广西师范大学学报(自然科学版) ›› 2013, Vol. 31 ›› Issue (1): 97-103.

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枸骨与小果十大功劳的叶形态-脉序鉴别特征

郭敏, 廖月葵, 徐灵莺, 冼俞均, 何报作   

  1. 广西中医药大学药学院,广西南宁530001
  • 收稿日期:2012-07-31 出版日期:2013-03-20 发布日期:2018-11-26
  • 通讯作者: 何报作(1948—),男,广西南宁人,广西中医药大学教授。E-mail:hobaozuo@yahoo.com.cn
  • 基金资助:
    国家自然科学基金资助项目(30860371)

Identification Characters of Leaf Morphological and Venation Pattern of Ilex cornuta with Its Confused Herb Mahonia bodinieri

GUO Min, LIAO Yue-kui, XU Ling-ying, XIAN Yu-jun, HE Bao-zuo   

  1. Faculty of Pharmacy,Guangxi University of Chinese Medicine,Nanning Guangxi 530001,China
  • Received:2012-07-31 Online:2013-03-20 Published:2018-11-26

摘要: 为寻找枸骨叶与其易混淆品小果十大功劳叶的主要鉴别特征,建立LMVP(叶形态-脉序图谱鉴别法,结果显示:两者在叶形态和脉序方面的主要特征存在明显区别。枸骨叶的主要特征为:具短柄;类长方形、矩圆状长方形、椭圆形、卵形,偶近圆形;脉序类型为达缘-环结-半达缘混合羽状脉序、环结羽状脉序或环结-半达缘混合羽状脉序;基生二级脉≤2条;网眼多由一至三级脉围成,形状大小不一,较大,直径2~9 mm。小果十大功劳小叶的主要特征:无小叶柄;长卵形、卵状披针形、卵形或近圆形;脉序类型为环结-半达缘混合羽状脉序或半达缘羽状脉序;基生二级脉≥3条;网眼由一至四级脉围成,形状为四至六边形或三角形,大小形状较规则,较小,直径0.4~0.8 mm。以上述5组区别点为指标,鉴别结果的正确性ηac=98.2%~100% ;重复性为符合率ηaro=98.2%~99.6%,一致性强度Kappa=0.96~0.99。可见,建立的“叶形态-脉序图谱鉴别法”可准确地区别枸骨叶与小果十大功劳叶以及它们的饮片(丝)。该法具有操作简便、快速、价廉、可靠性强等特点。

关键词: 枸骨叶, 小果十大功劳叶, 叶形态, 脉序图谱, 中药鉴定

Abstract: To study the identification characters of Ilex cornuta and its confused herb Mahonia bodinieri and to establish an identification method.LMVP (leaf morphological-venation pattern for identification Chinese herbs),and QAERM (quantitatively analyze and evaluating reliability for the methodof identification Chinese herbs) were applied for the study.There exsist distinct differences between leaf morphological and venation of the two kinds.The mainfeatures ofthe leaf of Ilex cornuta:it is of short petiole with different shapes likesubrectangle,oblong-rectangle,elliptical,ovate,occasionally suborbicular.The venation shapes,such as craspedodromous-brochidodromous-semicraspedodromous pinnate,brochidodromous pinnate,and brochidodromous-semicraspedodromous pinnate,differ from the basal secondary veins numbering ≤2.The areoles are formed by veinsfrom primary vein to tertiary vein,their shapes and size were various,big-sized with a diameter about 2~9 mm.The main features of the leaflets of Mahonia bodinieri:it is of no petiolule,and the shapes are long-ovate,ovate-lanceolate,ovate or suborbicular.The venation differ from brochidodromous-semicraspedodromous pinnate to semicraspedodrom-ous pinnate with the basal secondary vein numbering ≥3.The areoles are formed by veins from primary vein to quaternary vein with different shapes like quadrilateral,pentagon,hexagon or triangle.They are regular in size and shapes,but small in size with a diameter about 0.4~0.8 mm.Withkey differences of the 5 mentioned groups,both plants and their velvetherbal pieces could be successfully identified from each other.The accuracy of identification results (Ac) was from 98.2% to 100%,the repeatability of identification results:agreement rate for observation (ARO) was from 98.2% to 99.6% and Kappa value was from 0.96 to 0.99.The established method is simple,rapid,economic and reliable.

Key words: Ilex cornuta, Mahonia bodinieri, leaf morphological, venation pattern, identification Chinese herbs

中图分类号: 

  • R282.5
[1] 谢宗万.中药材品种论述:中册[M].上海:上海科技出版社,1984:284-290.
[2] 国家药典委员会.中国药典1990年版:一部[S].北京:人民卫生出版社,1990:204.
[3] 国家药典委员会.中国药典临床用药须知:中药饮片卷:2010年版[S].北京:中国医药科技出版社,2011:368.
[4] 刘振启,刘杰.枸骨叶与十大功劳叶的鉴别[J].首都医药,2011,18(11):48.
[5] 国家药典委员会.中国药典2010年版:一部[S].北京:中国医药科技出版社,2010:233,附录20,附录18.
[6] 田进国,任健,娄红祥,等.枸骨叶与十大功劳叶红外光谱的研究[J].北京中医药大学学报,1998,21(5):36-38.
[7] 纪秀红,阎玉凝,李家实.枸骨叶与十大功劳叶紫外光谱鉴别[J].中药材,2000,23(3):137-139.
[8] 陈永林,霍德兰,赵华英,等.15种易混淆叶类药材的荧光鉴别研究[J].山东医药工业,1994,13(4):42-43.
[9] 中华人民共和国卫生部药政管理局.全国中药炮制规范:1988年版[M].北京:人民卫生出版社,1988:248.
[10] 何报作,韦,曾静,等.中药叶脉图谱鉴别法[M].黄璐琦,胡之壁.中药鉴定新技术新方法及其应用.北京:人民卫生出版社,2010:32-33.
[11] 何报作,韦,梁慧,等.木亡果叶同伪品扁桃叶的形态及脉序图谱的鉴别特征[J].广西中医学院学报,2005,8(3):90-94.
[12] 何报作,韦,曾静.中药品种真伪鉴别方法可靠性的定量评价初探[J].中成药,2007,29(9):1353-1355.
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