2025年04月05日 星期六

广西师范大学学报（自然科学版） ›› 2025, Vol. 43 ›› Issue (2): 238-246.doi: 10.16088/j.issn.1001-6600.2024032606

• 药用资源研究 • 上一篇    下一篇

银杏叶提取物及注射液中黄酮类成分分析与含量测定

胡庆丰1, 冉庆念1, 刘婕1, 王敬赫1, 惠爱玲1,2*, 张文成1,2   

  1. 1.合肥工业大学 食品与生物工程学院, 安徽 合肥 230601;
    2.农产品生物化工教育部工程研究中心(合肥工业大学), 安徽 合肥 230601
  • 收稿日期:2024-03-26 修回日期:2024-04-30 出版日期:2025-03-05 发布日期:2025-04-02
  • 通讯作者: 惠爱玲(1980—), 女, 河南南阳人, 合肥工业大学副教授, 博士。E-mail: haling@hfut.edu.cn
  • 基金资助:
    江苏省苏北科技专项(XZ-SZ202030)

Component Analysis and Content Determination of Characteristic Flavonoids in Ginkgo biloba Extract and Its Injections

HU Qingfeng1, RAN Qingnian1, LIU Jie1, WANG Jinghe1, HUI Ailing1,2*, ZHANG Wencheng1,2   

  1. 1. School of Food and Biological Engineering, Hefei University of Technology, Hefei Anhui 230601, China;
    2. Engineering Research Center of Bio-Process of Ministry of Education (Hefei University of Technology), Hefei Anhui 230601, China
  • Received:2024-03-26 Revised:2024-04-30 Online:2025-03-05 Published:2025-04-02

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摘要： 为研究银杏叶提取物(GBE)及其注射液中特征性黄酮类成分,本文采用HPLC指纹图谱、液质联用等方法对其中共有且主要的黄酮类成分进行分析,并建立HPLC定量检测方法对黄酮含量进行测定。结果表明:GBE及其注射液中芦丁、异槲皮苷、山柰酚-3-O-芸香糖苷、水仙苷、紫云英苷、槲皮素、山柰酚、异鼠李素等8种成分为GBE含量较高且具特征性的黄酮成分;采用C18色谱柱,乙腈-0.2%磷酸梯度洗脱,流速1.0 mL/min,检测波长360 nm,柱温30 ℃进行HPLC定量检测,8种黄酮成分分别在6.25～75.00、1.00～12.00、1.75～21.00、1.75～21.00、1.25～15.00、1.25～15.00、0.50～6.00和0.50～6.00 mg/L范围内线性关系良好(r>0.999),其精密度、重复性、稳定性及加样回收率RSD均小于2%;3种GBE粉末中8种黄酮总含量分别为61.37、76.23、140.55 mg/g,4种GBE注射液的黄酮总含量分别为246.0、305.2、284.5和250.8 mg/L。该研究为高品质GBE原料药及其注射液制品的质量控制提供科学依据。

关键词: 银杏叶提取物, 注射液, HPLC指纹图谱, 液质联用, 黄酮

Abstract: To analyze the characteristic flavonoids in Ginkgo biloba extract (GBE) and its injections, the HPLC fingerprint and liquid chromatography-mass spectrometry (LC-MS) were used to determine the flavonoid components. Additionally, a HPLC quantitative detection method for determining the total flavonoids content was established. Eight flavonoids including rutin, isoquercetin, kaempferol-3-O-rutinoside, narcissus, astragalin, quercetin, kaempferol and isorhamnetin occupied the high content in GBE and injections, which served as the characteristic flavonoid components for further research. The HPLC quantitative detection was performed using a C18 column, acetonitrile-0.2% phosphoric acid as gradient elution, flow rate of 1.0 mL/min, detection wavelength of 360 nm, and column temperature of 30 ℃. Eight flavonoids demonstrated good linear relationship (r > 0.999) when their concentration ranged from 6.25 to 75.00, 1.00 to 12.00, 1.75 to 21.00, 1.75 to 21.00, 1.25 to 15.00, 1.25 to 15.00, 0.50 to 6.00 and 0.50 to 6.00 mg/L, respectively. The precision, repeatability, stability, and recovery rate RSD were less than 2%. The total flavonoid content in three GBEs were 61.37,76.23,140.55 mg/g, respectively. Also, four injections gave the results of 246.0, 305.2, 284.5, 250.8 mg/L. This study identifies eight characteristic flavonoids in GBE and its injections and establishes a quantitative detection method, which will provide an important reference for the quality monitoring of GBE preparations.

Key words: Ginkgo biloba extract, injection, HPLC fingerprint, liquid chromatography-mass spectrometry, flavonoids

中图分类号:  R284.1

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