广西师范大学学报（自然科学版） ›› 2012, Vol. 30 ›› Issue (3): 288-294.

• • 上一篇    

4种植物精油的抑菌活性及应用

邓业成, 宁蕾   

  1. 广西师范大学生命科学学院,广西桂林541004
  • 收稿日期:2012-05-27 出版日期:2012-09-20 发布日期:2018-12-04
  • 通讯作者: 邓业成(1965—),男,广西全州人,广西师范大学教授,博士。E-mail:dyecheng@163.com
  • 基金资助:
    广西科学研究与技术开发计划基金资助项目(10169-08);广西自然科学基金资助项目(桂科自0991097)

Inhibitory Activities and Application of Four Plant Essential Oils

DENG Ye-cheng, NING Lei   

  1. College of Life Science,Guangxi Normal University,Guilin Guangxi 541004,China
  • Received:2012-05-27 Online:2012-09-20 Published:2018-12-04

摘要： 采用液体培养基稀释法测定4种植物精油对11种动物病原细菌的最低抑制浓度(MIC),山苍子精油、南丰蜜橘精油、飞蓬精油和胜红蓟精油的MIC分别为0.062 5～0.5、0.125～1、2～4和0.062 5～0.125 mL/L;山苍子精油、南丰蜜橘精油和胜红蓟精油对供试病原细菌的抑菌活性好,飞蓬精油的抑菌活性相对较差。采用气态熏蒸法测定4种植物精油对环境常见霉菌的抑菌效果,当精油剂量为16 μL/皿时,4种植物精油对环境霉菌有抑制作用,山苍子精油和南丰蜜橘精油的抑制效果较好(此2种精油在27 °C下对面包霉变有较好的抑制效果)。通过对植物精油漱口水的配方研究,确定了配方中各成分的最佳配比,并制备出山苍子精油漱口水。用山苍子精油漱口水漱口,对口腔细菌的除菌率为39.96%,与市售漱口水的除菌率相似,在除菌方面达到了工业产品的要求。采用GC/MS技术鉴定了4种植物精油的主要化学成分。

关键词: 植物精油, 动物病原细菌, 抑菌活性, 防腐, 漱口水

Abstract: Inhibitory activities of four plant essential oils against eleven animal pathogenic bacteria and environmental mould were determined using broth dilution method and gaseous fumigation.The results showed that The MICs of oils fromLitsea mollis,Citrus reticulata,Erigeron acer and Ageratum conyzoides to thetested animal pathogenic bacteria were 0.062 5～0.5,0.125～1,2～4 and 0.062 5～0.125 mL/L,respectively.The inhibitory activities ofoils from L.mollis,C.reticulata and E.acer were high,and that of A.conyzoides oil was relatively low.At a dose of 16 μL per utensil,the four plant essential oils had inhibition against environmental mould,and the inhibitory activities of oils from L.mollis and C.reticulata were higher than that of anothertwo oils.The oils of L.mollis and C.reticulata also had inhibitory effect onmould growth of bread at 27 °C and RH 80%.By studying the formulation of mouthwash of plant essential oil,the optimum proportion of all components in the mouthwash was confirmed,and the mouthwash of L.mollis essential oil prepared.Theremoving rate of mouth bacteria by the new mouthwash reached 39.96%,which was similar to the removing rate by commercial mouthwash and met the demand of industrial product.The principal compounds of four plant essential oils were also identified using GC/MS technique.

Key words: plant essential oils, animal pathogenic bacteria, inhibitory activity, antisepsis, mouthwash

中图分类号: 

  • Q946.8
[1] 刘洪波,史冬辉,张瑜.植物精油抗菌活性研究进展[J].畜牧与饲料科学,2009,30(1):12-14.
[2] FAZELI M R,GHOLAMREZA A,AHMADDIAN A M M.Antimicrobial activities of Iranian sumac and avishane-shirazi (Zataria multiflora) against some food-borne bacteria[J].Food Control,2007,8:646-649.
[3] 侯华民,冯俊涛,陈安良,等.植物精油对几种害虫的毒杀活性[J].天然产物研究与开发,2002,14(6):27-30.
[4] 顾仁勇,李俊稷,傅伟昌.连翘精油抑菌及抗氧化作用研究[J].现代食品科技,2008,24(2):120-122.
[5] PITAROKILI D,TZAKOU O,LOUKIS A,et al.Volatile metabolites from Salviafruticosa as antifungal agents in soilborne pathogens[J].Journal of Agricultural and Food Chemistry,2003,51(11):3294-3301.
[6] MUELLER R F,BERGER B,YEGEN O.Chemical composition and fungitoxicproperties to phytopathogenic fungi of essential oils of selected aromatic plants growingwild in Turkey[J].Journal of Agricultural and Food Chemistry,1995,43(8):2262-2266.
[7] 袁敏之.几种植物精油的提取及其在化妆品中的应用[J].日用化学品科学,1996,90(5):39-41.
[8] 吴佩文.植物精油类农药[J].世界农药,1999,21(6):23-27.
[9] 杜芸.天然食品防腐剂的研究进展[J].食品研究与开发,2006,27(3):162-165.
[10] 肖素荣,李京东.天然食品防腐剂及其发展前景[J].中国食物与营养,2007(6):30-33.
[11] 郭双华,陶加乾,陈龙,等.新型漱口水的制备[J].江苏化工,2008,36(3):26-27.
[12] 杨政,梁佩兰.天然功效成分的提取及其在漱口水中的运用[J].香料香精化妆品,2000(1):14-17.
[13] 宁蕾.5种植物精油的生物活性及其应用研究[D].桂林:广西师范大学,2011.
[14] 张均田.现代药理实验方法[M].北京:北京医科大学、中国协和医科大学联合出版社,1998:1409-1415.
[15] 陈冬梅,唐春红.国外天然食品防腐剂的研究进展[J].中国食品添加剂,2008(2):73-77.
[16] 谭龙飞,尤蓉,钟彩莲,等.咖喱粉两种提取物的抑菌作用研究[J].食品科技,2003(12):55-57.
[17] 余伯良.山苍子油对霉菌抗菌性及其与黄曲霉产毒关系的研究[J].微生物学通报,1998,25(3):144-147.
[18] 余伯良,罗惠波,周健,等.山苍子油抗霉菌及抑制黄曲霉产毒的有效成分研究[J].四川轻化工学院学报,2002,15(1):32-36.
[19] 沈妍,傅瑜,陈健初,等.柑橘精油在食品中的抗菌应用[J].食品工业科技,2009,30(6):315-318.
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