Journal of Guangxi Normal University(Natural Science Edition) ›› 2011, Vol. 29 ›› Issue (2): 99-103.

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cDNA Cloning and Sequence Analysis of the Myosin Light Chain 1 Gene in Siniperca scherzeri

ZHAO Fa-lan, BING Shi-yu, CHEN Dun-xue, NONG Xiao-xian, LIU Xi-liang   

  1. College of Life Science,Guangxi Normal University,Guilin Guangxi 541004,China
  • Received:2010-12-19 Published:2018-11-19

Abstract: The total RNA was isolated from the skeletal white muscle of Siniperca scherzeri.The myosin light chain 1(MCL1) was amplified using PCR technology,and then was cloned.The length of the myosin light chain 1(MCL1) cDNA is 579 bp,and encodes 192 amino acids,which includes two potential EF-hand structures.The putative protein of MLC1 shows predicted molecular weights of20 778.57 with a theoretical pI of 4.51.Compared with the MLC1 of others reported,the homology of the second potential EF-hand structure of the potential Ca2+ binding domain was well conserved among the MLC1s of all species.

Key words: Siniperca scherzeri, myosin light chain 1, cDNA clone

CLC Number: 

  • Q781
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