05-10 Published:2018-09-20

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摘要： 本文以多刺绿绒蒿为原料,研究超临界CO2萃取多刺绿绒蒿的最佳工艺及其萃取物的化学成分。采用均匀设计方法考察萃取时间、压力、温度等影响因素,得到超临界CO2萃取多刺绿绒蒿的最佳工艺条件,并对萃取物结合GC-MS保留指数进行分析。本文得出最佳萃取工艺:萃取时间180 min,萃取压力28 MPa,萃取温度45 ℃。从萃取物中分离出55个可识别峰,结合Kovats 保留指数共鉴定出53个成分,占总成分的80.58%,其中主要成分为γ-谷甾醇、亚油酸、棕榈酸。

关键词: 多刺绿绒蒿, 超临界CO2, 均匀设计, GC-MS, Kovats保留指数

Abstract: Meconopsis horridula Hook.f. & Thomson was chosen as raw materials to study the optimum parameters of SCF-CO2 extraction of Meconopsis horridula Hook.f. & Thomson and chemical constituents of extractive matters. SCF-CO2 extraction condition was selected by uniform design. In the experiment, three factors should be taken into consideration, such us the extraction time, extraction pressure and extraction temperature. GC-MS was applied to analyze the extraction. The optimum condition were obtained: the extraction time was 180 minutes,the extraction pressure was 28 MPa and the extraction temperature was 45 ℃. 55 compounds in Meconopsis horridula Hook.f. & Thomson were detected by GC-MS and 53 compounds were identified by MS combined with Kovats retention index. The major constituents of the extractive matters were clionasterol, linoleic acid and palmic acid.

Key words: Meconopsis horridula Hook.f. &, Thomson, SCF-CO2, uniform design, GC-MS, Kovats

中图分类号: 

  • O415.3
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