碱脱胶法优化提取蚕丝素蛋白及其构象光谱

广西师范大学学报（自然科学版） ›› 2014, Vol. 32 ›› Issue (2): 88-94.

碱脱胶法优化提取蚕丝素蛋白及其构象光谱

程蕾^1,2, 辛伟彪¹, 刘祉序¹, 王卓渊¹, 沈星灿¹

1. 广西师范大学药用资源化学与药物分子工程教育部重点实验室,广西桂林541004;
2. 广西师范大学科技处,广西桂林541004

收稿日期:2013-10-11 出版日期:2014-06-25 发布日期:2018-09-25
通讯作者: 沈星灿(1974—),女,湖南邵阳人,广西师范大学教授,博士。E-mail: xcshen@mailbox.gxnu.edu.cn
基金资助:
国家自然科学基金资助项目(21161003,21364002);广西自然科学基金重点资助项目(2012GXNSFDA053007);广西药用资源化学与药物分子工程重点实验室基金资助项目(CMEMR2012-A12);广西医药产业人才小高地项目资助

Optimization of Silk Fibroin Extraction by Alkali Degumming and Spectra of Its Conformation in Solution

CHENG Lei^1,2, XIN Wei-biao¹, LIU Zhi-xu¹, WANG Zhuo-yuan¹, SHEN Xing-can¹

1. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, Guangxi Normal University, Guilin Guangxi 541004, China;
2. Science and Technology Department, Guangxi Normal University, Guilin Guangxi 541004, China

Received:2013-10-11 Online:2014-06-25 Published:2018-09-25

摘要/Abstract

摘要： 采取碱脱胶法对广西产桑蚕茧脱胶条件进行优化,利用苦味酸胭脂红法和扫描电镜对蚕丝脱胶程度进行检测,结果表明,蚕丝在脱胶浴比为1:200,脱胶30 min的条件下,其表面丝胶可脱除效果最好。将蚕丝脱胶所得丝素蛋白分别溶解在CaCl₂-EtOH-H₂O、LiBr-H₂O和LiBr-EtOH-H₂O 3种溶剂中获再生蚕丝素蛋白溶液。在相同条件下,采用红外光谱、拉曼光谱对比研究蚕丝素蛋白的构象,结果表明碱脱胶法得到的天然丝素蛋白以β-折叠构象为主,再生丝素蛋白溶液构象以无规卷曲为主。对比3种溶剂对蛋白构象的影响,结果表明:溶剂中Li⁺比Ca²⁺对丝素蛋白的二级结构扰动更显著,倾向破坏蚕丝素蛋白的β-折叠构象,且溶剂中加入适量乙醇会进一步促进金属离子对丝素蛋白构象的影响。

关键词: 碱脱胶法, 蚕丝素蛋白, 红外光谱, 拉曼光谱, 构象

Abstract: Degumming conditions of silkworm cocoon produced in Guangxi province were optimized by the method of alkali degumming. The degumming rates of silkworm were checked by the carmine of picric acid and SEM. It is showed that the silkworm can be degummed clearly with the condition of 30 min, two times and 1:200 of bath ratio for degumming. Silk fibroin can be prepared by dissolution and degumming of three solvents of CaCl₂-EtOH-H₂O, LiBr-H₂O and LiBr-EtOH-H₂O. The conformation of silk fibroin was characterized by the optical spectroscopy of Raman and IR. The silk is mainly the conformation of β-folding by alkali degumming. After dissolving and degumming the silk, the random coil is mainly the conformation of silk fibroin. Comparing with three dissolving systems, it is showed that alcohol is better for the formation of the conformation of the random coil for silk fibroin, and Li⁺ has more influence than Ca²⁺ for secondary structure of silk fibroin.

Key words: alkali degumming, silk fibroin, IR spectra, Raman spectra, conformation

中图分类号:

TQ321.4

程蕾, 辛伟彪, 刘祉序, 王卓渊, 沈星灿. 碱脱胶法优化提取蚕丝素蛋白及其构象光谱[J]. 广西师范大学学报（自然科学版）, 2014, 32(2): 88-94.

CHENG Lei, XIN Wei-biao, LIU Zhi-xu, WANG Zhuo-yuan, SHEN Xing-can. Optimization of Silk Fibroin Extraction by Alkali Degumming and Spectra of Its Conformation in Solution[J]. Journal of Guangxi Normal University(Natural Science Edition), 2014, 32(2): 88-94.

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